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Positive endocochlear potential: mechanism of production by marginal cells of stria vascularis.
Hear Res. 1987; 29(2-3):117-24.HR

Abstract

The positive endocochlear potential (EP+) and high K+ concentration of the endolymph in the scala media of the mammalian cochlea are unusual. They have long been assumed to be due to a putative K-pump in the luminal membrane of the marginal cells of the stria vascularis, which were believed to have a negative internal potential. We show that the cell potential is more positive than the EP+, and that the ion pump is conventional Na,K-ATPase, probably in the basolateral membrane. The latter was determined from experiments in which the ionic environment of the strial cells was controlled by perfusion of the perilymphatic space of the cochlea, in the absence of vascular circulation. While the usual EP+ was maintained by normal perfusate, replacement of Na+ by choline resulted in a negative EP, showing that Na,K-ATPase is necessary for the production of EP+. Elimination of K+ as well as Na+ from the perfusate did not change the value of the negative EP, showing that no K-ATPase is involved.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3040655

Citation

Offner, F F., et al. "Positive Endocochlear Potential: Mechanism of Production By Marginal Cells of Stria Vascularis." Hearing Research, vol. 29, no. 2-3, 1987, pp. 117-24.
Offner FF, Dallos P, Cheatham MA. Positive endocochlear potential: mechanism of production by marginal cells of stria vascularis. Hear Res. 1987;29(2-3):117-24.
Offner, F. F., Dallos, P., & Cheatham, M. A. (1987). Positive endocochlear potential: mechanism of production by marginal cells of stria vascularis. Hearing Research, 29(2-3), 117-24.
Offner FF, Dallos P, Cheatham MA. Positive Endocochlear Potential: Mechanism of Production By Marginal Cells of Stria Vascularis. Hear Res. 1987;29(2-3):117-24. PubMed PMID: 3040655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Positive endocochlear potential: mechanism of production by marginal cells of stria vascularis. AU - Offner,F F, AU - Dallos,P, AU - Cheatham,M A, PY - 1987/1/1/pubmed PY - 1987/1/1/medline PY - 1987/1/1/entrez SP - 117 EP - 24 JF - Hearing research JO - Hear Res VL - 29 IS - 2-3 N2 - The positive endocochlear potential (EP+) and high K+ concentration of the endolymph in the scala media of the mammalian cochlea are unusual. They have long been assumed to be due to a putative K-pump in the luminal membrane of the marginal cells of the stria vascularis, which were believed to have a negative internal potential. We show that the cell potential is more positive than the EP+, and that the ion pump is conventional Na,K-ATPase, probably in the basolateral membrane. The latter was determined from experiments in which the ionic environment of the strial cells was controlled by perfusion of the perilymphatic space of the cochlea, in the absence of vascular circulation. While the usual EP+ was maintained by normal perfusate, replacement of Na+ by choline resulted in a negative EP, showing that Na,K-ATPase is necessary for the production of EP+. Elimination of K+ as well as Na+ from the perfusate did not change the value of the negative EP, showing that no K-ATPase is involved. SN - 0378-5955 UR - https://www.unboundmedicine.com/medline/citation/3040655/Positive_endocochlear_potential:_mechanism_of_production_by_marginal_cells_of_stria_vascularis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-5955(87)90160-2 DB - PRIME DP - Unbound Medicine ER -