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Characteristics of NDM-1-producing Klebsiella pneumoniae ST234 and ST1412 isolates spread in a neonatal unit.
BMC Microbiol 2018; 18(1):186BM

Abstract

BACKGROUND

The emergence of carbapenem-resistant Klebsiella pneumoniae (CR-KP) has become a significant problem worldwide and also being a major threat to children and newborns. Here we report an outbreak of NDM-1-producing K. pneumoniae in a neonatal unit.

RESULTS

Six CR-KP strains, isolated from neonates with symptoms of infection, were identified using a VITEK-2 compact system, and the clinical data were retrieved from the electronic case records. In vitro susceptibility testing with broth dilution method showed that all six K. pneumoniae isolates were resistant to carbapenems and susceptible to colistin, aminoglycosides, fluoroquinolones and tigecycline. Based on the polymerase chain reaction results, each isolate was found to be blaNDM-1 gene positive. Clonal relationships were analysed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and showed that two different PFGE patterns were formed, which belonged to sequence types ST234 and ST1412. Plasmids carrying blaNDM-1 were successfully transferred from four of the six isolates to an Escherichia coli recipient through conjugative assays. S1-PFGE and Southern blot hybridization showed that four NDM-1-producing K. pneumoniae were clonal and carried blaNDM-1 on the same plasmid. The outbreak was effectively controlled by reducing the potential infection sources. All the patients were successfully treated and recovered after receiving an increased dose of carbapenems. Although the source of this outbreak was not clear, comprehensive measures were carried out and the outbreak was effectively controlled.

CONCLUSIONS

ST234 and ST1412 of NDM-1-producing Klebsiella pneumoniae are the resistant clone spread in the neonatal unit, comprehensive infection control measures and optimized carbapenem therapy played an important role in controlling this NDM-1-producing K. pneumoniae outbreak.

Authors+Show Affiliations

Department of Laboratory Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, People's Republic of China. National Key Clinical Department of Laboratory Medicine, Nanjing, 210029, People's Republic of China.Department of Laboratory Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, People's Republic of China. National Key Clinical Department of Laboratory Medicine, Nanjing, 210029, People's Republic of China.Department of Laboratory Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, People's Republic of China. National Key Clinical Department of Laboratory Medicine, Nanjing, 210029, People's Republic of China.Department of Laboratory Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, People's Republic of China. National Key Clinical Department of Laboratory Medicine, Nanjing, 210029, People's Republic of China.Department of Laboratory Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, People's Republic of China. National Key Clinical Department of Laboratory Medicine, Nanjing, 210029, People's Republic of China.Department of Laboratory Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, People's Republic of China. liugenyan@jsph.org.cn. National Key Clinical Department of Laboratory Medicine, Nanjing, 210029, People's Republic of China. liugenyan@jsph.org.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30428842

Citation

Huang, Xu, et al. "Characteristics of NDM-1-producing Klebsiella Pneumoniae ST234 and ST1412 Isolates Spread in a Neonatal Unit." BMC Microbiology, vol. 18, no. 1, 2018, p. 186.
Huang X, Cheng X, Sun P, et al. Characteristics of NDM-1-producing Klebsiella pneumoniae ST234 and ST1412 isolates spread in a neonatal unit. BMC Microbiol. 2018;18(1):186.
Huang, X., Cheng, X., Sun, P., Tang, C., Ni, F., & Liu, G. (2018). Characteristics of NDM-1-producing Klebsiella pneumoniae ST234 and ST1412 isolates spread in a neonatal unit. BMC Microbiology, 18(1), p. 186. doi:10.1186/s12866-018-1334-1.
Huang X, et al. Characteristics of NDM-1-producing Klebsiella Pneumoniae ST234 and ST1412 Isolates Spread in a Neonatal Unit. BMC Microbiol. 2018 11 14;18(1):186. PubMed PMID: 30428842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristics of NDM-1-producing Klebsiella pneumoniae ST234 and ST1412 isolates spread in a neonatal unit. AU - Huang,Xu, AU - Cheng,Xiangjun, AU - Sun,Pengfei, AU - Tang,Chenjie, AU - Ni,Fang, AU - Liu,Genyan, Y1 - 2018/11/14/ PY - 2018/04/27/received PY - 2018/11/01/accepted PY - 2018/11/16/entrez PY - 2018/11/16/pubmed PY - 2019/8/16/medline KW - Multidrug-Resistant, carbapenem-resistant Klebsiella pneumoniae KW - Neonate KW - Outbreak KW - bla NDM-1 SP - 186 EP - 186 JF - BMC microbiology JO - BMC Microbiol. VL - 18 IS - 1 N2 - BACKGROUND: The emergence of carbapenem-resistant Klebsiella pneumoniae (CR-KP) has become a significant problem worldwide and also being a major threat to children and newborns. Here we report an outbreak of NDM-1-producing K. pneumoniae in a neonatal unit. RESULTS: Six CR-KP strains, isolated from neonates with symptoms of infection, were identified using a VITEK-2 compact system, and the clinical data were retrieved from the electronic case records. In vitro susceptibility testing with broth dilution method showed that all six K. pneumoniae isolates were resistant to carbapenems and susceptible to colistin, aminoglycosides, fluoroquinolones and tigecycline. Based on the polymerase chain reaction results, each isolate was found to be blaNDM-1 gene positive. Clonal relationships were analysed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and showed that two different PFGE patterns were formed, which belonged to sequence types ST234 and ST1412. Plasmids carrying blaNDM-1 were successfully transferred from four of the six isolates to an Escherichia coli recipient through conjugative assays. S1-PFGE and Southern blot hybridization showed that four NDM-1-producing K. pneumoniae were clonal and carried blaNDM-1 on the same plasmid. The outbreak was effectively controlled by reducing the potential infection sources. All the patients were successfully treated and recovered after receiving an increased dose of carbapenems. Although the source of this outbreak was not clear, comprehensive measures were carried out and the outbreak was effectively controlled. CONCLUSIONS: ST234 and ST1412 of NDM-1-producing Klebsiella pneumoniae are the resistant clone spread in the neonatal unit, comprehensive infection control measures and optimized carbapenem therapy played an important role in controlling this NDM-1-producing K. pneumoniae outbreak. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/30428842/Characteristics_of_NDM_1_producing_Klebsiella_pneumoniae_ST234_and_ST1412_isolates_spread_in_a_neonatal_unit_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-018-1334-1 DB - PRIME DP - Unbound Medicine ER -