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Photoelectrochemical determination of the activity of protein kinase A by using g-C3N4 and CdS quantum dots.
Mikrochim Acta. 2018 11 10; 185(12):541.MA

Abstract

A sensitive and selective photoelectrochemical (PEC) method is described for the detection of protein kinase A (PKA) activity based on the use of graphite-like carbon nitride (g-C3N4) and the CdS quantum dots (QDs). Firstly, a complex was synthesized from g-C3N4 and gold nanoparticles (AuNPs). It was employed as both the PEC-active material and as a support for immobilization of peptides. The latter were assembled on an ITO electrode modified with g-C3N4-AuNPs and subsequently phosphorylated by PKA in the presence of adenosine 5'-[γ-thio]triphosphate (ATP-S). Finally, CdS quantum dots (QDs) were introduced on the ITO in order to increase the PEC response of g-C3N4 based on the Cd-S binding between the QDs and thiol groups. Under the optimal conditions and a typical working voltage of -0.3 V, the method has a dynamic range that extends from 0.05 to 50 unit·mL-1, with a 0.017 unit·mL-1 lower detection limit. The method was successfully applied to the quantification of the inhibitory effect of ellagic acid on the activity of PKA, and to monitor enzyme activity in cell lysates. Graphical abstract Schematic of a sensitive and selective photoelectrochemical biosensor for the detection of protein kinase A activity. It is based on the use of graphite-like carbon nitride and CdS quantum dots.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Sui C
College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China.
Liu F
The Tumor Center of Taian City Central Hospital, Taian, Shandong, 271000, People's Republic of China.
Tang L
The Blood Transfusion Division of Taian City Central Hospital, Taian, Shandong, 271000, People's Republic of China.
Li X
College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China.
Zhou Y
College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China. zhyl@sdau.edu.cn.
Yin H
College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China.
Ai S
College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China.

MeSH

Cadmium CompoundsCyclic AMP-Dependent Protein KinasesDrug Evaluation, PreclinicalElectrochemistryElectrodesEnzyme AssaysGoldGraphiteMetal NanoparticlesNitrilesPhotochemical ProcessesProtein Kinase InhibitorsQuantum DotsSulfidesTin Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30415466

Citation

Sui, Chengji, et al. "Photoelectrochemical Determination of the Activity of Protein Kinase a By Using g-C3N4 and CdS Quantum Dots." Mikrochimica Acta, vol. 185, no. 12, 2018, p. 541.
Sui C, Liu F, Tang L, et al. Photoelectrochemical determination of the activity of protein kinase A by using g-C3N4 and CdS quantum dots. Mikrochim Acta. 2018;185(12):541.
Sui, C., Liu, F., Tang, L., Li, X., Zhou, Y., Yin, H., & Ai, S. (2018). Photoelectrochemical determination of the activity of protein kinase A by using g-C3N4 and CdS quantum dots. Mikrochimica Acta, 185(12), 541. https://doi.org/10.1007/s00604-018-3076-z
Sui C, et al. Photoelectrochemical Determination of the Activity of Protein Kinase a By Using g-C3N4 and CdS Quantum Dots. Mikrochim Acta. 2018 11 10;185(12):541. PubMed PMID: 30415466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photoelectrochemical determination of the activity of protein kinase A by using g-C3N4 and CdS quantum dots. AU - Sui,Chengji, AU - Liu,Fei, AU - Tang,Lihua, AU - Li,Xue, AU - Zhou,Yunlei, AU - Yin,Huanshun, AU - Ai,Shiyun, Y1 - 2018/11/10/ PY - 2018/07/18/received PY - 2018/10/28/accepted PY - 2018/11/12/entrez PY - 2018/11/12/pubmed PY - 2019/4/2/medline KW - Breast tissue KW - Ellagic acid KW - Gold nanoparticles KW - Graphite-like carbon nitride KW - Inhibitor screening KW - Peptide KW - Protein phosphorylation detection KW - Serum KW - Signal amplification KW - Specificity SP - 541 EP - 541 JF - Mikrochimica acta JO - Mikrochim Acta VL - 185 IS - 12 N2 - A sensitive and selective photoelectrochemical (PEC) method is described for the detection of protein kinase A (PKA) activity based on the use of graphite-like carbon nitride (g-C3N4) and the CdS quantum dots (QDs). Firstly, a complex was synthesized from g-C3N4 and gold nanoparticles (AuNPs). It was employed as both the PEC-active material and as a support for immobilization of peptides. The latter were assembled on an ITO electrode modified with g-C3N4-AuNPs and subsequently phosphorylated by PKA in the presence of adenosine 5'-[γ-thio]triphosphate (ATP-S). Finally, CdS quantum dots (QDs) were introduced on the ITO in order to increase the PEC response of g-C3N4 based on the Cd-S binding between the QDs and thiol groups. Under the optimal conditions and a typical working voltage of -0.3 V, the method has a dynamic range that extends from 0.05 to 50 unit·mL-1, with a 0.017 unit·mL-1 lower detection limit. The method was successfully applied to the quantification of the inhibitory effect of ellagic acid on the activity of PKA, and to monitor enzyme activity in cell lysates. Graphical abstract Schematic of a sensitive and selective photoelectrochemical biosensor for the detection of protein kinase A activity. It is based on the use of graphite-like carbon nitride and CdS quantum dots. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/30415466/Photoelectrochemical_determination_of_the_activity_of_protein_kinase_A_by_using_g_C3N4_and_CdS_quantum_dots_ DB - PRIME DP - Unbound Medicine ER -
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