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Identification of O-acetylserine(thiol)lyase (OASTL) genes in sorghum (Sorghum bicolor) and gene expression analysis under cadmium stress.
Mol Biol Rep 2019; 46(1):343-354MB

Abstract

Cysteine (Cys) is the first identified molecule in plant metabolism which includes both sulfur and nitrogen. It can be synthesized in three cellular compartments, containing chloroplast, cytoplasm and mitochondrion. The final step of cysteine biosynthesis is catalyzed by the O-acetylserine(thiol)lyase enzyme (OASTL, E.C. 4.2.99). In the present study, seven members of the OASTL gene family in the sorghum (Sorghum bicolor) genome were identified at a genome-wide scale and comparative bioinformatics analyses were performed between sorghum and Arabidopsis OASTLs. In all OASTL proteins, a pyridoxal-phosphate dependent domain structure (PALP, PF00291) was identified. The gene ontology annotations also revealed that all sorghum OASTL genes have KOG1252 (Cystathionine beta-synthase and related enzyme) and K01738 (cysteine synthase A) activities. In promotor sequences of OASTL genes, diverse cis-acting elements were found, including hormone and light responsiveness, abiotic stress responsiveness, and tissue-specific ones (meristem and endosperm). Sorghum OASTL genes demonstrated medium or high level expressions in anatomical parts and developmental stages based on the digital expression data. Expression of OASTL genes were also analyzed under cadmium (Cd) stress in sorghum by Real Time-quantitative PCR (RT-qPCR). The results exclusively showed that OASTL A1-2 gene was 1.12 fold up-regulated in roots, whereas cysteine synthase 26 was 2.25 fold down-regulated in leaves. The predicted 3D structure of OASTLs indicated some structural diversities as well as variations in the secondary structures.

Authors+Show Affiliations

Department of Agricultural Biotechnology, Akdeniz University, Antalya, Turkey. akbudak@akdeniz.edu.tr.Department of Crop and Animal Production, Duzce University, Cilimli Vocational School, Cilimli, Duzce, Turkey. ertugrulfiliz@gmail.com.Department of Agricultural Biotechnology, Akdeniz University, Antalya, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30443823

Citation

Akbudak, M Aydın, et al. "Identification of O-acetylserine(thiol)lyase (OASTL) Genes in Sorghum (Sorghum Bicolor) and Gene Expression Analysis Under Cadmium Stress." Molecular Biology Reports, vol. 46, no. 1, 2019, pp. 343-354.
Akbudak MA, Filiz E, Uylas S. Identification of O-acetylserine(thiol)lyase (OASTL) genes in sorghum (Sorghum bicolor) and gene expression analysis under cadmium stress. Mol Biol Rep. 2019;46(1):343-354.
Akbudak, M. A., Filiz, E., & Uylas, S. (2019). Identification of O-acetylserine(thiol)lyase (OASTL) genes in sorghum (Sorghum bicolor) and gene expression analysis under cadmium stress. Molecular Biology Reports, 46(1), pp. 343-354. doi:10.1007/s11033-018-4477-0.
Akbudak MA, Filiz E, Uylas S. Identification of O-acetylserine(thiol)lyase (OASTL) Genes in Sorghum (Sorghum Bicolor) and Gene Expression Analysis Under Cadmium Stress. Mol Biol Rep. 2019;46(1):343-354. PubMed PMID: 30443823.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of O-acetylserine(thiol)lyase (OASTL) genes in sorghum (Sorghum bicolor) and gene expression analysis under cadmium stress. AU - Akbudak,M Aydın, AU - Filiz,Ertugrul, AU - Uylas,Senem, Y1 - 2018/11/15/ PY - 2018/07/26/received PY - 2018/11/07/accepted PY - 2018/11/18/pubmed PY - 2019/7/2/medline PY - 2018/11/17/entrez KW - Bioinformatics KW - Cadmium KW - Co-expression KW - Cysteine KW - Sorghum KW - Sulfur SP - 343 EP - 354 JF - Molecular biology reports JO - Mol. Biol. Rep. VL - 46 IS - 1 N2 - Cysteine (Cys) is the first identified molecule in plant metabolism which includes both sulfur and nitrogen. It can be synthesized in three cellular compartments, containing chloroplast, cytoplasm and mitochondrion. The final step of cysteine biosynthesis is catalyzed by the O-acetylserine(thiol)lyase enzyme (OASTL, E.C. 4.2.99). In the present study, seven members of the OASTL gene family in the sorghum (Sorghum bicolor) genome were identified at a genome-wide scale and comparative bioinformatics analyses were performed between sorghum and Arabidopsis OASTLs. In all OASTL proteins, a pyridoxal-phosphate dependent domain structure (PALP, PF00291) was identified. The gene ontology annotations also revealed that all sorghum OASTL genes have KOG1252 (Cystathionine beta-synthase and related enzyme) and K01738 (cysteine synthase A) activities. In promotor sequences of OASTL genes, diverse cis-acting elements were found, including hormone and light responsiveness, abiotic stress responsiveness, and tissue-specific ones (meristem and endosperm). Sorghum OASTL genes demonstrated medium or high level expressions in anatomical parts and developmental stages based on the digital expression data. Expression of OASTL genes were also analyzed under cadmium (Cd) stress in sorghum by Real Time-quantitative PCR (RT-qPCR). The results exclusively showed that OASTL A1-2 gene was 1.12 fold up-regulated in roots, whereas cysteine synthase 26 was 2.25 fold down-regulated in leaves. The predicted 3D structure of OASTLs indicated some structural diversities as well as variations in the secondary structures. SN - 1573-4978 UR - https://www.unboundmedicine.com/medline/citation/30443823/Identification_of_O_acetylserine_thiol_lyase__OASTL__genes_in_sorghum__Sorghum_bicolor__and_gene_expression_analysis_under_cadmium_stress_ L2 - https://doi.org/10.1007/s11033-018-4477-0 DB - PRIME DP - Unbound Medicine ER -