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Protective Effect of Anthocyanin on Paraquat-Induced Apoptosis and Epithelial-Mesenchymal Transition in Alveolar Type II Cells.
Med Sci Monit. 2018 Nov 07; 24:7980-7987.MS

Abstract

BACKGROUND

Paraquat (PQ) can over-accumulate in alveolar epithelial cells. Anthocyanin (An) can exert anti-oxidative properties. The role of An in PQ-induced toxicity is unclear, so we aimed to explore whether An could inhibit epithelial mesenchymal transition (EMT) induced by PQ in alveolar cells. MATERIAL AND

METHODS

lveolar epithelial cells were treated with PQ and An with concentration gradient for 12, 24, and 48 h. The cell viability, ROS level, and apoptosis rate were determined using the Cell Counting Kit-8 (CCK-8) and flow cytometry, respectively. The lactate dehydrogenase (LDH) leakage, methane dicarboxylic aldehyde (MDA) level, glutathione peroxidase (GPx), and superoxide dismutase (SOD) activities were determined by spectrophotometric method. The mRNA and protein expressions were detected using quantitative real-time PCR (qPCR) and Western blot, respectively.

RESULTS

An reduced the PQ-induced apoptosis in a dose-dependent manner. Moreover, An reduced the ratio of Bax/Bcl-2 to ROS level. We found that An suppressed the activity of LDH and MDA and improved SOD and GPX levels. Additionally, the level of PQ-induced E-cadherin was decreased by An while the expressions of vimentin, α-smooth muscle actin (α-SMA), and collagens type I (col-I) were increased. Furthermore, An inhibited the levels of transforming growth factor β1 (TGF-β1) and activin receptor-like kinase 5 (ALK5) and reduced the phosphorylation of smad2.

CONCLUSIONS

Our study shows newly discovered effects of anthocyanidins on EMT and supports their chemopreventive effects in paraquat-induced apoptosis in alveolar type II cells.

Authors+Show Affiliations

Emergency Department of Traumatology, Shanghai Pudong Hospital, Shanghai, China (mainland).Emergency Department of Traumatology, Shanghai Pudong Hospital, Shanghai, China (mainland).Emergency Department of Traumatology, Shanghai Pudong Hospital, Shanghai, China (mainland).Emergency Department of Traumatology, Shanghai Pudong Hospital, Shanghai, China (mainland).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30403199

Citation

Wang, Zhihua, et al. "Protective Effect of Anthocyanin On Paraquat-Induced Apoptosis and Epithelial-Mesenchymal Transition in Alveolar Type II Cells." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 24, 2018, pp. 7980-7987.
Wang Z, Gu D, Sheng L, et al. Protective Effect of Anthocyanin on Paraquat-Induced Apoptosis and Epithelial-Mesenchymal Transition in Alveolar Type II Cells. Med Sci Monit. 2018;24:7980-7987.
Wang, Z., Gu, D., Sheng, L., & Cai, J. (2018). Protective Effect of Anthocyanin on Paraquat-Induced Apoptosis and Epithelial-Mesenchymal Transition in Alveolar Type II Cells. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 7980-7987. https://doi.org/10.12659/MSM.910730
Wang Z, et al. Protective Effect of Anthocyanin On Paraquat-Induced Apoptosis and Epithelial-Mesenchymal Transition in Alveolar Type II Cells. Med Sci Monit. 2018 Nov 7;24:7980-7987. PubMed PMID: 30403199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective Effect of Anthocyanin on Paraquat-Induced Apoptosis and Epithelial-Mesenchymal Transition in Alveolar Type II Cells. AU - Wang,Zhihua, AU - Gu,Dongming, AU - Sheng,Lezhi, AU - Cai,Jinfang, Y1 - 2018/11/07/ PY - 2018/11/8/entrez PY - 2018/11/8/pubmed PY - 2019/1/8/medline SP - 7980 EP - 7987 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med Sci Monit VL - 24 N2 - BACKGROUND Paraquat (PQ) can over-accumulate in alveolar epithelial cells. Anthocyanin (An) can exert anti-oxidative properties. The role of An in PQ-induced toxicity is unclear, so we aimed to explore whether An could inhibit epithelial mesenchymal transition (EMT) induced by PQ in alveolar cells. MATERIAL AND METHODS lveolar epithelial cells were treated with PQ and An with concentration gradient for 12, 24, and 48 h. The cell viability, ROS level, and apoptosis rate were determined using the Cell Counting Kit-8 (CCK-8) and flow cytometry, respectively. The lactate dehydrogenase (LDH) leakage, methane dicarboxylic aldehyde (MDA) level, glutathione peroxidase (GPx), and superoxide dismutase (SOD) activities were determined by spectrophotometric method. The mRNA and protein expressions were detected using quantitative real-time PCR (qPCR) and Western blot, respectively. RESULTS An reduced the PQ-induced apoptosis in a dose-dependent manner. Moreover, An reduced the ratio of Bax/Bcl-2 to ROS level. We found that An suppressed the activity of LDH and MDA and improved SOD and GPX levels. Additionally, the level of PQ-induced E-cadherin was decreased by An while the expressions of vimentin, α-smooth muscle actin (α-SMA), and collagens type I (col-I) were increased. Furthermore, An inhibited the levels of transforming growth factor β1 (TGF-β1) and activin receptor-like kinase 5 (ALK5) and reduced the phosphorylation of smad2. CONCLUSIONS Our study shows newly discovered effects of anthocyanidins on EMT and supports their chemopreventive effects in paraquat-induced apoptosis in alveolar type II cells. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/30403199/Protective_Effect_of_Anthocyanin_on_Paraquat_Induced_Apoptosis_and_Epithelial_Mesenchymal_Transition_in_Alveolar_Type_II_Cells_ L2 - https://www.medscimonit.com/download/index/idArt/910730 DB - PRIME DP - Unbound Medicine ER -