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Effect of Pentacyclic Triterpenoids-Rich Callus Extract of Chaenomeles japonica (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts.
Molecules 2018; 23(11)M

Abstract

The effect of the well-characterized callus extract of Chaenomeles japonica on viability, morphology, and proliferation of normal human skin fibroblasts was investigated. The phytochemical analysis was performed using the ultra-high performance liquid chromatography method. The total phenolic, phenolic acid, and flavonoid contents were determined spectrophotometrically. The antioxidant activity was investigated using the DPPH (1,1-Diphenyl-1-picrylhydrazyl Radical Scavenging), FRAP (Ferric Reducing Antioxidant Power), and CUPRAC (CUPric Reducing Antioxidant Capacity) assays. The callus growth index during passages was high as well as the content of pentacyclic triterpenoids. The microscopic observations of the fibroblast viability, morphology and the evaluation of the proliferation ratio (xCELLigence system) proved that the influence of callus extract on the fibroblasts was dose-dependent. The evaluated level of fibroblasts proliferation rate after 72 h of incubation with callus extract at concentration 12.5 µg L-1 was the highest compared to all the analyzed ligands. Moreover, callus extract administrated for 72 h caused a significant increase in the proliferation rate in comparison with the control group (5.7 ± 0.1 vs. 4.4 ± 0.9; p < 0.01). The preliminary studies carried out may suggest that the callus extract rich in triterpenoids may be a potential source of cosmetic ingredients with a beneficial effect on human skin.

Authors+Show Affiliations

Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 61-861 Poznań, Poland. kikowska@ump.edu.pl.Department of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, Poland. mchmielewska@ump.edu.pl.Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 61-861 Poznań, Poland. agata_nahorska@wp.pl.Department of Pharmacognosy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. elastudzinska@ump.edu.pl.Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland. jzuchowski@iung.pulawy.pl.Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland. asf@iung.pulawy.pl.Department of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, Poland. mkotwic@ump.edu.pl.Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 61-861 Poznań, Poland. bthiem@ump.edu.pl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30453634

Citation

Kikowska, Małgorzata Anna, et al. "Effect of Pentacyclic Triterpenoids-Rich Callus Extract of Chaenomeles Japonica (Thunb.) Lindl. Ex Spach On Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts." Molecules (Basel, Switzerland), vol. 23, no. 11, 2018.
Kikowska MA, Chmielewska M, Włodarczyk A, et al. Effect of Pentacyclic Triterpenoids-Rich Callus Extract of Chaenomeles japonica (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts. Molecules. 2018;23(11).
Kikowska, M. A., Chmielewska, M., Włodarczyk, A., Studzińska-Sroka, E., Żuchowski, J., Stochmal, A., ... Thiem, B. (2018). Effect of Pentacyclic Triterpenoids-Rich Callus Extract of Chaenomeles japonica (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts. Molecules (Basel, Switzerland), 23(11), doi:10.3390/molecules23113009.
Kikowska MA, et al. Effect of Pentacyclic Triterpenoids-Rich Callus Extract of Chaenomeles Japonica (Thunb.) Lindl. Ex Spach On Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts. Molecules. 2018 Nov 17;23(11) PubMed PMID: 30453634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Pentacyclic Triterpenoids-Rich Callus Extract of Chaenomeles japonica (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts. AU - Kikowska,Małgorzata Anna, AU - Chmielewska,Małgorzata, AU - Włodarczyk,Agata, AU - Studzińska-Sroka,Elżbieta, AU - Żuchowski,Jerzy, AU - Stochmal,Anna, AU - Kotwicka,Małgorzata, AU - Thiem,Barbara, Y1 - 2018/11/17/ PY - 2018/09/24/received PY - 2018/10/31/revised PY - 2018/11/15/accepted PY - 2018/11/21/entrez PY - 2018/11/21/pubmed PY - 2019/1/24/medline KW - Chaenomeles japonica KW - antioxidant activity KW - in vitro cultures KW - pentacyclic triterpenes KW - polyphenolics KW - skin fibroblasts JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 11 N2 - The effect of the well-characterized callus extract of Chaenomeles japonica on viability, morphology, and proliferation of normal human skin fibroblasts was investigated. The phytochemical analysis was performed using the ultra-high performance liquid chromatography method. The total phenolic, phenolic acid, and flavonoid contents were determined spectrophotometrically. The antioxidant activity was investigated using the DPPH (1,1-Diphenyl-1-picrylhydrazyl Radical Scavenging), FRAP (Ferric Reducing Antioxidant Power), and CUPRAC (CUPric Reducing Antioxidant Capacity) assays. The callus growth index during passages was high as well as the content of pentacyclic triterpenoids. The microscopic observations of the fibroblast viability, morphology and the evaluation of the proliferation ratio (xCELLigence system) proved that the influence of callus extract on the fibroblasts was dose-dependent. The evaluated level of fibroblasts proliferation rate after 72 h of incubation with callus extract at concentration 12.5 µg L-1 was the highest compared to all the analyzed ligands. Moreover, callus extract administrated for 72 h caused a significant increase in the proliferation rate in comparison with the control group (5.7 ± 0.1 vs. 4.4 ± 0.9; p < 0.01). The preliminary studies carried out may suggest that the callus extract rich in triterpenoids may be a potential source of cosmetic ingredients with a beneficial effect on human skin. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30453634/Effect_of_Pentacyclic_Triterpenoids_Rich_Callus_Extract_of_Chaenomeles_japonica__Thunb___Lindl__ex_Spach_on_Viability_Morphology_and_Proliferation_of_Normal_Human_Skin_Fibroblasts_ L2 - http://www.mdpi.com/resolver?pii=molecules23113009 DB - PRIME DP - Unbound Medicine ER -