Ultrasensitive Photoelectrochemical Assay with PTB7-Th/CdTe Quantum Dots Sensitized Structure as Signal Tag and Benzo-4-chlorohexadienone Precipitate as Efficient Quencher.
Anal Chem. 2018 12 18; 90(24):14521-14526.AC

Abstract

Herein, an ultrasensitive photoelectrochemical (PEC) assay was developed for the monitoring of microRNA-141 (miRNA-141) based on poly{4,8-bis[5-(2-ethylhexyl)thiophen-2-yl]benzo[1,2- b:4,5- b']dithiophene-2,6-diyl- alt-3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4- b]thiophene-4,6-diyl}/CdTe quantum dots (PTB7-Th/CdTe QDs) sensitized structure as signal tag and the benzo-4-chlorohexadienone (4-CD) precipitate as efficient quencher. PTB7-Th and CdTe QDs were successively modified on the electrode surface to form a novel sensitized structure with eminent photovoltaic performances and surpassing film-forming ability. The PTB7-Th/CdTe QDs sensitized structure served as signal tag for achieving a strong initial PEC signal. Besides, a tiny amount of target (miRNA-141) could be transformed into numerous DNA products via enzyme-assisted target cycling procedure, which further triggered the formation of a DNA supersandwich structure on the electrode surface for loading abundant manganese porphyrin (MnPP). Thereafter, MnPP as mimetic enzyme could catalyze 4-chloro-1-naphthol (4-CN) to generate 4-CD precipitate on the sensing interface in the presence of H2O2, which could efficiently block electron transfer, leading to a significantly quenched PEC signal for determination of miRNA-141. The designed PEC biosensor performed a wide detection range from 0.1 fM to 1 nM with a low detection limit of 33 aM for miRNA-141, which paved a new avenue for highly accurate and ultrasensitive monitoring of multifarious analytes in bioanalysis and clinical diagnosis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li M

Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering , Southwest University , Chongqing 400715 , People's Republic of China.

MeSH

Biosensing TechniquesCadmium CompoundsCatalysisElectrochemical TechniquesHumansHydrogen PeroxideLimit of DetectionMicroRNAsNaphtholsQuantum DotsTelluriumThiophenes

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30444350

Citation

Li, Mengjie, et al. "Ultrasensitive Photoelectrochemical Assay With PTB7-Th/CdTe Quantum Dots Sensitized Structure as Signal Tag and Benzo-4-chlorohexadienone Precipitate as Efficient Quencher." Analytical Chemistry, vol. 90, no. 24, 2018, pp. 14521-14526.

Li M, Tian X, Liang W, et al. Ultrasensitive Photoelectrochemical Assay with PTB7-Th/CdTe Quantum Dots Sensitized Structure as Signal Tag and Benzo-4-chlorohexadienone Precipitate as Efficient Quencher. Anal Chem. 2018;90(24):14521-14526.

Li, M., Tian, X., Liang, W., Yuan, R., & Chai, Y. (2018). Ultrasensitive Photoelectrochemical Assay with PTB7-Th/CdTe Quantum Dots Sensitized Structure as Signal Tag and Benzo-4-chlorohexadienone Precipitate as Efficient Quencher. Analytical Chemistry, 90(24), 14521-14526. https://doi.org/10.1021/acs.analchem.8b04370

Li M, et al. Ultrasensitive Photoelectrochemical Assay With PTB7-Th/CdTe Quantum Dots Sensitized Structure as Signal Tag and Benzo-4-chlorohexadienone Precipitate as Efficient Quencher. Anal Chem. 2018 12 18;90(24):14521-14526. PubMed PMID: 30444350.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Ultrasensitive Photoelectrochemical Assay with PTB7-Th/CdTe Quantum Dots Sensitized Structure as Signal Tag and Benzo-4-chlorohexadienone Precipitate as Efficient Quencher. AU - Li,Mengjie, AU - Tian,Xin, AU - Liang,Wenbin, AU - Yuan,Ruo, AU - Chai,Yaqin, Y1 - 2018/11/29/ PY - 2018/11/18/pubmed PY - 2019/5/17/medline PY - 2018/11/17/entrez SP - 14521 EP - 14526 JF - Analytical chemistry JO - Anal Chem VL - 90 IS - 24 N2 - Herein, an ultrasensitive photoelectrochemical (PEC) assay was developed for the monitoring of microRNA-141 (miRNA-141) based on poly{4,8-bis[5-(2-ethylhexyl)thiophen-2-yl]benzo[1,2- b:4,5- b']dithiophene-2,6-diyl- alt-3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4- b]thiophene-4,6-diyl}/CdTe quantum dots (PTB7-Th/CdTe QDs) sensitized structure as signal tag and the benzo-4-chlorohexadienone (4-CD) precipitate as efficient quencher. PTB7-Th and CdTe QDs were successively modified on the electrode surface to form a novel sensitized structure with eminent photovoltaic performances and surpassing film-forming ability. The PTB7-Th/CdTe QDs sensitized structure served as signal tag for achieving a strong initial PEC signal. Besides, a tiny amount of target (miRNA-141) could be transformed into numerous DNA products via enzyme-assisted target cycling procedure, which further triggered the formation of a DNA supersandwich structure on the electrode surface for loading abundant manganese porphyrin (MnPP). Thereafter, MnPP as mimetic enzyme could catalyze 4-chloro-1-naphthol (4-CN) to generate 4-CD precipitate on the sensing interface in the presence of H2O2, which could efficiently block electron transfer, leading to a significantly quenched PEC signal for determination of miRNA-141. The designed PEC biosensor performed a wide detection range from 0.1 fM to 1 nM with a low detection limit of 33 aM for miRNA-141, which paved a new avenue for highly accurate and ultrasensitive monitoring of multifarious analytes in bioanalysis and clinical diagnosis. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/30444350/Ultrasensitive_Photoelectrochemical_Assay_with_PTB7_Th/CdTe_Quantum_Dots_Sensitized_Structure_as_Signal_Tag_and_Benzo_4_chlorohexadienone_Precipitate_as_Efficient_Quencher_ DB - PRIME DP - Unbound Medicine ER -

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Ultrasensitive Photoelectrochemical Assay with PTB7-Th/CdTe Quantum Dots Sensitized Structure as Signal Tag and Benzo-4-chlorohexadienone Precipitate as Efficient Quencher.Anal Chem. 2018 12 18; 90(24):14521-14526.AC