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Chemical characterization and evaluation of gastric antiulcer properties of the hydroethanolic extract of the stem bark of Virola elongata (Benth.) Warb.
J Ethnopharmacol. 2019 Mar 01; 231:113-124.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Virola elongata is a tree species belonging to the Myristicaceae family, distributed in the North and Midwest regions of Brazil, in the phytogeographic domain of the Amazon. The aqueous infusion or the hydroethanolic macerate of the stem bark of V. elongata are used in Brazilian and Ecuadorian indigenous folk medicine for several ethnopharmacological purposes, principally, in the treatment of stomach pain, indigestions, and gastric ulcers. This study was aimed to investigate the gastroprotective activity of this plant in order to support its popular use with scientific evidence.

MATERIALS AND METHODS

The stem bark hydroethanolic extract of the plant (HEVe) was prepared by maceration. Its qualitative and quantitative phytochemical constituents were investigated by classical colorimetric techniques, HPLC, and electrospray ionization-multiple stage fragmentation (ESI-MSn). The gastroprotective and antiulcer activity of HEVe at doses of 100, 300 and 900 mg/kg p.o. were tested using three acute (acidified ethanol, piroxicam, and in-water-restrain stress), and one chronic (acetic acid) animal ulcer models. The probable mode of action of the HEVe was evaluated by analyzing gastric acid secretion, mucus content, nitric oxide effect, and its antioxidant properties (on catalase, myeloperoxidase, and GSH content) in experimental rodents. The direct extract's activity on the growth of Helicobacter pylori was also investigated.

RESULTS

Total phenolic content in the HEVe was of 146.20 ± 1.07 mg, being flavonoids about 50% (71.79 ± 0.70 mg) of it. Comparative HPLC fingerprint analysis revealed the presence of known phenolic antiulcer compounds, such as gallic acid, catechin, and rutin. Also, methanol/water fractionation and ESI-MSn analysis of the HEVe reveals the presence of quinic acid, 3,3',4-trihydroxystilbene, juruenolid D, one catechin dimer, one C-glycosyl flavonoid, one polyketide and two neolignans as the major components of the extract. The HEVe attenuated gastric ulceration in all the different models of acute gastric ulcer, by enhancing gastroprotection through its antioxidant properties in vivo, and reducing also considerably the gastric secretion and total acidity. The HEVe also presented healing properties against the induced chronic ulceration process. On the other hand, the HEVe did not exhibit direct activity against H. pylori.

CONCLUSION

The HEVe exhibited significant gastroprotective/antiulcer effects and contain a relative high proportion of phenolic compounds, especially flavonoids, that could likely account, at least in part, for its pharmacological properties. The results justify its traditional usage and provided scientific evidence for its potential as a new herbal medicine to treat gastric ulcers.

Authors+Show Affiliations

Área de Farmacologia, Departamento de Ciências Básicas em Saúde, Universidade Federal de Mato Grosso (UFMT), Av. Fernando Correa da Costa, no. 2367, Cuiabá, MT 78060-900, Brazil.Área de Farmacologia, Departamento de Ciências Básicas em Saúde, Universidade Federal de Mato Grosso (UFMT), Av. Fernando Correa da Costa, no. 2367, Cuiabá, MT 78060-900, Brazil.Área de Farmacologia, Departamento de Ciências Básicas em Saúde, Universidade Federal de Mato Grosso (UFMT), Av. Fernando Correa da Costa, no. 2367, Cuiabá, MT 78060-900, Brazil; Curso de Farmácia, Faculdade Noroeste do Mato Grosso, Associação Juinense de Ensino Superior (AJES), Juína, MT 78320-000, Brazil.Área de Farmacologia, Departamento de Ciências Básicas em Saúde, Universidade Federal de Mato Grosso (UFMT), Av. Fernando Correa da Costa, no. 2367, Cuiabá, MT 78060-900, Brazil.Laboratório de Pesquisa de Produtos Naturais, Faculdade de Medicina, Universidade Federal de Tocantins (UFT), Palmas, TO 77020-210, Brazil.Laboratório de Pesquisa de Produtos Naturais, Faculdade de Medicina, Universidade Federal de Tocantins (UFT), Palmas, TO 77020-210, Brazil.Laboratório de Bioprospecção de Produtos Naturais. Instituto de Biociências, Universidade Estadual Paulista (UNESP) - Campus do Litoral Paulista, São Vicente, SP 11330-900, Brazil; Departamento de Química Orgânica, Instituto de Química, Universidade Estadual Paulista (UNESP) - Campus Araraquara, Araraquara, SP 14800-060, Brazil.Laboratório de Bioprospecção de Produtos Naturais. Instituto de Biociências, Universidade Estadual Paulista (UNESP) - Campus do Litoral Paulista, São Vicente, SP 11330-900, Brazil.Área de Farmacologia, Departamento de Ciências Básicas em Saúde, Universidade Federal de Mato Grosso (UFMT), Av. Fernando Correa da Costa, no. 2367, Cuiabá, MT 78060-900, Brazil.Área de Farmacologia, Departamento de Ciências Básicas em Saúde, Universidade Federal de Mato Grosso (UFMT), Av. Fernando Correa da Costa, no. 2367, Cuiabá, MT 78060-900, Brazil. Electronic address: taba@terra.com.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30415060

