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Comparative structural analysis of fruit and stem bromelain from Ananas comosus.
Food Chem 2018; 266:183-191FC

Abstract

Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. Bromelain has been identified as an active component and as a major protease of A. comosus. Bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain. The amino acid sequence and domain analysis of the fruit and stem bromelains demonstrated several differences and similarities of these cysteine protease family members. In addition, analysis of the modelled fruit (BAA21848) and stem (CAA08861) bromelains revealed the presence of unique properties of the predicted structures. Sequence analysis and structural prediction of stem and fruit bromelains of A. comosus along with the comparison of both structures provides a new insight on their distinct properties for industrial application.

Authors+Show Affiliations

Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia. Electronic address: aizinor@ump.edu.my.Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia.Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia.Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30381175

Citation

Ramli, Aizi Nor Mazila, et al. "Comparative Structural Analysis of Fruit and Stem Bromelain From Ananas Comosus." Food Chemistry, vol. 266, 2018, pp. 183-191.
Ramli ANM, Manas NHA, Hamid AAA, et al. Comparative structural analysis of fruit and stem bromelain from Ananas comosus. Food Chem. 2018;266:183-191.
Ramli, A. N. M., Manas, N. H. A., Hamid, A. A. A., Hamid, H. A., & Illias, R. M. (2018). Comparative structural analysis of fruit and stem bromelain from Ananas comosus. Food Chemistry, 266, pp. 183-191. doi:10.1016/j.foodchem.2018.05.125.
Ramli ANM, et al. Comparative Structural Analysis of Fruit and Stem Bromelain From Ananas Comosus. Food Chem. 2018 Nov 15;266:183-191. PubMed PMID: 30381175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative structural analysis of fruit and stem bromelain from Ananas comosus. AU - Ramli,Aizi Nor Mazila, AU - Manas,Nor Hasmaliana Abdul, AU - Hamid,Azzmer Azzar Abdul, AU - Hamid,Hazrulrizawati Abd, AU - Illias,Rosli Md, Y1 - 2018/05/30/ PY - 2017/10/07/received PY - 2018/01/29/revised PY - 2018/05/29/accepted PY - 2018/11/2/entrez PY - 2018/11/2/pubmed PY - 2019/1/30/medline KW - 3D structure prediction KW - Amino acid analysis KW - Fruit bromelain KW - Stem bromelain SP - 183 EP - 191 JF - Food chemistry JO - Food Chem VL - 266 N2 - Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. Bromelain has been identified as an active component and as a major protease of A. comosus. Bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain. The amino acid sequence and domain analysis of the fruit and stem bromelains demonstrated several differences and similarities of these cysteine protease family members. In addition, analysis of the modelled fruit (BAA21848) and stem (CAA08861) bromelains revealed the presence of unique properties of the predicted structures. Sequence analysis and structural prediction of stem and fruit bromelains of A. comosus along with the comparison of both structures provides a new insight on their distinct properties for industrial application. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/30381175/Comparative_structural_analysis_of_fruit_and_stem_bromelain_from_Ananas_comosus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(18)30949-X DB - PRIME DP - Unbound Medicine ER -