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A new chromanone isolated from Portulaca oleracea L. increases glucose uptake by stimulating GLUT4 translocation to the plasma membrane in 3T3-L1 adipocytes.
Int J Biol Macromol. 2019 Feb 15; 123:26-34.IJ

Abstract

Three homoisoflavonoids and one dimethoxychalcone from Portulaca oleracea L. were isolated using bioassay-guided fractionation and HPLC. Among the compounds 1-4, (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone (compound 3) had the most effect on glucose uptake in the adipocytes. We investigated how (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone contributed to increase glucose uptake in 3T3-L1 adipocytes. Levels of the glucose transporters GLUT-4, as well as glucose uptake, and key proteins of the insulin pathway, namely PI3K/AKT and AMPK pathway are analyzed using glucose uptake assay and western blot analysis. Our results show that (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone significantly increased glucose uptake by stimulating translocation of GLUT4 to the plasma membrane in 3T3-L1 adipocytes. High levels of expression of GLUT4 in the plasma membrane resulted from IRS-1 phosphorylation, PI3K activation, Akt phosphorylation and phosphorylation of AMPK, resulting in increased glucose uptake by the cells. The increase in glucose uptake due to (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone was significantly inhibited by the PI3K inhibitor and the AMPK inhibitor in 3T3-L1 adipocytes. These findings suggest that (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone may increase glucose uptake by stimulating GLUT4 translocation to the plasma membrane via activating the PI3K/Akt and AMPK pathways in 3T3-L1 adipocytes.

Authors+Show Affiliations

Department of Food Science and Nutrition, Pusan National University, Busan 46241, Republic of Korea.Department of Food Science and Nutrition, Pusan National University, Busan 46241, Republic of Korea.Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 49112, Republic of Korea. Electronic address: ywseo@hhu.ac.kr.Department of Food Science and Nutrition, Pusan National University, Busan 46241, Republic of Korea. Electronic address: hanjs@pusan.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30389528

Citation

Park, Jae Eun, et al. "A New Chromanone Isolated From Portulaca Oleracea L. Increases Glucose Uptake By Stimulating GLUT4 Translocation to the Plasma Membrane in 3T3-L1 Adipocytes." International Journal of Biological Macromolecules, vol. 123, 2019, pp. 26-34.
Park JE, Park JY, Seo Y, et al. A new chromanone isolated from Portulaca oleracea L. increases glucose uptake by stimulating GLUT4 translocation to the plasma membrane in 3T3-L1 adipocytes. Int J Biol Macromol. 2019;123:26-34.
Park, J. E., Park, J. Y., Seo, Y., & Han, J. S. (2019). A new chromanone isolated from Portulaca oleracea L. increases glucose uptake by stimulating GLUT4 translocation to the plasma membrane in 3T3-L1 adipocytes. International Journal of Biological Macromolecules, 123, 26-34. https://doi.org/10.1016/j.ijbiomac.2018.10.206
Park JE, et al. A New Chromanone Isolated From Portulaca Oleracea L. Increases Glucose Uptake By Stimulating GLUT4 Translocation to the Plasma Membrane in 3T3-L1 Adipocytes. Int J Biol Macromol. 2019 Feb 15;123:26-34. PubMed PMID: 30389528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new chromanone isolated from Portulaca oleracea L. increases glucose uptake by stimulating GLUT4 translocation to the plasma membrane in 3T3-L1 adipocytes. AU - Park,Jae Eun, AU - Park,Ji Yeon, AU - Seo,Youngwan, AU - Han,Ji Sook, Y1 - 2018/10/30/ PY - 2018/07/18/received PY - 2018/10/29/revised PY - 2018/10/29/accepted PY - 2018/11/6/pubmed PY - 2019/4/11/medline PY - 2018/11/4/entrez KW - 3T3-L1 adipocytes KW - AMPK KW - Diabetes KW - Glucose uptake KW - Homoisoflavonoid KW - PI3K/Akt SP - 26 EP - 34 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 123 N2 - Three homoisoflavonoids and one dimethoxychalcone from Portulaca oleracea L. were isolated using bioassay-guided fractionation and HPLC. Among the compounds 1-4, (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone (compound 3) had the most effect on glucose uptake in the adipocytes. We investigated how (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone contributed to increase glucose uptake in 3T3-L1 adipocytes. Levels of the glucose transporters GLUT-4, as well as glucose uptake, and key proteins of the insulin pathway, namely PI3K/AKT and AMPK pathway are analyzed using glucose uptake assay and western blot analysis. Our results show that (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone significantly increased glucose uptake by stimulating translocation of GLUT4 to the plasma membrane in 3T3-L1 adipocytes. High levels of expression of GLUT4 in the plasma membrane resulted from IRS-1 phosphorylation, PI3K activation, Akt phosphorylation and phosphorylation of AMPK, resulting in increased glucose uptake by the cells. The increase in glucose uptake due to (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone was significantly inhibited by the PI3K inhibitor and the AMPK inhibitor in 3T3-L1 adipocytes. These findings suggest that (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone may increase glucose uptake by stimulating GLUT4 translocation to the plasma membrane via activating the PI3K/Akt and AMPK pathways in 3T3-L1 adipocytes. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/30389528/A_new_chromanone_isolated_from_Portulaca_oleracea_L__increases_glucose_uptake_by_stimulating_GLUT4_translocation_to_the_plasma_membrane_in_3T3_L1_adipocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)33687-0 DB - PRIME DP - Unbound Medicine ER -