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Highly expressed miR-182-5p can promote preeclampsia progression by degrading RND3 and inhibiting HTR-8/SVneo cell invasion.
Eur Rev Med Pharmacol Sci 2018; 22(20):6583-6590ER

Abstract

OBJECTIVE

The role of miR-182-5p in preeclampsia was studied, and its mechanism was also explored.

PATIENTS AND METHODS

Fifty patients with preeclampsia were assigned to the study group and 50 normal pregnant women to the control group. The age, weight, blood pressure, urinary protein, and weight of newborns were compared between the two groups. The placental tissues of the above-mentioned subjects were collected, and quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) assay was used to detect the expression of miR-182-5p. MiR-182-5p was overexpressed or knocked down using a cell transfection assay in HTR-8/SVneov cell, which is a kind of human chorionic trophoblast cell. Changes in cell migration and invasiveness before and after transfection were determined by wound healing test and transwell assay, respectively. Western blot was performed to analyze the change of RND3 protein level before and after transfection. The biological prediction of the relationship between miR-182-5p and RND3 was performed and a dual luciferase reporter gene experiment was designed to verify the results. Finally, a rescue experiment was conducted to investigate whether RND3 could affect the role of miRNA-182-5p in the capacity of cell migration and invasion.

RESULTS

Preeclampsia patients had higher systolic blood pressure, diastolic blood pressure, and urinary protein than normal pregnant women, while neonatal weight decreased compared with normal pregnant women. MiRNA-182-5p was highly expressed in placental tissues of patients with preeclampsia. After miRNA-182-5p was overexpressed, the migration and invasion of HTR-8/SVneo cells were significantly attenuated, and the mRNA and protein levels of RND3 were markedly downregulated, and vice versa. The dual luciferase reporting assay confirmed that miRNA-182-5p could bind to 3'UTR of RND3. In addition, the results of rescue experiment showed that overexpressing miRNA-182-5p could markedly inhibit the migration and invasion of HTR-8/SVneo cells; however, when RND3 was simultaneously overexpressed, the inhibitory effect of miRNA-182-5p was partially reversed.

CONCLUSIONS

The highly expressed miRNA-182-5p in patients with preeclampsia promoted the development of preeclampsia, the possible mechanism of which might be that the increased miRNA-182-5p expression could inhibit the migratory and invasive ability of trophoblast cells through targeted degrading RND3 protein.

Authors+Show Affiliations

Department of Obstetrical, The First People's Hospital of Jining, Jining, China. bjzzcb88@163.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30402829

Citation

Fang, Y-N, et al. "Highly Expressed miR-182-5p Can Promote Preeclampsia Progression By Degrading RND3 and Inhibiting HTR-8/SVneo Cell Invasion." European Review for Medical and Pharmacological Sciences, vol. 22, no. 20, 2018, pp. 6583-6590.
Fang YN, Huang ZL, Li H, et al. Highly expressed miR-182-5p can promote preeclampsia progression by degrading RND3 and inhibiting HTR-8/SVneo cell invasion. Eur Rev Med Pharmacol Sci. 2018;22(20):6583-6590.
Fang, Y. N., Huang, Z. L., Li, H., Tan, W. B., Zhang, Q. G., Wang, L., & Wu, J. L. (2018). Highly expressed miR-182-5p can promote preeclampsia progression by degrading RND3 and inhibiting HTR-8/SVneo cell invasion. European Review for Medical and Pharmacological Sciences, 22(20), pp. 6583-6590. doi:10.26355/eurrev_201810_16132.
Fang YN, et al. Highly Expressed miR-182-5p Can Promote Preeclampsia Progression By Degrading RND3 and Inhibiting HTR-8/SVneo Cell Invasion. Eur Rev Med Pharmacol Sci. 2018;22(20):6583-6590. PubMed PMID: 30402829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly expressed miR-182-5p can promote preeclampsia progression by degrading RND3 and inhibiting HTR-8/SVneo cell invasion. AU - Fang,Y-N, AU - Huang,Z-L, AU - Li,H, AU - Tan,W-B, AU - Zhang,Q-G, AU - Wang,L, AU - Wu,J-L, PY - 2018/11/8/entrez PY - 2018/11/8/pubmed PY - 2018/11/8/medline SP - 6583 EP - 6590 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 22 IS - 20 N2 - OBJECTIVE: The role of miR-182-5p in preeclampsia was studied, and its mechanism was also explored. PATIENTS AND METHODS: Fifty patients with preeclampsia were assigned to the study group and 50 normal pregnant women to the control group. The age, weight, blood pressure, urinary protein, and weight of newborns were compared between the two groups. The placental tissues of the above-mentioned subjects were collected, and quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) assay was used to detect the expression of miR-182-5p. MiR-182-5p was overexpressed or knocked down using a cell transfection assay in HTR-8/SVneov cell, which is a kind of human chorionic trophoblast cell. Changes in cell migration and invasiveness before and after transfection were determined by wound healing test and transwell assay, respectively. Western blot was performed to analyze the change of RND3 protein level before and after transfection. The biological prediction of the relationship between miR-182-5p and RND3 was performed and a dual luciferase reporter gene experiment was designed to verify the results. Finally, a rescue experiment was conducted to investigate whether RND3 could affect the role of miRNA-182-5p in the capacity of cell migration and invasion. RESULTS: Preeclampsia patients had higher systolic blood pressure, diastolic blood pressure, and urinary protein than normal pregnant women, while neonatal weight decreased compared with normal pregnant women. MiRNA-182-5p was highly expressed in placental tissues of patients with preeclampsia. After miRNA-182-5p was overexpressed, the migration and invasion of HTR-8/SVneo cells were significantly attenuated, and the mRNA and protein levels of RND3 were markedly downregulated, and vice versa. The dual luciferase reporting assay confirmed that miRNA-182-5p could bind to 3'UTR of RND3. In addition, the results of rescue experiment showed that overexpressing miRNA-182-5p could markedly inhibit the migration and invasion of HTR-8/SVneo cells; however, when RND3 was simultaneously overexpressed, the inhibitory effect of miRNA-182-5p was partially reversed. CONCLUSIONS: The highly expressed miRNA-182-5p in patients with preeclampsia promoted the development of preeclampsia, the possible mechanism of which might be that the increased miRNA-182-5p expression could inhibit the migratory and invasive ability of trophoblast cells through targeted degrading RND3 protein. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/30402829/Highly_expressed_miR_182_5p_can_promote_preeclampsia_progression_by_degrading_RND3_and_inhibiting_HTR_8/SVneo_cell_invasion_ L2 - https://www.europeanreview.org/article/16132 DB - PRIME DP - Unbound Medicine ER -