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Identification of detoxification genes in imidacloprid-resistant Asian citrus psyllid (Hemiptera: Lividae) and their expression patterns under stress of eight insecticides.
Pest Manag Sci. 2019 May; 75(5):1400-1410.PM

Abstract

BACKGROUND

The Asian citrus psyllid, Diaphorina citri, is one of the major pests in citrus-growing areas around the world. The application of insecticides is the most effective method to reduce the population of D. citri. However, D. citri has developed resistance to multiple classes of insecticides. Understanding resistance mechanisms is crucial to the management of D. citri. In this study, molecular assays were performed to characterize imidacloprid resistance mechanisms.

RESULTS

Based on the D. citri transcriptome database and other known insect resistance genes, 16 cytochrome P450, eight glutathione-S-transferase and six esterase genes were selected for cloning and sequencing. The gene expression analysis of 30 detoxification genes demonstrated that the relative expression of CYP4g15, CYP303A1, CYP4C62, CYP6BD5, GSTS1 and EST-6 were moderately high (>5-fold increase) in the imidacloprid-resistant strain. Feeding of double-stranded RNA (dsRNA) reduced the expression of the six genes (46.7%-72.1%) and resulted in significant adult mortality (65.62%-82.76%). We also determined the ability of different insecticides to induce the six selected genes. The expression of CYP4C62 and GSTS1 genes were the most significantly upregulated in adults treated with all insecticides, except for chlorfenapyr. In chlorfenapyr-treated D. citri, expression of CYP4g15 and CYP303A1 were the most highly induced.

CONCLUSION

Overexpressed detoxification genes were associated with imidacloprid resistance, as confirmed by RNA interference feeding tests. The induction of the six selected genes when exposed to different insecticides supported the hypothesis that they were involved in the metabolism of the tested insecticides. © 2018 Society of Chemical Industry.

Authors+Show Affiliations

College of Agriculture, South China Agricultural University, Guangzhou, China.College of Agriculture, South China Agricultural University, Guangzhou, China.College of Agriculture, South China Agricultural University, Guangzhou, China.College of Agriculture, South China Agricultural University, Guangzhou, China.College of Agriculture, South China Agricultural University, Guangzhou, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30411865

Citation

Tian, Fajun, et al. "Identification of Detoxification Genes in Imidacloprid-resistant Asian Citrus Psyllid (Hemiptera: Lividae) and Their Expression Patterns Under Stress of Eight Insecticides." Pest Management Science, vol. 75, no. 5, 2019, pp. 1400-1410.
Tian F, Li C, Wang Z, et al. Identification of detoxification genes in imidacloprid-resistant Asian citrus psyllid (Hemiptera: Lividae) and their expression patterns under stress of eight insecticides. Pest Manag Sci. 2019;75(5):1400-1410.
Tian, F., Li, C., Wang, Z., Liu, J., & Zeng, X. (2019). Identification of detoxification genes in imidacloprid-resistant Asian citrus psyllid (Hemiptera: Lividae) and their expression patterns under stress of eight insecticides. Pest Management Science, 75(5), 1400-1410. https://doi.org/10.1002/ps.5260
Tian F, et al. Identification of Detoxification Genes in Imidacloprid-resistant Asian Citrus Psyllid (Hemiptera: Lividae) and Their Expression Patterns Under Stress of Eight Insecticides. Pest Manag Sci. 2019;75(5):1400-1410. PubMed PMID: 30411865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of detoxification genes in imidacloprid-resistant Asian citrus psyllid (Hemiptera: Lividae) and their expression patterns under stress of eight insecticides. AU - Tian,Fajun, AU - Li,Chaofeng, AU - Wang,Zhengbing, AU - Liu,Jiali, AU - Zeng,Xinnian, Y1 - 2018/12/19/ PY - 2018/05/14/received PY - 2018/11/01/revised PY - 2018/11/02/accepted PY - 2018/11/10/pubmed PY - 2019/4/19/medline PY - 2018/11/10/entrez KW - Asian citrus psyllid KW - RNA interference KW - cytochrome P450 monooxygenases KW - esterases KW - glutathione S-transferases KW - insecticides induction SP - 1400 EP - 1410 JF - Pest management science JO - Pest Manag Sci VL - 75 IS - 5 N2 - BACKGROUND: The Asian citrus psyllid, Diaphorina citri, is one of the major pests in citrus-growing areas around the world. The application of insecticides is the most effective method to reduce the population of D. citri. However, D. citri has developed resistance to multiple classes of insecticides. Understanding resistance mechanisms is crucial to the management of D. citri. In this study, molecular assays were performed to characterize imidacloprid resistance mechanisms. RESULTS: Based on the D. citri transcriptome database and other known insect resistance genes, 16 cytochrome P450, eight glutathione-S-transferase and six esterase genes were selected for cloning and sequencing. The gene expression analysis of 30 detoxification genes demonstrated that the relative expression of CYP4g15, CYP303A1, CYP4C62, CYP6BD5, GSTS1 and EST-6 were moderately high (>5-fold increase) in the imidacloprid-resistant strain. Feeding of double-stranded RNA (dsRNA) reduced the expression of the six genes (46.7%-72.1%) and resulted in significant adult mortality (65.62%-82.76%). We also determined the ability of different insecticides to induce the six selected genes. The expression of CYP4C62 and GSTS1 genes were the most significantly upregulated in adults treated with all insecticides, except for chlorfenapyr. In chlorfenapyr-treated D. citri, expression of CYP4g15 and CYP303A1 were the most highly induced. CONCLUSION: Overexpressed detoxification genes were associated with imidacloprid resistance, as confirmed by RNA interference feeding tests. The induction of the six selected genes when exposed to different insecticides supported the hypothesis that they were involved in the metabolism of the tested insecticides. © 2018 Society of Chemical Industry. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/30411865/Identification_of_detoxification_genes_in_imidacloprid_resistant_Asian_citrus_psyllid__Hemiptera:_Lividae__and_their_expression_patterns_under_stress_of_eight_insecticides_ DB - PRIME DP - Unbound Medicine ER -