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Determination of Fentanyl Analog Exposure Using Dried Blood Spots with LC-MS-MS.
J Anal Toxicol. 2019 May 01; 43(4):266-276.JA

Abstract

Fentanyl, and the numerous drugs derived from it, are contributing to the opioid overdose epidemic currently underway in the USA. To identify human exposure to these growing public health threats, an LC-MS-MS method for 5 μL dried blood spots (DBS) was developed. This method was developed to detect exposure to 3-methylfentanyl, alfentanil, α-methylfentanyl, carfentanil, fentanyl, lofentanil, sufentanil, norcarfentanil, norfentanyl, norlofentanil, norsufentanil, and using a separate LC-MS-MS injection, cyclopropylfentanyl, acrylfentanyl, 2-furanylfentanyl, isobutyrylfentanyl, ocfentanil and methoxyacetylfentanyl. Preparation of materials into groups of compounds was used to accommodate an ever increasing need to incorporate newly identified fentanyls. This protocol was validated within a linear range of 1.00-100 ng/mL, with precision ≤12% CV and accuracy ≥93%, as reported for the pooled blood QC samples, and limits of detection as low as 0.10 ng/mL. The use of DBS to assess fentanyl analog exposures can facilitate rapid sample collection, transport, and preparation for analysis that could enhance surveillance and response efforts in the ongoing opioid overdose epidemic.

Authors+Show Affiliations

Battelle Memorial Institute at the Centers for Disease Control and Prevention, Atlanta, GA USA.Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA USA.Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA USA.Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA USA.Oak Ridge Institute of Science and Education Fellow at the Centers for Disease Control and Prevention, Atlanta, GA, USA.Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA USA.Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA USA.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

30462229

Citation

Seymour, Craig, et al. "Determination of Fentanyl Analog Exposure Using Dried Blood Spots With LC-MS-MS." Journal of Analytical Toxicology, vol. 43, no. 4, 2019, pp. 266-276.
Seymour C, Shaner RL, Feyereisen MC, et al. Determination of Fentanyl Analog Exposure Using Dried Blood Spots with LC-MS-MS. J Anal Toxicol. 2019;43(4):266-276.
Seymour, C., Shaner, R. L., Feyereisen, M. C., Wharton, R. E., Kaplan, P., Hamelin, E. I., & Johnson, R. C. (2019). Determination of Fentanyl Analog Exposure Using Dried Blood Spots with LC-MS-MS. Journal of Analytical Toxicology, 43(4), 266-276. https://doi.org/10.1093/jat/bky096
Seymour C, et al. Determination of Fentanyl Analog Exposure Using Dried Blood Spots With LC-MS-MS. J Anal Toxicol. 2019 May 1;43(4):266-276. PubMed PMID: 30462229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of Fentanyl Analog Exposure Using Dried Blood Spots with LC-MS-MS. AU - Seymour,Craig, AU - Shaner,Rebecca L, AU - Feyereisen,Melanie C, AU - Wharton,Rebekah E, AU - Kaplan,Pearl, AU - Hamelin,Elizabeth I, AU - Johnson,Rudolph C, PY - 2018/08/01/received PY - 2018/10/17/revised PY - 2018/11/22/pubmed PY - 2019/10/8/medline PY - 2018/11/22/entrez SP - 266 EP - 276 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 43 IS - 4 N2 - Fentanyl, and the numerous drugs derived from it, are contributing to the opioid overdose epidemic currently underway in the USA. To identify human exposure to these growing public health threats, an LC-MS-MS method for 5 μL dried blood spots (DBS) was developed. This method was developed to detect exposure to 3-methylfentanyl, alfentanil, α-methylfentanyl, carfentanil, fentanyl, lofentanil, sufentanil, norcarfentanil, norfentanyl, norlofentanil, norsufentanil, and using a separate LC-MS-MS injection, cyclopropylfentanyl, acrylfentanyl, 2-furanylfentanyl, isobutyrylfentanyl, ocfentanil and methoxyacetylfentanyl. Preparation of materials into groups of compounds was used to accommodate an ever increasing need to incorporate newly identified fentanyls. This protocol was validated within a linear range of 1.00-100 ng/mL, with precision ≤12% CV and accuracy ≥93%, as reported for the pooled blood QC samples, and limits of detection as low as 0.10 ng/mL. The use of DBS to assess fentanyl analog exposures can facilitate rapid sample collection, transport, and preparation for analysis that could enhance surveillance and response efforts in the ongoing opioid overdose epidemic. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/30462229/Determination_of_Fentanyl_Analog_Exposure_Using_Dried_Blood_Spots_with_LC_MS_MS_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bky096 DB - PRIME DP - Unbound Medicine ER -