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In Vitro Antioxidant and Anti-Propionibacterium acnes Activities of Cold Water, Hot Water, and Methanol Extracts, and Their Respective Ethyl Acetate Fractions, from Sanguisorba officinalis L. Roots.
Molecules. 2018 Nov 16; 23(11)M

Abstract

Identification of medicinal plants and naturally derived compounds as new natural antioxidant and antibacterial sources for topical acne treatment has long been important. To determine anti-Propionibacterium acnes activity and in vitro antioxidant activities, Sanguisorba officinalis L. root (SOR) was extracted with cold water (CWE), hot water (HWE), and methanol (ME), and each extract was fractionated successively with hexane, ethyl acetate (EA), and butanol to determine whether the activities could be attributed to the total phenolic, flavonoid, terpenoid, and condensed tannin contents. Pearson's correlation coefficients were analyzed between the respective variables. The SOR CWE, HWE, ME, and their respective EA fractions showed anti-P. acnes activity based on the paper disc diffusion method on agar plates, minimum inhibitory concentration (MIC), and minimal bactericidal concentration (MBC). The MIC against P. acnes had a moderate (+) correlation with the total phenolic content, but not with the other measures. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity (SC) had a strong (⁻) correlation with the total phenolic content and a moderate (⁻) correlation with the total flavonoid content. The total antioxidant capacity had a strong (+) correlation with the condensed tannin content. Linoleic acid peroxidation inhibition had a strong (⁻) correlation with the total phenolic content. To elucidate the major active phytochemicals in the CWE-EA, HWE-EA, and ME-EA fractions, high performance liquid chromatography-ultraviolet (HPLC-UV) and ultra high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) were performed. The HPLC-UV analysis showed the presence of nine compounds in common (arjunic acid and/or euscaphic acid, gallic acid, kaempferol, caffeic acid, ferulic acid, tannic acid, and coumarin, quercetin). The UHPLC-QTOF-MS analysis showed the presence of nine compounds in common (gallic acid; caffeic acid; umbelliferone; arjunic acid, euscaphic acid, and/or tormentic acid; pomolic acid; rosamultic acid; and benzoic acid). When standards of the identified phytochemicals were tested against the same bacterium, quercetin, coumarin, and euscaphic acid showed antibacterial activity against P. acnes.

Authors+Show Affiliations

Department of Biology & Medicinal Science, Pai Chai University, Daejeon 35345, Korea. khboy111@pcu.ac.kr.Department of Biology & Medicinal Science, Pai Chai University, Daejeon 35345, Korea. 5star@pcu.ac.kr.Department of Biology & Medicinal Science, Pai Chai University, Daejeon 35345, Korea. creator1018@pcu.ac.kr.Department of Biology & Medicinal Science, Pai Chai University, Daejeon 35345, Korea. hijsm91@gmail.com.Department of Biology & Medicinal Science, Pai Chai University, Daejeon 35345, Korea. thdgml6245@naver.com.Department of Biology & Medicinal Science, Pai Chai University, Daejeon 35345, Korea. jungmo9@gmail.com.Department of Biology & Medicinal Science, Pai Chai University, Daejeon 35345, Korea. choicw@pcu.ac.kr.Daeduck Lab. Co. Ltd., Daejeon 34024, Korea. hpjhon@daeducklab.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30453560

