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Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice.
BMC Plant Biol. 2018 Nov 01; 18(1):264.BP

Abstract

BACKGROUND

Spotted-leaf mutants are important to reveal programmed cell death and defense-related pathways in rice. We previously characterized the phenotype performance of a rice spotted-leaf mutant spl21 and narrowed down the causal gene locus spl21(t) to an 87-kb region in chromosome 12 by map-based cloning.

RESULT

We showed that a single base substitution from A to G at position 836 in the coding sequence of Oryza sativa beta-1,6-N-acetylglucosaminyl transferase (OsGCNT), effectively mutating Tyr to Cys at position 279 in the translated protein sequence, was responsible for the spotted-leaf phenotype as it could be rescued by functional complementation. Compared to the wild type IR64, the spotted-leaf mutant spl21 exhibited loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence associated genes, which indicated that OsGCNT regulates premature leaf senescence. Moreover, the enhanced resistance to the bacterial leaf blight pathogen Xanthomonas oryzae pv. oryzae, up-regulation of pathogenesis-related genes and increased level of jasmonate which suggested that OsGCNT is a negative regulator of defense response in rice. OsGCNT was expressed constitutively in the leaves, sheaths, stems, roots, and panicles, and OsGCNT-GFP was localized to the Golgi apparatus. High throughput RNA sequencing analysis provided further evidence for the biological effects of loss of OsGCNT function on cell death, premature leaf senescence and enhanced disease resistance in rice. Thus, we demonstrated that the novel OsGCNT regulated rice innate immunity and immunity-associated leaf senescence probably by changing the jasmonate metabolic pathway.

CONCLUSIONS

These results reveal that a novel gene Oryza sativa beta-1,6-N-acetylglucosaminyl transferase (OsGCNT) is responsible for the spotted-leaf mutant spl21, and OsGCNT acts as a negative-regulator mediating defense response and immunity-associated premature leaf senescence probably by activating jasmonate signaling pathway.

Authors+Show Affiliations

State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China.State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China.State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China.State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China.State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China.State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China.State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China.State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China. beishangd@163.com.State Key Laboratory of Rice Biology, Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China. 13989818328@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30382816

Citation

Xu, Xia, et al. "Functional Inactivation of OsGCNT Induces Enhanced Disease Resistance to Xanthomonas Oryzae Pv. Oryzae in Rice." BMC Plant Biology, vol. 18, no. 1, 2018, p. 264.
Xu X, Chen Z, Shi YF, et al. Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice. BMC Plant Biol. 2018;18(1):264.
Xu, X., Chen, Z., Shi, Y. F., Wang, H. M., He, Y., Shi, L., Chen, T., Wu, J. L., & Zhang, X. B. (2018). Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice. BMC Plant Biology, 18(1), 264. https://doi.org/10.1186/s12870-018-1489-9
Xu X, et al. Functional Inactivation of OsGCNT Induces Enhanced Disease Resistance to Xanthomonas Oryzae Pv. Oryzae in Rice. BMC Plant Biol. 2018 Nov 1;18(1):264. PubMed PMID: 30382816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice. AU - Xu,Xia, AU - Chen,Zheng, AU - Shi,Yong-Feng, AU - Wang,Hui-Mei, AU - He,Yan, AU - Shi,Lei, AU - Chen,Ting, AU - Wu,Jian-Li, AU - Zhang,Xiao-Bo, Y1 - 2018/11/01/ PY - 2018/05/24/received PY - 2018/10/17/accepted PY - 2018/11/2/entrez PY - 2018/11/2/pubmed PY - 2018/11/14/medline KW - Defense response KW - Oryza sativa L. KW - OsGCNT KW - Premature leaf senescence KW - Spotted-leaf SP - 264 EP - 264 JF - BMC plant biology JO - BMC Plant Biol. VL - 18 IS - 1 N2 - BACKGROUND: Spotted-leaf mutants are important to reveal programmed cell death and defense-related pathways in rice. We previously characterized the phenotype performance of a rice spotted-leaf mutant spl21 and narrowed down the causal gene locus spl21(t) to an 87-kb region in chromosome 12 by map-based cloning. RESULT: We showed that a single base substitution from A to G at position 836 in the coding sequence of Oryza sativa beta-1,6-N-acetylglucosaminyl transferase (OsGCNT), effectively mutating Tyr to Cys at position 279 in the translated protein sequence, was responsible for the spotted-leaf phenotype as it could be rescued by functional complementation. Compared to the wild type IR64, the spotted-leaf mutant spl21 exhibited loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence associated genes, which indicated that OsGCNT regulates premature leaf senescence. Moreover, the enhanced resistance to the bacterial leaf blight pathogen Xanthomonas oryzae pv. oryzae, up-regulation of pathogenesis-related genes and increased level of jasmonate which suggested that OsGCNT is a negative regulator of defense response in rice. OsGCNT was expressed constitutively in the leaves, sheaths, stems, roots, and panicles, and OsGCNT-GFP was localized to the Golgi apparatus. High throughput RNA sequencing analysis provided further evidence for the biological effects of loss of OsGCNT function on cell death, premature leaf senescence and enhanced disease resistance in rice. Thus, we demonstrated that the novel OsGCNT regulated rice innate immunity and immunity-associated leaf senescence probably by changing the jasmonate metabolic pathway. CONCLUSIONS: These results reveal that a novel gene Oryza sativa beta-1,6-N-acetylglucosaminyl transferase (OsGCNT) is responsible for the spotted-leaf mutant spl21, and OsGCNT acts as a negative-regulator mediating defense response and immunity-associated premature leaf senescence probably by activating jasmonate signaling pathway. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/30382816/Functional_inactivation_of_OsGCNT_induces_enhanced_disease_resistance_to_Xanthomonas_oryzae_pv__oryzae_in_rice_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1489-9 DB - PRIME DP - Unbound Medicine ER -