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Full genome characterization of two novel Alpha-coronavirus species from Italian bats.
Virus Res. 2019 01 15; 260:60-66.VR

Abstract

Coronaviruses (CoVs) have been detected worldwide in several bat species, which are considered the main reservoir. The attention to the high diversity of CoVs hosted by bats has increased during the last decade due to the high number of human infections caused by two zoonotic Beta-CoVs, SARS-CoV and MERS-CoV, that cause several respiratory diseases. Among coronaviruses, two Alpha-CoV strains (HuCoV-229E and HuCoV-NL63) cause mild respiratory disease that can change to severe disease in children, elderly and individuals affected by illnesses. Phylogenetic analysis conducted on bat Alpha-CoV strains revealed their evolutive correlation to human strains, suggesting their origin in bats. The genome of CoVs is characterized by a high frequency of mutations and recombination events, increasing their ability to switch hosts and their zoonotic potential. In this study, three strains of Alpha-CoV genera detected in Italian bats (Pipistrellus kuhlii) were fully sequenced by Next Generation Sequencing (NGS) and characterized. The complete genome analysis showed the correlation of the Italians strains with a Chinese strain detected in 2013 and, based on CoV molecular species demarcation, two new Alpha-CoV species were established. The analysis of a fragment of the RNA-dependent RNA polymerase (RdRp) showed the correlation of the Italian strains with CoVs that was only detected in the bat Pipistrellus genera (Pipistrellus kuhlii and Pipistrellus Pipistrellus) in European countries.

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Authors+Show Affiliations

De Sabato L
Department of Sciences, University Roma Tre, Viale Guglielmo Marconi 446, 00146, Rome, Italy; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
Lelli D
Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy.
Faccin F
Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy.
Canziani S
Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy.
Di Bartolo I
Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
Vaccari G
Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy. Electronic address: gabriele.vaccari@iss.it.
Moreno A
Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy.

MeSH

AnimalsChiropteraComputational BiologyCoronavirusCoronavirus InfectionsGenome, ViralHigh-Throughput Nucleotide SequencingItalyPhylogenySequence Homology

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30447246

Citation

De Sabato, Luca, et al. "Full Genome Characterization of Two Novel Alpha-coronavirus Species From Italian Bats." Virus Research, vol. 260, 2019, pp. 60-66.
De Sabato L, Lelli D, Faccin F, et al. Full genome characterization of two novel Alpha-coronavirus species from Italian bats. Virus Res. 2019;260:60-66.
De Sabato, L., Lelli, D., Faccin, F., Canziani, S., Di Bartolo, I., Vaccari, G., & Moreno, A. (2019). Full genome characterization of two novel Alpha-coronavirus species from Italian bats. Virus Research, 260, 60-66. https://doi.org/10.1016/j.virusres.2018.11.007
De Sabato L, et al. Full Genome Characterization of Two Novel Alpha-coronavirus Species From Italian Bats. Virus Res. 2019 01 15;260:60-66. PubMed PMID: 30447246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Full genome characterization of two novel Alpha-coronavirus species from Italian bats. AU - De Sabato,Luca, AU - Lelli,Davide, AU - Faccin,Francesca, AU - Canziani,Sabrina, AU - Di Bartolo,Ilaria, AU - Vaccari,Gabriele, AU - Moreno,Ana, Y1 - 2018/11/15/ PY - 2018/09/25/received PY - 2018/11/07/revised PY - 2018/11/13/accepted PY - 2018/11/18/pubmed PY - 2019/1/23/medline PY - 2018/11/18/entrez KW - Alpha-CoV viruses KW - Bats KW - Full genome sequencing KW - Italy SP - 60 EP - 66 JF - Virus research JO - Virus Res VL - 260 N2 - Coronaviruses (CoVs) have been detected worldwide in several bat species, which are considered the main reservoir. The attention to the high diversity of CoVs hosted by bats has increased during the last decade due to the high number of human infections caused by two zoonotic Beta-CoVs, SARS-CoV and MERS-CoV, that cause several respiratory diseases. Among coronaviruses, two Alpha-CoV strains (HuCoV-229E and HuCoV-NL63) cause mild respiratory disease that can change to severe disease in children, elderly and individuals affected by illnesses. Phylogenetic analysis conducted on bat Alpha-CoV strains revealed their evolutive correlation to human strains, suggesting their origin in bats. The genome of CoVs is characterized by a high frequency of mutations and recombination events, increasing their ability to switch hosts and their zoonotic potential. In this study, three strains of Alpha-CoV genera detected in Italian bats (Pipistrellus kuhlii) were fully sequenced by Next Generation Sequencing (NGS) and characterized. The complete genome analysis showed the correlation of the Italians strains with a Chinese strain detected in 2013 and, based on CoV molecular species demarcation, two new Alpha-CoV species were established. The analysis of a fragment of the RNA-dependent RNA polymerase (RdRp) showed the correlation of the Italian strains with CoVs that was only detected in the bat Pipistrellus genera (Pipistrellus kuhlii and Pipistrellus Pipistrellus) in European countries. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/30447246/Full_genome_characterization_of_two_novel_Alpha_coronavirus_species_from_Italian_bats_ DB - PRIME DP - Unbound Medicine ER -