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A Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativa.
Front Plant Sci. 2018; 9:1576.FP

Abstract

There have been strong interests in producing unusual fatty acids in oilseed crops to provide renewable industrial feedstock. Results are so far largely disappointing since much lower amounts of such fatty acids accumulate in genetically engineered seeds than in their original natural sources. It has been suggested that the flux of unusual fatty acids through phosphatidylcholine (PC) represents a major bottleneck for high accumulation of such fatty acids in triacylglycerol (TAG). We show here that a phospholipase C-like protein (RcPLCL1) from castor bean, which accumulates nearly 90% of the hydroxylated ricinoleic acid in its seed TAG, increases the amount of hydroxy fatty acids (HFAs) when co-expresses with the fatty acid hydroxylase (RcFAH12) in transgenic seed of Camelina sativa. RcPLCL1 shows hydrolyzing activities on both PC and phosphatidylinositol substrates in our in vitro assay conditions. The PC-PLC activity of the RcPLCL1 may have increased the efficiency of HFA-PC to diacylglycerol conversion, which explains our observation of increased HFA contents in TAG concomitant with decreased HFA in the membrane lipid PC during seed development. Consequently, this may also alleviate the potential detrimental effect of HFA on germination of the engineered camelina seeds. Our results provide new knowledge that will help design effective strategies to engineer high levels of HFAs in transgenic oilseeds.

Authors+Show Affiliations

Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, United States.Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30443260

Citation

Aryal, Niranjan, and Chaofu Lu. "A Phospholipase C-Like Protein From Ricinus Communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina Sativa." Frontiers in Plant Science, vol. 9, 2018, p. 1576.
Aryal N, Lu C. A Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativa. Front Plant Sci. 2018;9:1576.
Aryal, N., & Lu, C. (2018). A Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativa. Frontiers in Plant Science, 9, 1576. https://doi.org/10.3389/fpls.2018.01576
Aryal N, Lu C. A Phospholipase C-Like Protein From Ricinus Communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina Sativa. Front Plant Sci. 2018;9:1576. PubMed PMID: 30443260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativa. AU - Aryal,Niranjan, AU - Lu,Chaofu, Y1 - 2018/11/01/ PY - 2018/04/10/received PY - 2018/10/09/accepted PY - 2018/11/17/entrez PY - 2018/11/18/pubmed PY - 2018/11/18/medline KW - Camelina sativa KW - castor KW - hydroxy fatty acids KW - phospholipase C-like KW - triacylglycerol SP - 1576 EP - 1576 JF - Frontiers in plant science JO - Front Plant Sci VL - 9 N2 - There have been strong interests in producing unusual fatty acids in oilseed crops to provide renewable industrial feedstock. Results are so far largely disappointing since much lower amounts of such fatty acids accumulate in genetically engineered seeds than in their original natural sources. It has been suggested that the flux of unusual fatty acids through phosphatidylcholine (PC) represents a major bottleneck for high accumulation of such fatty acids in triacylglycerol (TAG). We show here that a phospholipase C-like protein (RcPLCL1) from castor bean, which accumulates nearly 90% of the hydroxylated ricinoleic acid in its seed TAG, increases the amount of hydroxy fatty acids (HFAs) when co-expresses with the fatty acid hydroxylase (RcFAH12) in transgenic seed of Camelina sativa. RcPLCL1 shows hydrolyzing activities on both PC and phosphatidylinositol substrates in our in vitro assay conditions. The PC-PLC activity of the RcPLCL1 may have increased the efficiency of HFA-PC to diacylglycerol conversion, which explains our observation of increased HFA contents in TAG concomitant with decreased HFA in the membrane lipid PC during seed development. Consequently, this may also alleviate the potential detrimental effect of HFA on germination of the engineered camelina seeds. Our results provide new knowledge that will help design effective strategies to engineer high levels of HFAs in transgenic oilseeds. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/30443260/A_Phospholipase_C_Like_Protein_From_Ricinus_communis_Increases_Hydroxy_Fatty_Acids_Accumulation_in_Transgenic_Seeds_of_Camelina_sativa_ DB - PRIME DP - Unbound Medicine ER -
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