Tags

Type your tag names separated by a space and hit enter

Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling.
Int J Mol Med. 2019 Jan; 43(1):185-198.IJ

Abstract

Red Liriope platyphylla extract (EtRLP) has been used as an oriental medicine for treatment of several chronic conditions, such as neurodegenerative disorders, diabetes, and obesity. To investigate the laxative activity of EtRLP, the levels of key constipation markers and their molecular regulators were examined following administration of EtRLP in constipation Sprague Dawley (SD) rats treated with loperamide (Lop). Compared with the Lop+Vehicle‑treated group, the excretion levels of urine and stool were significantly enhanced in the Lop+EtRLP‑treated group, even though feeding levels were kept constant. There was a significant improvement in histological structure, cytological ultrastructure and mucin secretion in transverse colon sections from the Lop+EtRLP‑treated group, compared with the Lop+Vehicle‑treated group. The Lop+EtRLP‑treated group exhibited a rapid recovery of the muscarinic acetylcholine receptor (mAChR) signaling pathway and of the endoplasmic reticulum (ER) stress response, compared with Lop+Vehicle‑treated group. Spicatoside A, one of the key components detected in EtRLP, recovered the levels of inositol triphosphate (IP3) and Gα in primary rat intestinal smooth muscle cells (pRISMCs). Taken together, the present results indicated that spicatoside A‑containing EtRLP had therapeutic effects against Lop‑induced constipation in SD rats via improvement of the mAChR downstream signaling pathway and the ER stress response.

Links

PMC Free PDF

Authors+Show Affiliations

Kim JE
Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627‑706, Republic of Korea.
Go J
Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627‑706, Republic of Korea.
Lee HS
Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 609‑735, Republic of Korea.
Hong JT
College of Pharmacy, Chungbok National University, Chungju 361‑763, Republic of Korea.
Hwang DY
Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627‑706, Republic of Korea.

MeSH

AnimalsColonConstipationDefecationDisease Models, AnimalEndoplasmic Reticulum StressFeeding BehaviorFlavonoidsLaxativesLiriope PlantMaleModels, BiologicalPhenolsRats, Sprague-DawleyReceptors, MuscarinicSaponinsSignal Transduction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30387811

Citation

Kim, Ji Eun, et al. "Spicatoside a in Red Liriope Platyphylla Displays a Laxative Effect in a Constipation Rat Model Via Regulating mAChRs and ER Stress Signaling." International Journal of Molecular Medicine, vol. 43, no. 1, 2019, pp. 185-198.
Kim JE, Go J, Lee HS, et al. Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling. Int J Mol Med. 2019;43(1):185-198.
Kim, J. E., Go, J., Lee, H. S., Hong, J. T., & Hwang, D. Y. (2019). Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling. International Journal of Molecular Medicine, 43(1), 185-198. https://doi.org/10.3892/ijmm.2018.3960
Kim JE, et al. Spicatoside a in Red Liriope Platyphylla Displays a Laxative Effect in a Constipation Rat Model Via Regulating mAChRs and ER Stress Signaling. Int J Mol Med. 2019;43(1):185-198. PubMed PMID: 30387811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling. AU - Kim,Ji Eun, AU - Go,Jun, AU - Lee,Hee Seob, AU - Hong,Jin Tae, AU - Hwang,Dae Youn, Y1 - 2018/10/29/ PY - 2018/05/08/received PY - 2018/10/19/accepted PY - 2018/11/6/pubmed PY - 2019/3/1/medline PY - 2018/11/3/entrez SP - 185 EP - 198 JF - International journal of molecular medicine JO - Int J Mol Med VL - 43 IS - 1 N2 - Red Liriope platyphylla extract (EtRLP) has been used as an oriental medicine for treatment of several chronic conditions, such as neurodegenerative disorders, diabetes, and obesity. To investigate the laxative activity of EtRLP, the levels of key constipation markers and their molecular regulators were examined following administration of EtRLP in constipation Sprague Dawley (SD) rats treated with loperamide (Lop). Compared with the Lop+Vehicle‑treated group, the excretion levels of urine and stool were significantly enhanced in the Lop+EtRLP‑treated group, even though feeding levels were kept constant. There was a significant improvement in histological structure, cytological ultrastructure and mucin secretion in transverse colon sections from the Lop+EtRLP‑treated group, compared with the Lop+Vehicle‑treated group. The Lop+EtRLP‑treated group exhibited a rapid recovery of the muscarinic acetylcholine receptor (mAChR) signaling pathway and of the endoplasmic reticulum (ER) stress response, compared with Lop+Vehicle‑treated group. Spicatoside A, one of the key components detected in EtRLP, recovered the levels of inositol triphosphate (IP3) and Gα in primary rat intestinal smooth muscle cells (pRISMCs). Taken together, the present results indicated that spicatoside A‑containing EtRLP had therapeutic effects against Lop‑induced constipation in SD rats via improvement of the mAChR downstream signaling pathway and the ER stress response. SN - 1791-244X UR - https://www.unboundmedicine.com/medline/citation/30387811/Spicatoside_A_in_red_Liriope_platyphylla_displays_a_laxative_effect_in_a_constipation_rat_model_via_regulating_mAChRs_and_ER_stress_signaling_ DB - PRIME DP - Unbound Medicine ER -