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Comparison of dnaA nucleotide sequences of Escherichia coli, Salmonella typhimurium, and Serratia marcescens.
J Bacteriol. 1987 Sep; 169(9):3976-81.JB

Abstract

The dnaA genes of Salmonella typhimurium and Serratia marcescens, which complemented the temperature-sensitive dnaA46 mutation of Escherichia coli, were cloned and sequenced. They were very homologous to the dnaA gene of E. coli. The 63 N-terminal amino acids and the 333 C-terminal amino acids of the corresponding DnaA proteins were identical. The region in between, corresponding to 71 amino acids in E. coli, exhibited a number of changes. This variable region coincided with a nonhomologous region found in the comparison of E. coli dnaA and Bacillus subtilis "dnaA" genes. The regions upstream of the genes were also homologous. The ribosome-binding area, one of the promoters, the DnaA protein-binding site, and many GATC sites (Dam methyltransferase-recognition sequence) were conserved in these three enteric bacteria.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3040670

Citation

Skovgaard, O, and F G. Hansen. "Comparison of dnaA Nucleotide Sequences of Escherichia Coli, Salmonella Typhimurium, and Serratia Marcescens." Journal of Bacteriology, vol. 169, no. 9, 1987, pp. 3976-81.
Skovgaard O, Hansen FG. Comparison of dnaA nucleotide sequences of Escherichia coli, Salmonella typhimurium, and Serratia marcescens. J Bacteriol. 1987;169(9):3976-81.
Skovgaard, O., & Hansen, F. G. (1987). Comparison of dnaA nucleotide sequences of Escherichia coli, Salmonella typhimurium, and Serratia marcescens. Journal of Bacteriology, 169(9), 3976-81.
Skovgaard O, Hansen FG. Comparison of dnaA Nucleotide Sequences of Escherichia Coli, Salmonella Typhimurium, and Serratia Marcescens. J Bacteriol. 1987;169(9):3976-81. PubMed PMID: 3040670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of dnaA nucleotide sequences of Escherichia coli, Salmonella typhimurium, and Serratia marcescens. AU - Skovgaard,O, AU - Hansen,F G, PY - 1987/9/1/pubmed PY - 1987/9/1/medline PY - 1987/9/1/entrez SP - 3976 EP - 81 JF - Journal of bacteriology JO - J Bacteriol VL - 169 IS - 9 N2 - The dnaA genes of Salmonella typhimurium and Serratia marcescens, which complemented the temperature-sensitive dnaA46 mutation of Escherichia coli, were cloned and sequenced. They were very homologous to the dnaA gene of E. coli. The 63 N-terminal amino acids and the 333 C-terminal amino acids of the corresponding DnaA proteins were identical. The region in between, corresponding to 71 amino acids in E. coli, exhibited a number of changes. This variable region coincided with a nonhomologous region found in the comparison of E. coli dnaA and Bacillus subtilis "dnaA" genes. The regions upstream of the genes were also homologous. The ribosome-binding area, one of the promoters, the DnaA protein-binding site, and many GATC sites (Dam methyltransferase-recognition sequence) were conserved in these three enteric bacteria. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/3040670/Comparison_of_dnaA_nucleotide_sequences_of_Escherichia_coli_Salmonella_typhimurium_and_Serratia_marcescens_ L2 - https://journals.asm.org/doi/10.1128/jb.169.9.3976-3981.1987?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -