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Effects of pH Variability on Peracetic Acid Reduction of Human Norovirus GI, GII RNA, and Infectivity Plus RNA Reduction of Selected Surrogates.
Food Environ Virol. 2019 03; 11(1):76-89.FE

Abstract

With increasing interest in peracetic acid (PAA) as a disinfectant in water treatment processes, this study determined PAA treatment effects on human noroviruses (hNoVs) genotype I (GI) and genotype II (GII) as well as effects on bacteriophage MS2 and murine norovirus (MNV) in relation to pH. Across all pH conditions, PAA achieved between 0.2 and 2.5 log10 reduction of hNoVs over 120 min contact time in buffer solution as measured by reverse transcription-qPCR (RT-qPCR). The PAA treatments produced similar RT-qPCR reductions of MS2 and MNV, in the range of 0.2-2.7 log10. Infectivity assays achieved > 4 log10 reduction of both MS2 and MNV in buffer solution after 120 min contact time. Comparing PAA activity across varying pH, disinfection at pH 8.5, in general, resulted in less reduction of infectivity and molecular signals compared to pH conditions of 6.5 and 7.5. This difference was most pronounced for reductions in infectivity of MNV and MS2, with as much as 2.7 log10 less reduction at pH 8.5 relative to lower pH conditions. This study revealed that PAA was an effective disinfectant for treatment of hNoV GI and GII, MS2 and MNV, with greatest virus reduction observed for MS2 and MNV infectivity. RT-qPCR reductions of MS2 and MNV were lower than concurrent MS2 and MNV infectivity reductions, suggesting that observed hNoV RT-qPCR reductions may underestimate reductions in hNoV infectivity achieved by PAA. Although virus disinfection by PAA occurred at all evaluated pH levels, PAA is most effective at pH 6.5-7.5.

Authors+Show Affiliations

Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.JHU/Stantec Alliance, Johns Hopkins University, Baltimore, MD, USA.Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA. JHU/Stantec Alliance, Johns Hopkins University, Baltimore, MD, USA. MWH-Stantec, Pasadena, CA, USA.Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA. kschwab1@jhu.edu. JHU/Stantec Alliance, Johns Hopkins University, Baltimore, MD, USA. kschwab1@jhu.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30430442

Citation

Dunkin, Nathan, et al. "Effects of pH Variability On Peracetic Acid Reduction of Human Norovirus GI, GII RNA, and Infectivity Plus RNA Reduction of Selected Surrogates." Food and Environmental Virology, vol. 11, no. 1, 2019, pp. 76-89.
Dunkin N, Coulter C, Weng S, et al. Effects of pH Variability on Peracetic Acid Reduction of Human Norovirus GI, GII RNA, and Infectivity Plus RNA Reduction of Selected Surrogates. Food Environ Virol. 2019;11(1):76-89.
Dunkin, N., Coulter, C., Weng, S., Jacangelo, J. G., & Schwab, K. J. (2019). Effects of pH Variability on Peracetic Acid Reduction of Human Norovirus GI, GII RNA, and Infectivity Plus RNA Reduction of Selected Surrogates. Food and Environmental Virology, 11(1), 76-89. https://doi.org/10.1007/s12560-018-9359-z
Dunkin N, et al. Effects of pH Variability On Peracetic Acid Reduction of Human Norovirus GI, GII RNA, and Infectivity Plus RNA Reduction of Selected Surrogates. Food Environ Virol. 2019;11(1):76-89. PubMed PMID: 30430442.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of pH Variability on Peracetic Acid Reduction of Human Norovirus GI, GII RNA, and Infectivity Plus RNA Reduction of Selected Surrogates. AU - Dunkin,Nathan, AU - Coulter,Caroline, AU - Weng,ShihChi, AU - Jacangelo,Joseph G, AU - Schwab,Kellogg J, Y1 - 2018/11/15/ PY - 2018/07/28/received PY - 2018/11/07/accepted PY - 2018/11/16/pubmed PY - 2019/8/2/medline PY - 2018/11/16/entrez KW - Disinfection KW - Human norovirus KW - MS2 bacteriophage KW - Mouse norovirus KW - Peracetic acid KW - pH SP - 76 EP - 89 JF - Food and environmental virology JO - Food Environ Virol VL - 11 IS - 1 N2 - With increasing interest in peracetic acid (PAA) as a disinfectant in water treatment processes, this study determined PAA treatment effects on human noroviruses (hNoVs) genotype I (GI) and genotype II (GII) as well as effects on bacteriophage MS2 and murine norovirus (MNV) in relation to pH. Across all pH conditions, PAA achieved between 0.2 and 2.5 log10 reduction of hNoVs over 120 min contact time in buffer solution as measured by reverse transcription-qPCR (RT-qPCR). The PAA treatments produced similar RT-qPCR reductions of MS2 and MNV, in the range of 0.2-2.7 log10. Infectivity assays achieved > 4 log10 reduction of both MS2 and MNV in buffer solution after 120 min contact time. Comparing PAA activity across varying pH, disinfection at pH 8.5, in general, resulted in less reduction of infectivity and molecular signals compared to pH conditions of 6.5 and 7.5. This difference was most pronounced for reductions in infectivity of MNV and MS2, with as much as 2.7 log10 less reduction at pH 8.5 relative to lower pH conditions. This study revealed that PAA was an effective disinfectant for treatment of hNoV GI and GII, MS2 and MNV, with greatest virus reduction observed for MS2 and MNV infectivity. RT-qPCR reductions of MS2 and MNV were lower than concurrent MS2 and MNV infectivity reductions, suggesting that observed hNoV RT-qPCR reductions may underestimate reductions in hNoV infectivity achieved by PAA. Although virus disinfection by PAA occurred at all evaluated pH levels, PAA is most effective at pH 6.5-7.5. SN - 1867-0342 UR - https://www.unboundmedicine.com/medline/citation/30430442/Effects_of_pH_Variability_on_Peracetic_Acid_Reduction_of_Human_Norovirus_GI_GII_RNA_and_Infectivity_Plus_RNA_Reduction_of_Selected_Surrogates_ DB - PRIME DP - Unbound Medicine ER -