Citation

Almeida, Guilherme Vieira Botelho de, et al. "Chemical Characterization and Evaluation of Gastric Antiulcer Properties of the Hydroethanolic Extract of the Stem Bark of Virola Elongata (Benth.) Warb." Journal of Ethnopharmacology, vol. 231, 2019, pp. 113-124.
Almeida GVB, Arunachalam K, Balogun SO, et al. Chemical characterization and evaluation of gastric antiulcer properties of the hydroethanolic extract of the stem bark of Virola elongata (Benth.) Warb. J Ethnopharmacol. 2019;231:113-124.
Almeida, G. V. B., Arunachalam, K., Balogun, S. O., Pavan, E., Ascêncio, S. D., Soares, I. M., Zanatta, A. C., Vilegas, W., Macho, A., & Oliveira Martins, D. T. (2019). Chemical characterization and evaluation of gastric antiulcer properties of the hydroethanolic extract of the stem bark of Virola elongata (Benth.) Warb. Journal of Ethnopharmacology, 231, 113-124. https://doi.org/10.1016/j.jep.2018.11.011
Almeida GVB, et al. Chemical Characterization and Evaluation of Gastric Antiulcer Properties of the Hydroethanolic Extract of the Stem Bark of Virola Elongata (Benth.) Warb. J Ethnopharmacol. 2019 Mar 1;231:113-124. PubMed PMID: 30415060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical characterization and evaluation of gastric antiulcer properties of the hydroethanolic extract of the stem bark of Virola elongata (Benth.) Warb. AU - Almeida,Guilherme Vieira Botelho de, AU - Arunachalam,Karuppusamy, AU - Balogun,Sikiru Olaitan, AU - Pavan,Eduarda, AU - Ascêncio,Sérgio Donizeti, AU - Soares,Ilsamar Mendes, AU - Zanatta,Ana C, AU - Vilegas,Wagner, AU - Macho,Antonio, AU - Oliveira Martins,Domingos Tabajara de, Y1 - 2018/11/08/ PY - 2018/08/08/received PY - 2018/11/04/revised PY - 2018/11/05/accepted PY - 2018/11/12/pubmed PY - 2019/2/26/medline PY - 2018/11/12/entrez KW - Antioxidant KW - Antiulcer activity KW - Gastric secretion KW - Gastroprotective KW - Phenolic compounds KW - Virola elongata SP - 113 EP - 124 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 231 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Virola elongata is a tree species belonging to the Myristicaceae family, distributed in the North and Midwest regions of Brazil, in the phytogeographic domain of the Amazon. The aqueous infusion or the hydroethanolic macerate of the stem bark of V. elongata are used in Brazilian and Ecuadorian indigenous folk medicine for several ethnopharmacological purposes, principally, in the treatment of stomach pain, indigestions, and gastric ulcers. This study was aimed to investigate the gastroprotective activity of this plant in order to support its popular use with scientific evidence. MATERIALS AND METHODS: The stem bark hydroethanolic extract of the plant (HEVe) was prepared by maceration. Its qualitative and quantitative phytochemical constituents were investigated by classical colorimetric techniques, HPLC, and electrospray ionization-multiple stage fragmentation (ESI-MSn). The gastroprotective and antiulcer activity of HEVe at doses of 100, 300 and 900 mg/kg p.o. were tested using three acute (acidified ethanol, piroxicam, and in-water-restrain stress), and one chronic (acetic acid) animal ulcer models. The probable mode of action of the HEVe was evaluated by analyzing gastric acid secretion, mucus content, nitric oxide effect, and its antioxidant properties (on catalase, myeloperoxidase, and GSH content) in experimental rodents. The direct extract's activity on the growth of Helicobacter pylori was also investigated. RESULTS: Total phenolic content in the HEVe was of 146.20 ± 1.07 mg, being flavonoids about 50% (71.79 ± 0.70 mg) of it. Comparative HPLC fingerprint analysis revealed the presence of known phenolic antiulcer compounds, such as gallic acid, catechin, and rutin. Also, methanol/water fractionation and ESI-MSn analysis of the HEVe reveals the presence of quinic acid, 3,3',4-trihydroxystilbene, juruenolid D, one catechin dimer, one C-glycosyl flavonoid, one polyketide and two neolignans as the major components of the extract. The HEVe attenuated gastric ulceration in all the different models of acute gastric ulcer, by enhancing gastroprotection through its antioxidant properties in vivo, and reducing also considerably the gastric secretion and total acidity. The HEVe also presented healing properties against the induced chronic ulceration process. On the other hand, the HEVe did not exhibit direct activity against H. pylori. CONCLUSION: The HEVe exhibited significant gastroprotective/antiulcer effects and contain a relative high proportion of phenolic compounds, especially flavonoids, that could likely account, at least in part, for its pharmacological properties. The results justify its traditional usage and provided scientific evidence for its potential as a new herbal medicine to treat gastric ulcers. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/30415060/Chemical_characterization_and_evaluation_of_gastric_antiulcer_properties_of_the_hydroethanolic_extract_of_the_stem_bark_of_Virola_elongata__Benth___Warb_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(18)32933-7 DB - PRIME DP - Unbound Medicine ER -