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Plant RNases T2, but not Dicer-like proteins, are major players of tRNA-derived fragments biogenesis.
Nucleic Acids Res. 2019 01 25; 47(2):941-952.NA

Abstract

RNA fragments deriving from tRNAs (tRFs) exist in all branches of life and the repertoire of their biological functions regularly increases. Paradoxically, their biogenesis remains unclear. The human RNase A, Angiogenin, and the yeast RNase T2, Rny1p, generate long tRFs after cleavage in the anticodon region. The production of short tRFs after cleavage in the D or T regions is still enigmatic. Here, we show that the Arabidopsis Dicer-like proteins, DCL1-4, do not play a major role in the production of tRFs. Rather, we demonstrate that the Arabidopsis RNases T2, called RNS, are key players of both long and short tRFs biogenesis. Arabidopsis RNS show specific expression profiles. In particular, RNS1 and RNS3 are mainly found in the outer tissues of senescing seeds where they are the main endoribonucleases responsible of tRNA cleavage activity for tRFs production. In plants grown under phosphate starvation conditions, the induction of RNS1 is correlated with the accumulation of specific tRFs. Beyond plants, we also provide evidence that short tRFs can be produced by the yeast Rny1p and that, in vitro, human RNase T2 is also able to generate long and short tRFs. Our data suggest an evolutionary conserved feature of these enzymes in eukaryotes.

Authors+Show Affiliations

Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.Institut de biologie moléculaire des plantes-CNRS, Université de Strasbourg, 12 rue du Général Zimmer, F-67084 Strasbourg, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30462257

Citation

Megel, Cyrille, et al. "Plant RNases T2, but Not Dicer-like Proteins, Are Major Players of tRNA-derived Fragments Biogenesis." Nucleic Acids Research, vol. 47, no. 2, 2019, pp. 941-952.
Megel C, Hummel G, Lalande S, et al. Plant RNases T2, but not Dicer-like proteins, are major players of tRNA-derived fragments biogenesis. Nucleic Acids Res. 2019;47(2):941-952.
Megel, C., Hummel, G., Lalande, S., Ubrig, E., Cognat, V., Morelle, G., Salinas-Giegé, T., Duchêne, A. M., & Maréchal-Drouard, L. (2019). Plant RNases T2, but not Dicer-like proteins, are major players of tRNA-derived fragments biogenesis. Nucleic Acids Research, 47(2), 941-952. https://doi.org/10.1093/nar/gky1156
Megel C, et al. Plant RNases T2, but Not Dicer-like Proteins, Are Major Players of tRNA-derived Fragments Biogenesis. Nucleic Acids Res. 2019 01 25;47(2):941-952. PubMed PMID: 30462257.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plant RNases T2, but not Dicer-like proteins, are major players of tRNA-derived fragments biogenesis. AU - Megel,Cyrille, AU - Hummel,Guillaume, AU - Lalande,Stéphanie, AU - Ubrig,Elodie, AU - Cognat,Valérie, AU - Morelle,Geoffrey, AU - Salinas-Giegé,Thalia, AU - Duchêne,Anne-Marie, AU - Maréchal-Drouard,Laurence, PY - 2018/06/04/received PY - 2018/10/29/accepted PY - 2018/11/22/pubmed PY - 2019/8/21/medline PY - 2018/11/22/entrez SP - 941 EP - 952 JF - Nucleic acids research JO - Nucleic Acids Res VL - 47 IS - 2 N2 - RNA fragments deriving from tRNAs (tRFs) exist in all branches of life and the repertoire of their biological functions regularly increases. Paradoxically, their biogenesis remains unclear. The human RNase A, Angiogenin, and the yeast RNase T2, Rny1p, generate long tRFs after cleavage in the anticodon region. The production of short tRFs after cleavage in the D or T regions is still enigmatic. Here, we show that the Arabidopsis Dicer-like proteins, DCL1-4, do not play a major role in the production of tRFs. Rather, we demonstrate that the Arabidopsis RNases T2, called RNS, are key players of both long and short tRFs biogenesis. Arabidopsis RNS show specific expression profiles. In particular, RNS1 and RNS3 are mainly found in the outer tissues of senescing seeds where they are the main endoribonucleases responsible of tRNA cleavage activity for tRFs production. In plants grown under phosphate starvation conditions, the induction of RNS1 is correlated with the accumulation of specific tRFs. Beyond plants, we also provide evidence that short tRFs can be produced by the yeast Rny1p and that, in vitro, human RNase T2 is also able to generate long and short tRFs. Our data suggest an evolutionary conserved feature of these enzymes in eukaryotes. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/30462257/Plant_RNases_T2_but_not_Dicer_like_proteins_are_major_players_of_tRNA_derived_fragments_biogenesis_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gky1156 DB - PRIME DP - Unbound Medicine ER -