Citation

Kim, Seongdae, et al. "In Vitro Antioxidant and Anti-Propionibacterium Acnes Activities of Cold Water, Hot Water, and Methanol Extracts, and Their Respective Ethyl Acetate Fractions, From Sanguisorba Officinalis L. Roots." Molecules (Basel, Switzerland), vol. 23, no. 11, 2018.
Kim S, Oh S, Noh HB, et al. In Vitro Antioxidant and Anti-Propionibacterium acnes Activities of Cold Water, Hot Water, and Methanol Extracts, and Their Respective Ethyl Acetate Fractions, from Sanguisorba officinalis L. Roots. Molecules. 2018;23(11).
Kim, S., Oh, S., Noh, H. B., Ji, S., Lee, S. H., Koo, J. M., Choi, C. W., & Jhun, H. P. (2018). In Vitro Antioxidant and Anti-Propionibacterium acnes Activities of Cold Water, Hot Water, and Methanol Extracts, and Their Respective Ethyl Acetate Fractions, from Sanguisorba officinalis L. Roots. Molecules (Basel, Switzerland), 23(11). https://doi.org/10.3390/molecules23113001
Kim S, et al. In Vitro Antioxidant and Anti-Propionibacterium Acnes Activities of Cold Water, Hot Water, and Methanol Extracts, and Their Respective Ethyl Acetate Fractions, From Sanguisorba Officinalis L. Roots. Molecules. 2018 Nov 16;23(11) PubMed PMID: 30453560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In Vitro Antioxidant and Anti-Propionibacterium acnes Activities of Cold Water, Hot Water, and Methanol Extracts, and Their Respective Ethyl Acetate Fractions, from Sanguisorba officinalis L. Roots. AU - Kim,Seongdae, AU - Oh,Sung, AU - Noh,Han Byul, AU - Ji,Seongmi, AU - Lee,Song Hee, AU - Koo,Jung Mo, AU - Choi,Chang Won, AU - Jhun,Hyun Pyo, Y1 - 2018/11/16/ PY - 2018/11/05/received PY - 2018/11/13/revised PY - 2018/11/14/accepted PY - 2018/11/21/entrez PY - 2018/11/21/pubmed PY - 2019/2/26/medline KW - Sanguisorba officinalis L. root KW - anti-Propionibacterium acnes activity KW - cold water extract KW - ethyl acetate fraction KW - hot water extract KW - in vitro antioxidant activities KW - methanol extract JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 11 N2 - Identification of medicinal plants and naturally derived compounds as new natural antioxidant and antibacterial sources for topical acne treatment has long been important. To determine anti-Propionibacterium acnes activity and in vitro antioxidant activities, Sanguisorba officinalis L. root (SOR) was extracted with cold water (CWE), hot water (HWE), and methanol (ME), and each extract was fractionated successively with hexane, ethyl acetate (EA), and butanol to determine whether the activities could be attributed to the total phenolic, flavonoid, terpenoid, and condensed tannin contents. Pearson's correlation coefficients were analyzed between the respective variables. The SOR CWE, HWE, ME, and their respective EA fractions showed anti-P. acnes activity based on the paper disc diffusion method on agar plates, minimum inhibitory concentration (MIC), and minimal bactericidal concentration (MBC). The MIC against P. acnes had a moderate (+) correlation with the total phenolic content, but not with the other measures. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity (SC) had a strong (⁻) correlation with the total phenolic content and a moderate (⁻) correlation with the total flavonoid content. The total antioxidant capacity had a strong (+) correlation with the condensed tannin content. Linoleic acid peroxidation inhibition had a strong (⁻) correlation with the total phenolic content. To elucidate the major active phytochemicals in the CWE-EA, HWE-EA, and ME-EA fractions, high performance liquid chromatography-ultraviolet (HPLC-UV) and ultra high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) were performed. The HPLC-UV analysis showed the presence of nine compounds in common (arjunic acid and/or euscaphic acid, gallic acid, kaempferol, caffeic acid, ferulic acid, tannic acid, and coumarin, quercetin). The UHPLC-QTOF-MS analysis showed the presence of nine compounds in common (gallic acid; caffeic acid; umbelliferone; arjunic acid, euscaphic acid, and/or tormentic acid; pomolic acid; rosamultic acid; and benzoic acid). When standards of the identified phytochemicals were tested against the same bacterium, quercetin, coumarin, and euscaphic acid showed antibacterial activity against P. acnes. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30453560/In_Vitro_Antioxidant_and_Anti_Propionibacterium_acnes_Activities_of_Cold_Water_Hot_Water_and_Methanol_Extracts_and_Their_Respective_Ethyl_Acetate_Fractions_from_Sanguisorba_officinalis_L__Roots_ L2 - https://www.mdpi.com/resolver?pii=molecules23113001 DB - PRIME DP - Unbound Medicine ER -