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Toxicity of ZnO nanoparticles (NPs) to THP-1 macrophages: interactions with saturated or unsaturated free fatty acids.
Toxicol Mech Methods. 2019 May; 29(4):291-299.TM

Abstract

In a biological microenvironment, free fatty acids (FFA) as ubiquitous biological molecules might interact with nanoparticles (NPs) and consequently change the toxicological responses. However, whether the chemical structures of FFA could influence their interactions with NPs remain unknown. This study investigated the interactions between ZnO NPs and saturated or unsaturated FFA (complexed to BSA), namely stearic acid (SA, C18:0), oleic acid (OA, C18:1), and α-linolenic acid (ALA, C18:3). It was shown that BSA, SA, OA, and ALA increased the atomic force microscope (AFM) heights as well the polydispersity index (PDI) of ZnO NPs. BSA modestly protected THP-1 macrophages from ZnO NP exposure, whereas OA and ALA led to relatively less cyto-protective effects of BSA. Moreover, only co-exposure to ZnO NPs and SA significantly promoted the release of interleukin-8. BSA, SA, OA, and ALA equally changed intracellular ROS and Zn ions associated with ZnO exposure, but co-exposure to ZnO NPs and OA/ALA particularly activated the expression of endoplasmic reticulum stress-apoptosis genes. In combination, these results showed that FFA could influence the colloidal aspects and toxicological signaling pathway of ZnO NPs, which is dependent on the number of unsaturated bonds of FFA.

Authors+Show Affiliations

a Institute of Bast Fiber Crops , Chinese Academy of Agricultural Sciences , Changsha , P.R. China. b Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education, Laboratory of Biochemistry, College of Chemistry , Xiangtan University , Xiangtan , P.R. China.a Institute of Bast Fiber Crops , Chinese Academy of Agricultural Sciences , Changsha , P.R. China. b Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education, Laboratory of Biochemistry, College of Chemistry , Xiangtan University , Xiangtan , P.R. China.c National Engineering Research Center for Solid Preparation Technology of Chinese Medicines, Jiangxi University of Traditional Chinese Medicine s , Jiangxi Nanchang , PR China.b Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education, Laboratory of Biochemistry, College of Chemistry , Xiangtan University , Xiangtan , P.R. China.b Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education, Laboratory of Biochemistry, College of Chemistry , Xiangtan University , Xiangtan , P.R. China.a Institute of Bast Fiber Crops , Chinese Academy of Agricultural Sciences , Changsha , P.R. China.a Institute of Bast Fiber Crops , Chinese Academy of Agricultural Sciences , Changsha , P.R. China. b Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education, Laboratory of Biochemistry, College of Chemistry , Xiangtan University , Xiangtan , P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30461332

Citation

Jiang, Mengdie, et al. "Toxicity of ZnO Nanoparticles (NPs) to THP-1 Macrophages: Interactions With Saturated or Unsaturated Free Fatty Acids." Toxicology Mechanisms and Methods, vol. 29, no. 4, 2019, pp. 291-299.
Jiang M, Wu B, Sun Y, et al. Toxicity of ZnO nanoparticles (NPs) to THP-1 macrophages: interactions with saturated or unsaturated free fatty acids. Toxicol Mech Methods. 2019;29(4):291-299.
Jiang, M., Wu, B., Sun, Y., Ding, Y., Xie, Y., Liu, L., & Cao, Y. (2019). Toxicity of ZnO nanoparticles (NPs) to THP-1 macrophages: interactions with saturated or unsaturated free fatty acids. Toxicology Mechanisms and Methods, 29(4), 291-299. https://doi.org/10.1080/15376516.2018.1550130
Jiang M, et al. Toxicity of ZnO Nanoparticles (NPs) to THP-1 Macrophages: Interactions With Saturated or Unsaturated Free Fatty Acids. Toxicol Mech Methods. 2019;29(4):291-299. PubMed PMID: 30461332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxicity of ZnO nanoparticles (NPs) to THP-1 macrophages: interactions with saturated or unsaturated free fatty acids. AU - Jiang,Mengdie, AU - Wu,Bihan, AU - Sun,Yongbing, AU - Ding,Yanhuai, AU - Xie,Yixi, AU - Liu,Liangliang, AU - Cao,Yi, Y1 - 2019/01/16/ PY - 2018/11/22/pubmed PY - 2019/5/9/medline PY - 2018/11/22/entrez KW - THP-1 macrophages KW - ZnO nanoparticles (NPs) KW - endoplasmic reticulum (ER) stress KW - free fatty acids (FFA) KW - inflammation SP - 291 EP - 299 JF - Toxicology mechanisms and methods JO - Toxicol Mech Methods VL - 29 IS - 4 N2 - In a biological microenvironment, free fatty acids (FFA) as ubiquitous biological molecules might interact with nanoparticles (NPs) and consequently change the toxicological responses. However, whether the chemical structures of FFA could influence their interactions with NPs remain unknown. This study investigated the interactions between ZnO NPs and saturated or unsaturated FFA (complexed to BSA), namely stearic acid (SA, C18:0), oleic acid (OA, C18:1), and α-linolenic acid (ALA, C18:3). It was shown that BSA, SA, OA, and ALA increased the atomic force microscope (AFM) heights as well the polydispersity index (PDI) of ZnO NPs. BSA modestly protected THP-1 macrophages from ZnO NP exposure, whereas OA and ALA led to relatively less cyto-protective effects of BSA. Moreover, only co-exposure to ZnO NPs and SA significantly promoted the release of interleukin-8. BSA, SA, OA, and ALA equally changed intracellular ROS and Zn ions associated with ZnO exposure, but co-exposure to ZnO NPs and OA/ALA particularly activated the expression of endoplasmic reticulum stress-apoptosis genes. In combination, these results showed that FFA could influence the colloidal aspects and toxicological signaling pathway of ZnO NPs, which is dependent on the number of unsaturated bonds of FFA. SN - 1537-6524 UR - https://www.unboundmedicine.com/medline/citation/30461332/Toxicity_of_ZnO_nanoparticles__NPs__to_THP_1_macrophages:_interactions_with_saturated_or_unsaturated_free_fatty_acids_ L2 - https://www.tandfonline.com/doi/full/10.1080/15376516.2018.1550130 DB - PRIME DP - Unbound Medicine ER -