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Expression Profiles of Selenium-Related Genes in Human Chondrocytes Exposed to T-2 Toxin and Deoxynivalenol.
Biol Trace Elem Res. 2019 Aug; 190(2):295-302.BT

Abstract

The combination of excess mycotoxin exposure and selenium deficiency has been widely considered as a cause of Kashin-Beck disease (KBD). The present study aimed to investigate the expression profiles of selenium-related genes in human chondrocytes after exposure to T-2 toxin and deoxynivalenol (DON) and to preliminarily identify the potential biological functions of the identified genes. Gene expression profiling was performed on human chondrocytes treated with 0.01 μg/ml T-2 toxin and 1.0 μg/ml DON by using Affymetrix Human Gene Arrays. The 1660 selenium-related genes were derived from the Comparative Toxicogenomics Database. Gene-term enrichment analysis was conducted by the DAVID gene annotation tool. Our results showed that 69 and 191 selenium-related genes were differentially expressed after T-2 toxin and DON treatment, respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these identified genes were involved in various biological functions, such as the GO terms in response to oxidative stress, cell cycle arrest, and apoptotic process and the KEGG metabolic, FoxO signaling, and p53 signaling pathways. Our results may help explain the mechanisms of interaction between mycotoxins and selenium following human chondrocyte damage and reveal the potential roles of environmental risk factors in cartilage lesions during KBD development.

Authors+Show Affiliations

School of Nursing, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China. School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.Hainan Medical University, Haikou, 571199, Hainan, People's Republic of China.School of Nursing, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.Traditional Chinese Medical Hospital of Linyou County, Baoji, 721500, Shaanxi, People's Republic of China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China. guox@mail.xjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30406490

Citation

Yang, Lei, et al. "Expression Profiles of Selenium-Related Genes in Human Chondrocytes Exposed to T-2 Toxin and Deoxynivalenol." Biological Trace Element Research, vol. 190, no. 2, 2019, pp. 295-302.
Yang L, Zhang J, Li X, et al. Expression Profiles of Selenium-Related Genes in Human Chondrocytes Exposed to T-2 Toxin and Deoxynivalenol. Biol Trace Elem Res. 2019;190(2):295-302.
Yang, L., Zhang, J., Li, X., Xu, C., Wang, X., & Guo, X. (2019). Expression Profiles of Selenium-Related Genes in Human Chondrocytes Exposed to T-2 Toxin and Deoxynivalenol. Biological Trace Element Research, 190(2), 295-302. https://doi.org/10.1007/s12011-018-1560-2
Yang L, et al. Expression Profiles of Selenium-Related Genes in Human Chondrocytes Exposed to T-2 Toxin and Deoxynivalenol. Biol Trace Elem Res. 2019;190(2):295-302. PubMed PMID: 30406490.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression Profiles of Selenium-Related Genes in Human Chondrocytes Exposed to T-2 Toxin and Deoxynivalenol. AU - Yang,Lei, AU - Zhang,Jianping, AU - Li,Xiaomei, AU - Xu,Chao, AU - Wang,Xi, AU - Guo,Xiong, Y1 - 2018/11/07/ PY - 2018/05/27/received PY - 2018/10/25/accepted PY - 2018/11/9/pubmed PY - 2018/11/9/medline PY - 2018/11/9/entrez KW - Chondrocyte KW - Deoxynivalenol KW - Microarray KW - Selenium KW - T-2 toxin SP - 295 EP - 302 JF - Biological trace element research JO - Biol Trace Elem Res VL - 190 IS - 2 N2 - The combination of excess mycotoxin exposure and selenium deficiency has been widely considered as a cause of Kashin-Beck disease (KBD). The present study aimed to investigate the expression profiles of selenium-related genes in human chondrocytes after exposure to T-2 toxin and deoxynivalenol (DON) and to preliminarily identify the potential biological functions of the identified genes. Gene expression profiling was performed on human chondrocytes treated with 0.01 μg/ml T-2 toxin and 1.0 μg/ml DON by using Affymetrix Human Gene Arrays. The 1660 selenium-related genes were derived from the Comparative Toxicogenomics Database. Gene-term enrichment analysis was conducted by the DAVID gene annotation tool. Our results showed that 69 and 191 selenium-related genes were differentially expressed after T-2 toxin and DON treatment, respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these identified genes were involved in various biological functions, such as the GO terms in response to oxidative stress, cell cycle arrest, and apoptotic process and the KEGG metabolic, FoxO signaling, and p53 signaling pathways. Our results may help explain the mechanisms of interaction between mycotoxins and selenium following human chondrocyte damage and reveal the potential roles of environmental risk factors in cartilage lesions during KBD development. SN - 1559-0720 UR - https://www.unboundmedicine.com/medline/citation/30406490/Expression_Profiles_of_Selenium_Related_Genes_in_Human_Chondrocytes_Exposed_to_T_2_Toxin_and_Deoxynivalenol_ L2 - https://dx.doi.org/10.1007/s12011-018-1560-2 DB - PRIME DP - Unbound Medicine ER -