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Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia coli.
Front Chem. 2018; 6:486.FC

Abstract

Aqueous two-phase systems (ATPSs) have been widely utilized for liquid-liquid extraction and purification of biomolecules, with some studies also demonstrating their capacity as a biomarker concentration technique for use in diagnostic settings. As the limited polarity range of conventional polymer-based ATPSs can restrict their use, ionic liquid (IL)-based ATPSs have been recently proposed as a promising alternative to polymer-based ATPSs, since ILs are regarded as tunable solvents with excellent solvation capabilities for a variety of natural compounds and proteins. This study demonstrates the first application of IL ATPSs to point-of-care diagnostics. ATPSs consisting of 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4]) and sodium phosphate salt were utilized to quickly concentrate biomarkers prior to detection using the lateral-flow immunoassay (LFA). We found the phase separation speed of the IL ATPS to be very rapid and a significant improvement upon the separation speed of both polymer-salt and micellar ATPSs. This system was successfully applied to both sandwich and competitive LFA formats and enhanced the detection of both Escherichia coli bacteria and the transferrin protein up to 8- and 20-fold, respectively. This system's compatibility with a broad range of biomolecules, rapid phase separation speed, and tunability suggest wide applicability for a large range of different antigens and biomarkers.

Authors+Show Affiliations

Kamei Laboratory, UCLA, Department of Bioengineering, Los Angeles, CA, United States.Kamei Laboratory, UCLA, Department of Bioengineering, Los Angeles, CA, United States.Kamei Laboratory, UCLA, Department of Bioengineering, Los Angeles, CA, United States.Kamei Laboratory, UCLA, Department of Bioengineering, Los Angeles, CA, United States.Kamei Laboratory, UCLA, Department of Bioengineering, Los Angeles, CA, United States.Wu Laboratory, UCLA, Department of Bioengineering, Los Angeles, CA, United States.Kamei Laboratory, UCLA, Department of Bioengineering, Los Angeles, CA, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30386770

Citation

Yee, Matthew F., et al. "Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia Coli." Frontiers in Chemistry, vol. 6, 2018, p. 486.
Yee MF, Emmel GN, Yang EJ, et al. Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia coli. Front Chem. 2018;6:486.
Yee, M. F., Emmel, G. N., Yang, E. J., Lee, E., Paek, J. H., Wu, B. M., & Kamei, D. T. (2018). Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia coli. Frontiers in Chemistry, 6, 486. https://doi.org/10.3389/fchem.2018.00486
Yee MF, et al. Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia Coli. Front Chem. 2018;6:486. PubMed PMID: 30386770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia coli. AU - Yee,Matthew F, AU - Emmel,Grace N, AU - Yang,Eric J, AU - Lee,Eumene, AU - Paek,Justin H, AU - Wu,Benjamin M, AU - Kamei,Daniel T, Y1 - 2018/10/16/ PY - 2018/05/30/received PY - 2018/09/24/accepted PY - 2018/11/3/entrez PY - 2018/11/6/pubmed PY - 2018/11/6/medline KW - Escherichia coli KW - aqueous two-phase systems KW - ionic liquid KW - lateral-flow immunoassay KW - transferrin SP - 486 EP - 486 JF - Frontiers in chemistry JO - Front Chem VL - 6 N2 - Aqueous two-phase systems (ATPSs) have been widely utilized for liquid-liquid extraction and purification of biomolecules, with some studies also demonstrating their capacity as a biomarker concentration technique for use in diagnostic settings. As the limited polarity range of conventional polymer-based ATPSs can restrict their use, ionic liquid (IL)-based ATPSs have been recently proposed as a promising alternative to polymer-based ATPSs, since ILs are regarded as tunable solvents with excellent solvation capabilities for a variety of natural compounds and proteins. This study demonstrates the first application of IL ATPSs to point-of-care diagnostics. ATPSs consisting of 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4]) and sodium phosphate salt were utilized to quickly concentrate biomarkers prior to detection using the lateral-flow immunoassay (LFA). We found the phase separation speed of the IL ATPS to be very rapid and a significant improvement upon the separation speed of both polymer-salt and micellar ATPSs. This system was successfully applied to both sandwich and competitive LFA formats and enhanced the detection of both Escherichia coli bacteria and the transferrin protein up to 8- and 20-fold, respectively. This system's compatibility with a broad range of biomolecules, rapid phase separation speed, and tunability suggest wide applicability for a large range of different antigens and biomarkers. SN - 2296-2646 UR - https://www.unboundmedicine.com/medline/citation/30386770/Ionic_Liquid_Aqueous_Two_Phase_Systems_for_the_Enhanced_Paper_Based_Detection_of_Transferrin_and_Escherichia_coli_ L2 - https://doi.org/10.3389/fchem.2018.00486 DB - PRIME DP - Unbound Medicine ER -
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