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Digital droplet polymerase chain reaction analysis of common viruses in the aqueous humour of patients with Posner-Schlossman syndrome in Chinese population.
Clin Exp Ophthalmol. 2019 05; 47(4):513-520.CE

Abstract

BACKGROUND

To compare the detection results consistency of quantitative polymerase chain reaction (qPCR) and digital droplet polymerase chain reaction (ddPCR), and determine the value of ddPCR for viral detection in the aqueous humour.

METHODS

A total of 130 aqueous humour samples were collected, including 60 patients with Posner-Schlossman syndrome (PSS) in case group and 70 elderly patients with senile cataract in control group. The target nucleic acid fragments of human cytomegalovirus (HCMV), herpes simplex virus, Epstein-Barr virus and varicella zoster virus in aqueous humour were analysed by qPCR and ddPCR, respectively, for the diagnosis and curative effect monitoring of pathogen-induced PSS. Samples with inconsistent results were verified by next-generation sequencing.

RESULTS

There were 27 and 20 HCMV-positive cases detected in the case group by ddPCR and qPCR, respectively. ddPCR increased the sensitivity for the HCMV virus detection from 400 to 100 copies/mL. No other pathogens were found in this study. The results of ddPCR were consistent with that of next generation sequencing. The mean (SD) of Lg (HCMV copies/mL) detected by ddPCR and qPCR were 1.66 (1.92) and 1.10 (1.61), respectively (P < 0.001). Compared with qPCR, results of ddPCR showed better consistency with validity of clinical treatment. All patients with ddPCR-positive results had good validity on antiviral therapy, exhibiting anterior chamber inflammation remission, resolution of corneal oedema and good IOP control within 1 month.

CONCLUSIONS

HCMV was the leading cause of pathogen-induced PSS in the Chinese population. ddPCR was a promising tool for early detection, accurate diagnosis and therapeutic validity monitoring of pathogen-induced PSS. The high sensitivity of ddPCR could avoid repeated anterior chamber tap.

Authors+Show Affiliations

Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.Eye Institute, Eye and ENT Hospital, College of Medicine, Fudan University, Shanghai, China.Ophthalmology Department, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.Eye Institute, Eye and ENT Hospital, College of Medicine, Fudan University, Shanghai, China.Eye Institute, Eye and ENT Hospital, College of Medicine, Fudan University, Shanghai, China. State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Shanghai Medical College, Fudan University, Shanghai, China. Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China. Key Laboratory of Myopia, Ministry of Health, Shanghai, China.Department of Laboratory Medicine, Huashan Hospital North, Shanghai Medical College, Fudan University, Shanghai, China.Eye Institute, Eye and ENT Hospital, College of Medicine, Fudan University, Shanghai, China.Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

30414235

Citation

Cao, Guojun, et al. "Digital Droplet Polymerase Chain Reaction Analysis of Common Viruses in the Aqueous Humour of Patients With Posner-Schlossman Syndrome in Chinese Population." Clinical & Experimental Ophthalmology, vol. 47, no. 4, 2019, pp. 513-520.
Cao G, Tan C, Zhang Y, et al. Digital droplet polymerase chain reaction analysis of common viruses in the aqueous humour of patients with Posner-Schlossman syndrome in Chinese population. Clin Experiment Ophthalmol. 2019;47(4):513-520.
Cao, G., Tan, C., Zhang, Y., Kong, X., Sun, X., Ma, Y., Chen, J., & Guan, M. (2019). Digital droplet polymerase chain reaction analysis of common viruses in the aqueous humour of patients with Posner-Schlossman syndrome in Chinese population. Clinical & Experimental Ophthalmology, 47(4), 513-520. https://doi.org/10.1111/ceo.13440
Cao G, et al. Digital Droplet Polymerase Chain Reaction Analysis of Common Viruses in the Aqueous Humour of Patients With Posner-Schlossman Syndrome in Chinese Population. Clin Experiment Ophthalmol. 2019;47(4):513-520. PubMed PMID: 30414235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Digital droplet polymerase chain reaction analysis of common viruses in the aqueous humour of patients with Posner-Schlossman syndrome in Chinese population. AU - Cao,Guojun, AU - Tan,Chen, AU - Zhang,Yuyan, AU - Kong,Xiangmei, AU - Sun,Xinhuai, AU - Ma,Yanchun, AU - Chen,Junyi, AU - Guan,Ming, Y1 - 2018/12/03/ PY - 2018/09/12/received PY - 2018/10/18/revised PY - 2018/11/03/accepted PY - 2018/11/11/pubmed PY - 2020/6/6/medline PY - 2018/11/11/entrez KW - Posner-Schlossman syndrome KW - aqueous humour KW - digital droplet PCR KW - herpesvirus SP - 513 EP - 520 JF - Clinical & experimental ophthalmology JO - Clin. Experiment. Ophthalmol. VL - 47 IS - 4 N2 - BACKGROUND: To compare the detection results consistency of quantitative polymerase chain reaction (qPCR) and digital droplet polymerase chain reaction (ddPCR), and determine the value of ddPCR for viral detection in the aqueous humour. METHODS: A total of 130 aqueous humour samples were collected, including 60 patients with Posner-Schlossman syndrome (PSS) in case group and 70 elderly patients with senile cataract in control group. The target nucleic acid fragments of human cytomegalovirus (HCMV), herpes simplex virus, Epstein-Barr virus and varicella zoster virus in aqueous humour were analysed by qPCR and ddPCR, respectively, for the diagnosis and curative effect monitoring of pathogen-induced PSS. Samples with inconsistent results were verified by next-generation sequencing. RESULTS: There were 27 and 20 HCMV-positive cases detected in the case group by ddPCR and qPCR, respectively. ddPCR increased the sensitivity for the HCMV virus detection from 400 to 100 copies/mL. No other pathogens were found in this study. The results of ddPCR were consistent with that of next generation sequencing. The mean (SD) of Lg (HCMV copies/mL) detected by ddPCR and qPCR were 1.66 (1.92) and 1.10 (1.61), respectively (P < 0.001). Compared with qPCR, results of ddPCR showed better consistency with validity of clinical treatment. All patients with ddPCR-positive results had good validity on antiviral therapy, exhibiting anterior chamber inflammation remission, resolution of corneal oedema and good IOP control within 1 month. CONCLUSIONS: HCMV was the leading cause of pathogen-induced PSS in the Chinese population. ddPCR was a promising tool for early detection, accurate diagnosis and therapeutic validity monitoring of pathogen-induced PSS. The high sensitivity of ddPCR could avoid repeated anterior chamber tap. SN - 1442-9071 UR - https://www.unboundmedicine.com/medline/citation/30414235/Digital_droplet_polymerase_chain_reaction_analysis_of_common_viruses_in_the_aqueous_humour_of_patients_with_Posner_Schlossman_syndrome_in_Chinese_population_ L2 - https://doi.org/10.1111/ceo.13440 DB - PRIME DP - Unbound Medicine ER -