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Preparation of a poly(ethyleneimine) embedded phenyl stationary phase for mixed-mode liquid chromatography.
Anal Chim Acta 2018; 1042:165-173AC

Abstract

A poly(ethyleneimine) embedded phenyl mixed-mode stationary phase was prepared through epoxide ring opening reaction. Elemental analysis (EA), Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA) were used to characterize whether functional groups have been modified on the surface of silica successfully. The researches of chromatographic performance for designed column were divided to four parts. The retention behavior under reversed-phase mode was studied using polycyclic aromatic hydrocarbons (PAHs), positional isomers and alkylbenzenes. The retention characteristic under hydrophilic interaction mode was studied by nucleosides, nucleobases and flavonoids. The chromatographic performance of anion exchange mode was evaluated by benzoic acids and phenols. Due to a high content of amino and phenyl groups on the stationary phase, aromatic amines were chosen for the evaluation of retention mechanisms of benzene ring attraction and amino repulsion. The investigations about effects of mobile phase constitution and pH on retention demonstrated the mixed-mode retention mechanisms of the column. Furthermore, due to amino selectivity of the column, a method for the rapid separation and determination of phenylenediamines in hair dye was established. And both linear correlation coefficients of p-diaminobenzene and m-diaminobenzene in concentration range from 0.1 to 30 μg mL-1 were over 0.999. In conclude, the prepared mixed-mode stationary phase could realize various separation modes by adjusting chromatographic conditions, and it had the potential for the rapid separation and determination of aniline compounds in various complex samples.

Authors+Show Affiliations

School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Nursing, Southwest Medical University, Luzhou, Sichuan, 646000, China. Electronic address: 604407569@qq.com.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China. Electronic address: wlj@swmu.edu.cn.School of Pharmacy, Chongqing University, Chongqing, 401331, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30428983

Citation

Ren, Xiujun, et al. "Preparation of a Poly(ethyleneimine) Embedded Phenyl Stationary Phase for Mixed-mode Liquid Chromatography." Analytica Chimica Acta, vol. 1042, 2018, pp. 165-173.
Ren X, Hu C, Gao D, et al. Preparation of a poly(ethyleneimine) embedded phenyl stationary phase for mixed-mode liquid chromatography. Anal Chim Acta. 2018;1042:165-173.
Ren, X., Hu, C., Gao, D., Fu, Q., Zhang, K., Zu, F., ... Xia, Z. (2018). Preparation of a poly(ethyleneimine) embedded phenyl stationary phase for mixed-mode liquid chromatography. Analytica Chimica Acta, 1042, pp. 165-173. doi:10.1016/j.aca.2018.09.049.
Ren X, et al. Preparation of a Poly(ethyleneimine) Embedded Phenyl Stationary Phase for Mixed-mode Liquid Chromatography. Anal Chim Acta. 2018 Dec 26;1042:165-173. PubMed PMID: 30428983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of a poly(ethyleneimine) embedded phenyl stationary phase for mixed-mode liquid chromatography. AU - Ren,Xiujun, AU - Hu,Chengxia, AU - Gao,Die, AU - Fu,Qifeng, AU - Zhang,Kailian, AU - Zu,Fengjiao, AU - Zeng,Jing, AU - Wang,Lujun, AU - Xia,Zhining, Y1 - 2018/09/23/ PY - 2018/07/22/received PY - 2018/09/13/revised PY - 2018/09/20/accepted PY - 2018/11/16/entrez PY - 2018/11/16/pubmed PY - 2018/11/16/medline KW - Hair dye KW - Mixed-mode chromatography KW - Poly(ethyleneimine) KW - Reversed phase/hydrophilic interaction/ion exchange KW - Stationary phase SP - 165 EP - 173 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 1042 N2 - A poly(ethyleneimine) embedded phenyl mixed-mode stationary phase was prepared through epoxide ring opening reaction. Elemental analysis (EA), Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA) were used to characterize whether functional groups have been modified on the surface of silica successfully. The researches of chromatographic performance for designed column were divided to four parts. The retention behavior under reversed-phase mode was studied using polycyclic aromatic hydrocarbons (PAHs), positional isomers and alkylbenzenes. The retention characteristic under hydrophilic interaction mode was studied by nucleosides, nucleobases and flavonoids. The chromatographic performance of anion exchange mode was evaluated by benzoic acids and phenols. Due to a high content of amino and phenyl groups on the stationary phase, aromatic amines were chosen for the evaluation of retention mechanisms of benzene ring attraction and amino repulsion. The investigations about effects of mobile phase constitution and pH on retention demonstrated the mixed-mode retention mechanisms of the column. Furthermore, due to amino selectivity of the column, a method for the rapid separation and determination of phenylenediamines in hair dye was established. And both linear correlation coefficients of p-diaminobenzene and m-diaminobenzene in concentration range from 0.1 to 30 μg mL-1 were over 0.999. In conclude, the prepared mixed-mode stationary phase could realize various separation modes by adjusting chromatographic conditions, and it had the potential for the rapid separation and determination of aniline compounds in various complex samples. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/30428983/Preparation_of_a_poly_ethyleneimine__embedded_phenyl_stationary_phase_for_mixed_mode_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(18)31142-5 DB - PRIME DP - Unbound Medicine ER -