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Influence of hybrid polymeric nanoparticle/thermosensitive hydrogels systems on formulation tracking and in vitro artificial membrane permeation: A promising system for skin drug-delivery.

Abstract

In recent years, the development of hybrid drug delivery systems, such as hydrogels and nanoparticles, has gained considerable attention as new formulations for skin-delivery. Meanwhile, transdermal diffusion synthetic membranes have been used to assess skin permeability to these systems, providing key insights into the relationships between drug and nanoformulations. In this study, benzocaine-loaded poly-ε-caprolactone nanoparticles (BZC:NPs) were synthesized, characterized and incorporated into Poloxamer 407-based hydrogel (PL407). Benzocaine (BZC) was used as a drug model since has been commonly applied as a topical pain reliever in the last years. Hence, we developed a hybrid polymeric nanoparticle/thermosensitive hydrogels system and evaluated the in vitro permeation of the BZC, as well as nanoformulation tracking in an artificial membrane. In vitro permeation study was conducted in a vertical diffusion cell system using a Strat-M® membrane model. BZC:NPs were prepared by coprecipitation method and their physicochemical stability measured before incorporating into the thermosensitive hydrogel. Also, viscosity measurements and sol-gel transition temperature were performed by rheological analysis. Different techniques, including microscopy, were used to tracking the nanoparticles on both receptor medium and synthetic membranes. Results showed high BZC encapsulation efficiency into NPs (93%) and good physicochemical stability before and after hydrogel incorporation. BZC in vitro permeation kinetics from NPs-loaded Poloxamer 407-based hydrogel presented slower permeation profile compared with the BZC: Poloxamer 407-based hydrogel. Also, NPs were observed into the diffusion cells receptor compartment after the in vitro permeation study. These results contribute to a better understanding the interaction between hydrogels, nanoparticles and synthetic membrane, as well as open perspectives for the development of new drug delivery systems for skin.

Authors+Show Affiliations

São Paulo State University (UNESP), Department of Physics and Chemistry, School of Engineering, Ilha Solteira, SP, Brazil. Electronic address: renato.grillo@unesp.br.Human and Natural Sciences Center, Federal University of ABC (UFABC), Santo André, SP, Brazil.Human and Natural Sciences Center, Federal University of ABC (UFABC), Santo André, SP, Brazil.Human and Natural Sciences Center, Federal University of ABC (UFABC), Santo André, SP, Brazil.São Paulo State University (UNESP), Department of Environmental Engineering, Sorocaba, SP, Brazil.Department of Biochemistry and Tissue Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.Human and Natural Sciences Center, Federal University of ABC (UFABC), Santo André, SP, Brazil. Electronic address: daniele.araujo@ufabc.edu.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30439638

Citation

Grillo, Renato, et al. "Influence of Hybrid Polymeric Nanoparticle/thermosensitive Hydrogels Systems On Formulation Tracking and in Vitro Artificial Membrane Permeation: a Promising System for Skin Drug-delivery." Colloids and Surfaces. B, Biointerfaces, vol. 174, 2019, pp. 56-62.
Grillo R, Dias FV, Querobino SM, et al. Influence of hybrid polymeric nanoparticle/thermosensitive hydrogels systems on formulation tracking and in vitro artificial membrane permeation: A promising system for skin drug-delivery. Colloids Surf B Biointerfaces. 2019;174:56-62.
Grillo, R., Dias, F. V., Querobino, S. M., Alberto-Silva, C., Fraceto, L. F., de Paula, E., & de Araujo, D. R. (2019). Influence of hybrid polymeric nanoparticle/thermosensitive hydrogels systems on formulation tracking and in vitro artificial membrane permeation: A promising system for skin drug-delivery. Colloids and Surfaces. B, Biointerfaces, 174, pp. 56-62. doi:10.1016/j.colsurfb.2018.10.063.
Grillo R, et al. Influence of Hybrid Polymeric Nanoparticle/thermosensitive Hydrogels Systems On Formulation Tracking and in Vitro Artificial Membrane Permeation: a Promising System for Skin Drug-delivery. Colloids Surf B Biointerfaces. 2019 Feb 1;174:56-62. PubMed PMID: 30439638.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of hybrid polymeric nanoparticle/thermosensitive hydrogels systems on formulation tracking and in vitro artificial membrane permeation: A promising system for skin drug-delivery. AU - Grillo,Renato, AU - Dias,Fabiana V, AU - Querobino,Samyr M, AU - Alberto-Silva,Carlos, AU - Fraceto,Leonardo F, AU - de Paula,Eneida, AU - de Araujo,Daniele R, Y1 - 2018/10/26/ PY - 2018/05/18/received PY - 2018/10/17/revised PY - 2018/10/18/accepted PY - 2018/11/16/pubmed PY - 2019/6/14/medline PY - 2018/11/16/entrez KW - Controlled release KW - Hybrid systems KW - Hydrogels KW - Nanoparticles KW - Permeation study KW - Synthetic membrane SP - 56 EP - 62 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 174 N2 - In recent years, the development of hybrid drug delivery systems, such as hydrogels and nanoparticles, has gained considerable attention as new formulations for skin-delivery. Meanwhile, transdermal diffusion synthetic membranes have been used to assess skin permeability to these systems, providing key insights into the relationships between drug and nanoformulations. In this study, benzocaine-loaded poly-ε-caprolactone nanoparticles (BZC:NPs) were synthesized, characterized and incorporated into Poloxamer 407-based hydrogel (PL407). Benzocaine (BZC) was used as a drug model since has been commonly applied as a topical pain reliever in the last years. Hence, we developed a hybrid polymeric nanoparticle/thermosensitive hydrogels system and evaluated the in vitro permeation of the BZC, as well as nanoformulation tracking in an artificial membrane. In vitro permeation study was conducted in a vertical diffusion cell system using a Strat-M® membrane model. BZC:NPs were prepared by coprecipitation method and their physicochemical stability measured before incorporating into the thermosensitive hydrogel. Also, viscosity measurements and sol-gel transition temperature were performed by rheological analysis. Different techniques, including microscopy, were used to tracking the nanoparticles on both receptor medium and synthetic membranes. Results showed high BZC encapsulation efficiency into NPs (93%) and good physicochemical stability before and after hydrogel incorporation. BZC in vitro permeation kinetics from NPs-loaded Poloxamer 407-based hydrogel presented slower permeation profile compared with the BZC: Poloxamer 407-based hydrogel. Also, NPs were observed into the diffusion cells receptor compartment after the in vitro permeation study. These results contribute to a better understanding the interaction between hydrogels, nanoparticles and synthetic membrane, as well as open perspectives for the development of new drug delivery systems for skin. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/30439638/Influence_of_hybrid_polymeric_nanoparticle/thermosensitive_hydrogels_systems_on_formulation_tracking_and_in_vitro_artificial_membrane_permeation:_A_promising_system_for_skin_drug_delivery_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(18)30747-1 DB - PRIME DP - Unbound Medicine ER -