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Stability of Vegetal Diamine Oxidase in Simulated Intestinal Media: Protective Role of Cholic Acids.
J Agric Food Chem. 2018 Dec 05; 66(48):12657-12665.JA

Abstract

Food biogenic amines, in particular, histamine, are often responsible for various enteric and vascular dysfunctions. Several years ago, the oral administration of copper-containing diamine oxidase (DAO), also called histaminase, able to oxidatively deaminate biogenic amines, had been suggested as a food supplement to control food allergy and enteric dysfunctions. This report is aimed to generate a global image on the behavior of orally administrated DAO dosage forms in the intestinal tract. The catalytic stability of DAO from Lathyrus sativus seedlings in various simulated intestinal media with different pH and containing different association of cholic acids, pancreatic proteases, bicarbonate, lipids, or alcohol was investigated. Cholic acids and lipids protected the enzyme in the simulated intestinal fluids. However, they were not able to protect against the inhibitory effect of 24-36% (v/v) ethanol. These observations may be relevant for oral administration of enzymes as food supplements or therapeutic bioactive agents.

Authors+Show Affiliations

Department of Chemistry, Research Chair on Enteric Dysfunctions "Allerdys" and Centre Pharmaqam , Université du Québec à Montréal , Montréal , Québec H3C 3P8 , Canada.Department of Biochemical Sciences "A. Rossi Fanelli" , Sapienza University of Rome , 00185 Rome , Italy.Department of Chemistry, Research Chair on Enteric Dysfunctions "Allerdys" and Centre Pharmaqam , Université du Québec à Montréal , Montréal , Québec H3C 3P8 , Canada.Department of Chemistry, Research Chair on Enteric Dysfunctions "Allerdys" and Centre Pharmaqam , Université du Québec à Montréal , Montréal , Québec H3C 3P8 , Canada.Department of Biochemical Sciences "A. Rossi Fanelli" , Sapienza University of Rome , 00185 Rome , Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30398054

Citation

Neree, Armelle Tchoumi, et al. "Stability of Vegetal Diamine Oxidase in Simulated Intestinal Media: Protective Role of Cholic Acids." Journal of Agricultural and Food Chemistry, vol. 66, no. 48, 2018, pp. 12657-12665.
Neree AT, Pietrangeli P, Szabo PI, et al. Stability of Vegetal Diamine Oxidase in Simulated Intestinal Media: Protective Role of Cholic Acids. J Agric Food Chem. 2018;66(48):12657-12665.
Neree, A. T., Pietrangeli, P., Szabo, P. I., Mateescu, M. A., & Marcocci, L. (2018). Stability of Vegetal Diamine Oxidase in Simulated Intestinal Media: Protective Role of Cholic Acids. Journal of Agricultural and Food Chemistry, 66(48), 12657-12665. https://doi.org/10.1021/acs.jafc.8b04005
Neree AT, et al. Stability of Vegetal Diamine Oxidase in Simulated Intestinal Media: Protective Role of Cholic Acids. J Agric Food Chem. 2018 Dec 5;66(48):12657-12665. PubMed PMID: 30398054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability of Vegetal Diamine Oxidase in Simulated Intestinal Media: Protective Role of Cholic Acids. AU - Neree,Armelle Tchoumi, AU - Pietrangeli,Paola, AU - Szabo,Pompilia Ispas, AU - Mateescu,Mircea Alexandru, AU - Marcocci,Lucia, Y1 - 2018/11/20/ PY - 2018/11/7/pubmed PY - 2019/4/19/medline PY - 2018/11/7/entrez KW - IBD KW - cholic acids KW - diamine oxidase KW - enteric dysfunctions KW - ethanol KW - food allergy KW - food supplements KW - histaminase KW - histamine KW - oral drug formulation KW - protein stability KW - simulated intestinal fluids KW - sodium bicarbonate KW - vegetal DAO SP - 12657 EP - 12665 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 66 IS - 48 N2 - Food biogenic amines, in particular, histamine, are often responsible for various enteric and vascular dysfunctions. Several years ago, the oral administration of copper-containing diamine oxidase (DAO), also called histaminase, able to oxidatively deaminate biogenic amines, had been suggested as a food supplement to control food allergy and enteric dysfunctions. This report is aimed to generate a global image on the behavior of orally administrated DAO dosage forms in the intestinal tract. The catalytic stability of DAO from Lathyrus sativus seedlings in various simulated intestinal media with different pH and containing different association of cholic acids, pancreatic proteases, bicarbonate, lipids, or alcohol was investigated. Cholic acids and lipids protected the enzyme in the simulated intestinal fluids. However, they were not able to protect against the inhibitory effect of 24-36% (v/v) ethanol. These observations may be relevant for oral administration of enzymes as food supplements or therapeutic bioactive agents. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/30398054/Stability_of_Vegetal_Diamine_Oxidase_in_Simulated_Intestinal_Media:_Protective_Role_of_Cholic_Acids_ L2 - https://dx.doi.org/10.1021/acs.jafc.8b04005 DB - PRIME DP - Unbound Medicine ER -