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Identification and confirmation of diuretics and masking agents in urine by turbulent flow online solid-phase extraction coupled with liquid chromatography-triple quadrupole mass spectrometry for doping control.
J Chromatogr A. 2018 Dec 07; 1579:31-40.JC

Abstract

Diuretics can be misused to force diuresis to achieve weight loss or to mask the intake of a prohibited substance and are therefore prohibited by the World Anti-Doping Agency (WADA). For similar reasons other masking agents (vaptans, probenecid, etc.) are also prohibited by the WADA. The currently employed methods to detect diuretics in urine use extraction or dilute-and-shoot, combined with 1D- liquid chromatography (LC) high resolution mass spectrometry (MS) or LC-triple quadrupole MS. Dilute-and-shoot methods save time and work, but these methods encounter some problems (e.g., peak drift and matrix effect). Therefore, a 2D-LC-MS/MS application was developed, validated and evaluated as an alternative. The effect of a turbulent flow rate was studied by loading samples under different conditions and the turbulent flow rate was found to be more effective in removing matrix interferences. A correlation with the specific gravity was observed. A turbulent flow online solid phase extraction (SPE) method combined with LC-MS/MS for the detection of 50 diuretics and masking agents was developed and validated for identification purposes. This method combines the advantages of dilute-and-shoot while solving the issues of matrix effect and retention time shift. Furthermore, the presented method is compliant with WADA's identification criteria and can hence be used for screening and/or confirmation.

Authors+Show Affiliations

Doping Control Laboratory (DoCoLab), Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Technologiepark 30B, B-9052 Zwijnaarde, Belgium. Electronic address: Laurie.DeWilde@UGent.be.Doping Control Laboratory (DoCoLab), Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Technologiepark 30B, B-9052 Zwijnaarde, Belgium.Doping Control Laboratory (DoCoLab), Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Technologiepark 30B, B-9052 Zwijnaarde, Belgium.Doping Control Laboratory (DoCoLab), Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Technologiepark 30B, B-9052 Zwijnaarde, Belgium.Doping Control Laboratory (DoCoLab), Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Technologiepark 30B, B-9052 Zwijnaarde, Belgium.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30430987

Citation

De Wilde, Laurie, et al. "Identification and Confirmation of Diuretics and Masking Agents in Urine By Turbulent Flow Online Solid-phase Extraction Coupled With Liquid Chromatography-triple Quadrupole Mass Spectrometry for Doping Control." Journal of Chromatography. A, vol. 1579, 2018, pp. 31-40.
De Wilde L, Roels K, Polet M, et al. Identification and confirmation of diuretics and masking agents in urine by turbulent flow online solid-phase extraction coupled with liquid chromatography-triple quadrupole mass spectrometry for doping control. J Chromatogr A. 2018;1579:31-40.
De Wilde, L., Roels, K., Polet, M., Van Eenoo, P., & Deventer, K. (2018). Identification and confirmation of diuretics and masking agents in urine by turbulent flow online solid-phase extraction coupled with liquid chromatography-triple quadrupole mass spectrometry for doping control. Journal of Chromatography. A, 1579, 31-40. https://doi.org/10.1016/j.chroma.2018.10.032
De Wilde L, et al. Identification and Confirmation of Diuretics and Masking Agents in Urine By Turbulent Flow Online Solid-phase Extraction Coupled With Liquid Chromatography-triple Quadrupole Mass Spectrometry for Doping Control. J Chromatogr A. 2018 Dec 7;1579:31-40. PubMed PMID: 30430987.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and confirmation of diuretics and masking agents in urine by turbulent flow online solid-phase extraction coupled with liquid chromatography-triple quadrupole mass spectrometry for doping control. AU - De Wilde,Laurie, AU - Roels,Kris, AU - Polet,Michaël, AU - Van Eenoo,Peter, AU - Deventer,Koen, Y1 - 2018/10/17/ PY - 2018/07/20/received PY - 2018/10/09/revised PY - 2018/10/16/accepted PY - 2018/11/16/entrez PY - 2018/11/16/pubmed PY - 2018/12/12/medline KW - Diuretics KW - Doping KW - Mass spectrometry KW - Turbulent flow online solid phase extraction KW - Two-dimensional liquid chromatography KW - Urine SP - 31 EP - 40 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1579 N2 - Diuretics can be misused to force diuresis to achieve weight loss or to mask the intake of a prohibited substance and are therefore prohibited by the World Anti-Doping Agency (WADA). For similar reasons other masking agents (vaptans, probenecid, etc.) are also prohibited by the WADA. The currently employed methods to detect diuretics in urine use extraction or dilute-and-shoot, combined with 1D- liquid chromatography (LC) high resolution mass spectrometry (MS) or LC-triple quadrupole MS. Dilute-and-shoot methods save time and work, but these methods encounter some problems (e.g., peak drift and matrix effect). Therefore, a 2D-LC-MS/MS application was developed, validated and evaluated as an alternative. The effect of a turbulent flow rate was studied by loading samples under different conditions and the turbulent flow rate was found to be more effective in removing matrix interferences. A correlation with the specific gravity was observed. A turbulent flow online solid phase extraction (SPE) method combined with LC-MS/MS for the detection of 50 diuretics and masking agents was developed and validated for identification purposes. This method combines the advantages of dilute-and-shoot while solving the issues of matrix effect and retention time shift. Furthermore, the presented method is compliant with WADA's identification criteria and can hence be used for screening and/or confirmation. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/30430987/Identification_and_confirmation_of_diuretics_and_masking_agents_in_urine_by_turbulent_flow_online_solid_phase_extraction_coupled_with_liquid_chromatography_triple_quadrupole_mass_spectrometry_for_doping_control_ DB - PRIME DP - Unbound Medicine ER -