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Frequency of DNA gyrase and topoisomerase IV mutations and plasmid-mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in Azerbaijan, Iran.
J Glob Antimicrob Resist. 2019 06; 17:39-43.JG

Abstract

OBJECTIVES

This study assessed genetic alterations in gyrA, gyrB, parC and parE and the prevalence of plasmid-mediated quinolone resistance (PMQR) genes among Escherichia coli and Klebsiella pneumoniae isolates from urinary tract infections (UTIs) in Azerbaijan, Iran.

METHODS

A total of 205 clinical isolates of E. coli (n=177) and K. pneumoniae (n=28) were obtained from UTIs. Antimicrobial susceptibility was determined by disk diffusion and agar dilution assays. The presence of PMQR genes was determined by PCR, and sequencing of the gyrA, gyrB, parC and parE was performed.

RESULTS

The rate of fluoroquinolone (FQ) resistance among the isolates was 77.1%. The Ser83Leu mutation in gyrA was observed in all 60 FQ-resistant isolates selected for direct sequencing. The second most common mutation in gyrA was Asp87Asn. Frequent mutations in parC were Ser80Ile and Glu84Val. Ser359Ala+Ser367Thr and Gly385Cys mutations in gyrB were identified in one isolate each of K. pneumoniae and E. coli, respectively. The parE gene had mutations at Ile529Leu, Ser458Ala and Leu416Phe. Overall, PMQR determinants were identified in 90% of E. coli and 100% of K. pneumoniae. The prevalence of PMQR genes was as follows: aac(6')-Ib-cr, 71.7%; oqxB, 51.7%; oqxA, 36.7%; qnrB, 28.3%; qnrS, 21.7%; qnrD, 16.7%; qepA, 5.0%; qnrA, 1.7%; and qnrC, 1.7%.

CONCLUSIONS

FQ resistance rates were high. Mutations in DNA gyrase and topoisomerase IV and the prevalence of PMQR genes in E. coli and K. pneumoniae isolates were alarming. Moreover, the combination of these resistance mechanisms plays an important role in high-level FQ resistance.

Authors+Show Affiliations

Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Microbiology Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Microbiology Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Microbiology Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Microbiology Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Faculty of Veterinary Medicine, Islamic Azad University, Urmia, Iran.Microbiology Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Microbiology Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: rzgottaslo@yahoo.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30445211

Citation

Azargun, Robab, et al. "Frequency of DNA Gyrase and Topoisomerase IV Mutations and Plasmid-mediated Quinolone Resistance Genes Among Escherichia Coli and Klebsiella Pneumoniae Isolated From Urinary Tract Infections in Azerbaijan, Iran." Journal of Global Antimicrobial Resistance, vol. 17, 2019, pp. 39-43.
Azargun R, Soroush Barhaghi MH, Samadi Kafil H, et al. Frequency of DNA gyrase and topoisomerase IV mutations and plasmid-mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in Azerbaijan, Iran. J Glob Antimicrob Resist. 2019;17:39-43.
Azargun, R., Soroush Barhaghi, M. H., Samadi Kafil, H., Ahangar Oskouee, M., Sadeghi, V., Memar, M. Y., & Ghotaslou, R. (2019). Frequency of DNA gyrase and topoisomerase IV mutations and plasmid-mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in Azerbaijan, Iran. Journal of Global Antimicrobial Resistance, 17, 39-43. https://doi.org/10.1016/j.jgar.2018.11.003
Azargun R, et al. Frequency of DNA Gyrase and Topoisomerase IV Mutations and Plasmid-mediated Quinolone Resistance Genes Among Escherichia Coli and Klebsiella Pneumoniae Isolated From Urinary Tract Infections in Azerbaijan, Iran. J Glob Antimicrob Resist. 2019;17:39-43. PubMed PMID: 30445211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequency of DNA gyrase and topoisomerase IV mutations and plasmid-mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in Azerbaijan, Iran. AU - Azargun,Robab, AU - Soroush Barhaghi,Mohammad Hossein, AU - Samadi Kafil,Hossein, AU - Ahangar Oskouee,Mahin, AU - Sadeghi,Vahid, AU - Memar,Mohammad Yousef, AU - Ghotaslou,Reza, Y1 - 2018/11/13/ PY - 2018/10/23/received PY - 2018/11/04/accepted PY - 2018/11/18/pubmed PY - 2020/5/20/medline PY - 2018/11/17/entrez KW - DNA gyrase KW - Enterobacteriaceae KW - PMQR KW - Plasmid-mediated quinolone resistance KW - Topoisomerase IV KW - Urinary tract infection SP - 39 EP - 43 JF - Journal of global antimicrobial resistance JO - J Glob Antimicrob Resist VL - 17 N2 - OBJECTIVES: This study assessed genetic alterations in gyrA, gyrB, parC and parE and the prevalence of plasmid-mediated quinolone resistance (PMQR) genes among Escherichia coli and Klebsiella pneumoniae isolates from urinary tract infections (UTIs) in Azerbaijan, Iran. METHODS: A total of 205 clinical isolates of E. coli (n=177) and K. pneumoniae (n=28) were obtained from UTIs. Antimicrobial susceptibility was determined by disk diffusion and agar dilution assays. The presence of PMQR genes was determined by PCR, and sequencing of the gyrA, gyrB, parC and parE was performed. RESULTS: The rate of fluoroquinolone (FQ) resistance among the isolates was 77.1%. The Ser83Leu mutation in gyrA was observed in all 60 FQ-resistant isolates selected for direct sequencing. The second most common mutation in gyrA was Asp87Asn. Frequent mutations in parC were Ser80Ile and Glu84Val. Ser359Ala+Ser367Thr and Gly385Cys mutations in gyrB were identified in one isolate each of K. pneumoniae and E. coli, respectively. The parE gene had mutations at Ile529Leu, Ser458Ala and Leu416Phe. Overall, PMQR determinants were identified in 90% of E. coli and 100% of K. pneumoniae. The prevalence of PMQR genes was as follows: aac(6')-Ib-cr, 71.7%; oqxB, 51.7%; oqxA, 36.7%; qnrB, 28.3%; qnrS, 21.7%; qnrD, 16.7%; qepA, 5.0%; qnrA, 1.7%; and qnrC, 1.7%. CONCLUSIONS: FQ resistance rates were high. Mutations in DNA gyrase and topoisomerase IV and the prevalence of PMQR genes in E. coli and K. pneumoniae isolates were alarming. Moreover, the combination of these resistance mechanisms plays an important role in high-level FQ resistance. SN - 2213-7173 UR - https://www.unboundmedicine.com/medline/citation/30445211/Frequency_of_DNA_gyrase_and_topoisomerase_IV_mutations_and_plasmid_mediated_quinolone_resistance_genes_among_Escherichia_coli_and_Klebsiella_pneumoniae_isolated_from_urinary_tract_infections_in_Azerbaijan_Iran_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2213-7165(18)30219-4 DB - PRIME DP - Unbound Medicine ER -