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The complete genome sequence of a second alphabaculovirus from the true armyworm, Mythimna unipuncta: implications for baculovirus phylogeny and host specificity.
Virus Genes 2019; 55(1):104-116VG

Abstract

The Mythimna unipuncta nucleopolyhedrovirus isolate KY310 (MyunNPV-KY310) is an alphabaculovirus isolated from a true armyworm (Mythimna unipuncta) population in Kentucky, USA. Occlusion bodies of this virus were examined by electron microscopy and the genome sequence was determined by 454 pyrosequencing. MyunNPV-KY310 occlusion bodies consisted of irregular polyhedra measuring 0.8-1.8 µm in diameter and containing multiple virions, with one to six nucleocapsids per virion. The genome sequence was determined to be 156,647 bp with a nucleotide distribution of 43.9% G+C. 152 ORFs and six homologous repeat (hr) regions were annotated for the sequence, including the 38 core genes of family Baculoviridae and an additional group of 26 conserved alphabaculovirus genes. BLAST queries and phylogenetic inference confirmed that MyunNPV-KY310 is most closely related to the alphabaculovirus Leucania separata nucleopolyhedrovirus isolate AH1, which infects Mythimna separata. In contrast, MyunNPV-KY310 did not exhibit a close relationship with Mythimna unipuncta nucleopolyhedrovirus isolate #7, an alphabaculovirus from the same host species. MyunNPV-KY310 lacks the gp64 envelope glycoprotein, which is a characteristic of group II alphabaculoviruses. However, this virus and five other alphabaculoviruses lacking gp64 are placed outside the group I and group II clades in core gene phylogenies, further demonstrating that viruses of genus Alphabaculovirus do not occur in two monophyletic clades. Potential instances of MyunNPV-KY310 ORFs arising by horizontal transfer were detected. Although there are now genome sequences of four different baculoviruses from M. unipuncta, comparison of their genome sequences provides little insight into the genetic basis for their host specificity.

Authors+Show Affiliations

Invasive Insect Biocontrol and Behavior Laboratory, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD, 20705, USA. Robert.L.Harrison@ars.usda.gov.Electron and Confocal Microscopy Unit, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD, 20705, USA.Electron and Confocal Microscopy Unit, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD, 20705, USA.Summerland Research and Development Centre, Agriculture and Agri-Food Canada, Summerland, BC, V0H 1Z0, Canada.Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK, S7N 0X2, Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30430308

Citation

Harrison, Robert L., et al. "The Complete Genome Sequence of a Second Alphabaculovirus From the True Armyworm, Mythimna Unipuncta: Implications for Baculovirus Phylogeny and Host Specificity." Virus Genes, vol. 55, no. 1, 2019, pp. 104-116.
Harrison RL, Mowery JD, Bauchan GR, et al. The complete genome sequence of a second alphabaculovirus from the true armyworm, Mythimna unipuncta: implications for baculovirus phylogeny and host specificity. Virus Genes. 2019;55(1):104-116.
Harrison, R. L., Mowery, J. D., Bauchan, G. R., Theilmann, D. A., & Erlandson, M. A. (2019). The complete genome sequence of a second alphabaculovirus from the true armyworm, Mythimna unipuncta: implications for baculovirus phylogeny and host specificity. Virus Genes, 55(1), pp. 104-116. doi:10.1007/s11262-018-1615-7.
Harrison RL, et al. The Complete Genome Sequence of a Second Alphabaculovirus From the True Armyworm, Mythimna Unipuncta: Implications for Baculovirus Phylogeny and Host Specificity. Virus Genes. 2019;55(1):104-116. PubMed PMID: 30430308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The complete genome sequence of a second alphabaculovirus from the true armyworm, Mythimna unipuncta: implications for baculovirus phylogeny and host specificity. AU - Harrison,Robert L, AU - Mowery,Joseph D, AU - Bauchan,Gary R, AU - Theilmann,David A, AU - Erlandson,Martin A, Y1 - 2018/11/14/ PY - 2018/08/30/received PY - 2018/11/09/accepted PY - 2018/11/16/pubmed PY - 2019/2/23/medline PY - 2018/11/16/entrez KW - Alphabaculovirus KW - Baculovirus KW - Mythimna unipuncta KW - MyunNPV KW - Nucleopolyhedrovirus KW - True armyworm SP - 104 EP - 116 JF - Virus genes JO - Virus Genes VL - 55 IS - 1 N2 - The Mythimna unipuncta nucleopolyhedrovirus isolate KY310 (MyunNPV-KY310) is an alphabaculovirus isolated from a true armyworm (Mythimna unipuncta) population in Kentucky, USA. Occlusion bodies of this virus were examined by electron microscopy and the genome sequence was determined by 454 pyrosequencing. MyunNPV-KY310 occlusion bodies consisted of irregular polyhedra measuring 0.8-1.8 µm in diameter and containing multiple virions, with one to six nucleocapsids per virion. The genome sequence was determined to be 156,647 bp with a nucleotide distribution of 43.9% G+C. 152 ORFs and six homologous repeat (hr) regions were annotated for the sequence, including the 38 core genes of family Baculoviridae and an additional group of 26 conserved alphabaculovirus genes. BLAST queries and phylogenetic inference confirmed that MyunNPV-KY310 is most closely related to the alphabaculovirus Leucania separata nucleopolyhedrovirus isolate AH1, which infects Mythimna separata. In contrast, MyunNPV-KY310 did not exhibit a close relationship with Mythimna unipuncta nucleopolyhedrovirus isolate #7, an alphabaculovirus from the same host species. MyunNPV-KY310 lacks the gp64 envelope glycoprotein, which is a characteristic of group II alphabaculoviruses. However, this virus and five other alphabaculoviruses lacking gp64 are placed outside the group I and group II clades in core gene phylogenies, further demonstrating that viruses of genus Alphabaculovirus do not occur in two monophyletic clades. Potential instances of MyunNPV-KY310 ORFs arising by horizontal transfer were detected. Although there are now genome sequences of four different baculoviruses from M. unipuncta, comparison of their genome sequences provides little insight into the genetic basis for their host specificity. SN - 1572-994X UR - https://www.unboundmedicine.com/medline/citation/30430308/The_complete_genome_sequence_of_a_second_alphabaculovirus_from_the_true_armyworm_Mythimna_unipuncta:_implications_for_baculovirus_phylogeny_and_host_specificity_ L2 - https://doi.org/10.1007/s11262-018-1615-7 DB - PRIME DP - Unbound Medicine ER -