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Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle.
Int J Biol Macromol. 2019 Feb 15; 123:1221-1228.IJ

Abstract

In this study, novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP), by precipitation with cetylpyridinium, were characterized and their antioxidant and antibacterial activities were investigated. CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%). Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP. In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition. Sulfated polysaccharides were found to display important antibacterial activity against several Gram+ and Gram- bacteria. In addition, they exhibited strong antioxidant activities as showed by various in vitro tests. Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl. The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities. The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents.

Authors+Show Affiliations

Laboratory of Enzymatic Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, B.P. 1173-3038, Sfax, Tunisia; Higher Institute of Biotechnology of Beja, University of Jendouba, B.P. 382-9000, Beja, Tunisia. Electronic address: jridimourad@gmail.com.Laboratory of Enzymatic Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, B.P. 1173-3038, Sfax, Tunisia.Laboratory of Enzymatic Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, B.P. 1173-3038, Sfax, Tunisia.Laboratory of Enzymatic Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, B.P. 1173-3038, Sfax, Tunisia.Laboratory of Enzymatic Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, B.P. 1173-3038, Sfax, Tunisia.Laboratory of Enzymatic Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, B.P. 1173-3038, Sfax, Tunisia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30465838

Citation

Jridi, Mourad, et al. "Characterization and Assessment of Antioxidant and Antibacterial Activities of Sulfated Polysaccharides Extracted From Cuttlefish Skin and Muscle." International Journal of Biological Macromolecules, vol. 123, 2019, pp. 1221-1228.
Jridi M, Nasri R, Marzougui Z, et al. Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle. Int J Biol Macromol. 2019;123:1221-1228.
Jridi, M., Nasri, R., Marzougui, Z., Abdelhedi, O., Hamdi, M., & Nasri, M. (2019). Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle. International Journal of Biological Macromolecules, 123, 1221-1228. https://doi.org/10.1016/j.ijbiomac.2018.11.170
Jridi M, et al. Characterization and Assessment of Antioxidant and Antibacterial Activities of Sulfated Polysaccharides Extracted From Cuttlefish Skin and Muscle. Int J Biol Macromol. 2019 Feb 15;123:1221-1228. PubMed PMID: 30465838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle. AU - Jridi,Mourad, AU - Nasri,Rim, AU - Marzougui,Zeineb, AU - Abdelhedi,Ola, AU - Hamdi,Marwa, AU - Nasri,Moncef, Y1 - 2018/11/19/ PY - 2018/09/03/received PY - 2018/10/18/revised PY - 2018/11/17/accepted PY - 2018/11/23/pubmed PY - 2019/4/23/medline PY - 2018/11/23/entrez KW - Antioxidant and antibacterial activities KW - Cuttlefish sulfated polysaccharides KW - Infrared spectroscopic analysis SP - 1221 EP - 1228 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 123 N2 - In this study, novel polysaccharides extracted from cuttlefish skin (CSP) and muscle (CMP), by precipitation with cetylpyridinium, were characterized and their antioxidant and antibacterial activities were investigated. CMP showed the highest amounts of sulfated groups (6.6%), uronic acids (9.2%) and proteins (3.7%). Infrared spectroscopic analysis indicated the presence of sulfonyl (OSO) and acetyl (CH3CO-) groups for both CSP and CMP. In addition, CSP showed the presence of glucuronic acid (GlcA) and galacturonic acid (GalA) as major components, while CMP showed highest amount of GalA in its monosaccharide composition. Sulfated polysaccharides were found to display important antibacterial activity against several Gram+ and Gram- bacteria. In addition, they exhibited strong antioxidant activities as showed by various in vitro tests. Fractionation of cuttlefish polysaccharides, by DEAE-cellulose column showed one peak during the buffer elution phase and three major fractions for CMP and two peaks for CSP during the linear gradient of NaCl. The last eluting sulfated fraction from each sample, characterized by the highest negative charge, was found to exhibit the best antioxidant and antibacterial activities. The obtained results demonstrated that cuttlefish polysaccharides and their fractions could serve as natural antioxidant and antibacterial agents. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/30465838/Characterization_and_assessment_of_antioxidant_and_antibacterial_activities_of_sulfated_polysaccharides_extracted_from_cuttlefish_skin_and_muscle_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)34441-6 DB - PRIME DP - Unbound Medicine ER -