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Germination and Early Seedling Development in Quercus ilex Recalcitrant and Non-dormant Seeds: Targeted Transcriptional, Hormonal, and Sugar Analysis.
Front Plant Sci. 2018; 9:1508.FP

Abstract

Seed germination and early seedling development have been studied in the recalcitrant species Quercus ilex using targeted transcriptional, hormonal, and sugar analysis. Embryos and seedlings were collected at eight morphologically defined developmental stages, S0-S7. A typical triphasic water uptake curve was observed throughout development, accompanied by a decrease in sucrose and an increase in glucose and fructose. Low levels of abscisic acid (ABA) and high levels of gibberellins (GAs) were observed in mature seeds. Post-germination, indole-3-acetic acid (IAA), increased, whereas GA remained high, a pattern commonly observed during growth and development. The abundance of transcripts from ABA-related genes was positively correlated with the changes in the content of the phytohormone. Transcripts of the drought-related genes Dhn3 and GolS were more abundant at S0, then decreased in parallel with increasing water content. Transcripts for Gapdh, and Nadh6 were abundant at S0, supporting the occurrence of an active metabolism in recalcitrant seeds at the time of shedding. The importance of ROS during germination is manifest in the high transcript levels for Sod and Gst, found in mature seeds. The results presented herein help distinguish recalcitrant (e.g., Q. ilex) seeds from their orthodox counterparts. Our results indicate that recalcitrance is established during seed development but not manifest until germination (S1-S3). Post-germination the patterns are quite similar for both orthodox and recalcitrant seeds.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Agrifood Campus of International Excellence, University of Córdoba, Córdoba, Spain. Departamento de Química Biológica, Dirección de Investigación, Facultad de Ciencias Químicas, Universidad Nacional de Asunción, San Lorenzo, Paraguay. Centro Multidisciplinario de Investigaciones Tecnológicas, Dirección General de Investigación Científica y Tecnológica, Universidad Nacional de Asunción, San Lorenzo, Paraguay.Department of Biochemistry and Molecular Biology, Agrifood Campus of International Excellence, University of Córdoba, Córdoba, Spain. Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Campus de Excelencia Internacional Agroalimentario, Córdoba, Spain.Department of Biochemistry and Molecular Biology, Agrifood Campus of International Excellence, University of Córdoba, Córdoba, Spain.Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Seville, Spain.Plant Physiology Lab, Department of Organisms and Systems Biology, Faculty of Biology, University of Oviedo, Oviedo, Spain.Department of Biochemistry and Molecular Biology, Agrifood Campus of International Excellence, University of Córdoba, Córdoba, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30405659

Citation

Romero-Rodríguez, M Cristina, et al. "Germination and Early Seedling Development in Quercus Ilex Recalcitrant and Non-dormant Seeds: Targeted Transcriptional, Hormonal, and Sugar Analysis." Frontiers in Plant Science, vol. 9, 2018, p. 1508.
Romero-Rodríguez MC, Archidona-Yuste A, Abril N, et al. Germination and Early Seedling Development in Quercus ilex Recalcitrant and Non-dormant Seeds: Targeted Transcriptional, Hormonal, and Sugar Analysis. Front Plant Sci. 2018;9:1508.
Romero-Rodríguez, M. C., Archidona-Yuste, A., Abril, N., Gil-Serrano, A. M., Meijón, M., & Jorrín-Novo, J. V. (2018). Germination and Early Seedling Development in Quercus ilex Recalcitrant and Non-dormant Seeds: Targeted Transcriptional, Hormonal, and Sugar Analysis. Frontiers in Plant Science, 9, 1508. https://doi.org/10.3389/fpls.2018.01508
Romero-Rodríguez MC, et al. Germination and Early Seedling Development in Quercus Ilex Recalcitrant and Non-dormant Seeds: Targeted Transcriptional, Hormonal, and Sugar Analysis. Front Plant Sci. 2018;9:1508. PubMed PMID: 30405659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Germination and Early Seedling Development in Quercus ilex Recalcitrant and Non-dormant Seeds: Targeted Transcriptional, Hormonal, and Sugar Analysis. AU - Romero-Rodríguez,M Cristina, AU - Archidona-Yuste,Antonio, AU - Abril,Nieves, AU - Gil-Serrano,Antonio M, AU - Meijón,Mónica, AU - Jorrín-Novo,Jesús V, Y1 - 2018/10/22/ PY - 2018/06/01/received PY - 2018/09/26/accepted PY - 2018/11/9/entrez PY - 2018/11/9/pubmed PY - 2018/11/9/medline KW - Quercusilex KW - early seedling growth KW - germination KW - phytohormone KW - recalcitrant SP - 1508 EP - 1508 JF - Frontiers in plant science JO - Front Plant Sci VL - 9 N2 - Seed germination and early seedling development have been studied in the recalcitrant species Quercus ilex using targeted transcriptional, hormonal, and sugar analysis. Embryos and seedlings were collected at eight morphologically defined developmental stages, S0-S7. A typical triphasic water uptake curve was observed throughout development, accompanied by a decrease in sucrose and an increase in glucose and fructose. Low levels of abscisic acid (ABA) and high levels of gibberellins (GAs) were observed in mature seeds. Post-germination, indole-3-acetic acid (IAA), increased, whereas GA remained high, a pattern commonly observed during growth and development. The abundance of transcripts from ABA-related genes was positively correlated with the changes in the content of the phytohormone. Transcripts of the drought-related genes Dhn3 and GolS were more abundant at S0, then decreased in parallel with increasing water content. Transcripts for Gapdh, and Nadh6 were abundant at S0, supporting the occurrence of an active metabolism in recalcitrant seeds at the time of shedding. The importance of ROS during germination is manifest in the high transcript levels for Sod and Gst, found in mature seeds. The results presented herein help distinguish recalcitrant (e.g., Q. ilex) seeds from their orthodox counterparts. Our results indicate that recalcitrance is established during seed development but not manifest until germination (S1-S3). Post-germination the patterns are quite similar for both orthodox and recalcitrant seeds. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/30405659/Germination_and_Early_Seedling_Development_in_Quercus_ilex_Recalcitrant_and_Non_dormant_Seeds:_Targeted_Transcriptional_Hormonal_and_Sugar_Analysis_ L2 - https://doi.org/10.3389/fpls.2018.01508 DB - PRIME DP - Unbound Medicine ER -
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