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Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children.
Front Microbiol 2018; 9:2494FM

Abstract

Mexico is experiencing an epidemiological and nutritional transition period, and Mexican children are often affected by the double burden of malnutrition, which includes undernutrition (15.3% of children) and obesity (13.6%). The gut microbiome is a complex and metabolically active community of organisms that influences the host phenotype. Although previous studies have shown alterations in the gut microbiota in undernourished children, the affected bacterial communities remain unknown. The present study investigated and compared the bacterial richness and diversity of the fecal microbiota in groups of undernourished (n = 12), obese (n = 12), and normal-weight (control) (n = 12) Mexican school-age children. We used next-generation sequencing to analyze the V3-V4 region of the bacterial 16S rRNA gene, and we also investigated whether there were correlations between diet and relevant bacteria. The undernourished and obese groups showed lower bacterial richness and diversity than the normal-weight group. Enterotype 1 correlated positively with dietary fat intake in the obese group and with carbohydrate intake in the undernourished group. The results showed that undernourished children had significantly higher levels of bacteria in the Firmicutes phylum and in the Lachnospiraceae family than obese children, while the Proteobacteria phylum was overrepresented in the obese group. The level of Lachnospiraceae correlated negatively with energy consumption and positively with leptin level. This is the first study to examine the gut microbial community structure in undernourished and obese Mexican children living in low-income neighborhoods. Our analysis revealed distinct taxonomic profiles for undernourished and obese children.

Authors+Show Affiliations

Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, Universidad Nacional Autónoma de México - Instituto Nacional de Medicina Genómica, Mexico City, Mexico.Laboratorio de Endocrinología Molecular, Hospital Juárez de México, Mexico City, Mexico.Facultad de Química, Universidad Nacional Autónoma de México, Mexico City, Mexico.Unidad de Vinculación Científica de la Facultad de Medicina UNAM-INMEGEN, Instituto Nacional de Medicina Genómica, Mexico City, Mexico.Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, Universidad Nacional Autónoma de México - Instituto Nacional de Medicina Genómica, Mexico City, Mexico. Facultad de Química, Universidad Nacional Autónoma de México, Mexico City, Mexico.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30386323

Citation

Méndez-Salazar, Eder Orlando, et al. "Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children." Frontiers in Microbiology, vol. 9, 2018, p. 2494.
Méndez-Salazar EO, Ortiz-López MG, Granados-Silvestre MLÁ, et al. Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children. Front Microbiol. 2018;9:2494.
Méndez-Salazar, E. O., Ortiz-López, M. G., Granados-Silvestre, M. L. Á., Palacios-González, B., & Menjivar, M. (2018). Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children. Frontiers in Microbiology, 9, p. 2494. doi:10.3389/fmicb.2018.02494.
Méndez-Salazar EO, et al. Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children. Front Microbiol. 2018;9:2494. PubMed PMID: 30386323.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered Gut Microbiota and Compositional Changes in Firmicutes and Proteobacteria in Mexican Undernourished and Obese Children. AU - Méndez-Salazar,Eder Orlando, AU - Ortiz-López,María Guadalupe, AU - Granados-Silvestre,María de Los Ángeles, AU - Palacios-González,Berenice, AU - Menjivar,Marta, Y1 - 2018/10/16/ PY - 2017/10/26/received PY - 2018/09/28/accepted PY - 2018/11/3/entrez PY - 2018/11/6/pubmed PY - 2018/11/6/medline KW - Firmicutes KW - Mexican children KW - microbiota KW - obesity KW - undernourished SP - 2494 EP - 2494 JF - Frontiers in microbiology JO - Front Microbiol VL - 9 N2 - Mexico is experiencing an epidemiological and nutritional transition period, and Mexican children are often affected by the double burden of malnutrition, which includes undernutrition (15.3% of children) and obesity (13.6%). The gut microbiome is a complex and metabolically active community of organisms that influences the host phenotype. Although previous studies have shown alterations in the gut microbiota in undernourished children, the affected bacterial communities remain unknown. The present study investigated and compared the bacterial richness and diversity of the fecal microbiota in groups of undernourished (n = 12), obese (n = 12), and normal-weight (control) (n = 12) Mexican school-age children. We used next-generation sequencing to analyze the V3-V4 region of the bacterial 16S rRNA gene, and we also investigated whether there were correlations between diet and relevant bacteria. The undernourished and obese groups showed lower bacterial richness and diversity than the normal-weight group. Enterotype 1 correlated positively with dietary fat intake in the obese group and with carbohydrate intake in the undernourished group. The results showed that undernourished children had significantly higher levels of bacteria in the Firmicutes phylum and in the Lachnospiraceae family than obese children, while the Proteobacteria phylum was overrepresented in the obese group. The level of Lachnospiraceae correlated negatively with energy consumption and positively with leptin level. This is the first study to examine the gut microbial community structure in undernourished and obese Mexican children living in low-income neighborhoods. Our analysis revealed distinct taxonomic profiles for undernourished and obese children. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/30386323/Altered_Gut_Microbiota_and_Compositional_Changes_in_Firmicutes_and_Proteobacteria_in_Mexican_Undernourished_and_Obese_Children_ L2 - https://dx.doi.org/10.3389/fmicb.2018.02494 DB - PRIME DP - Unbound Medicine ER -