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LncRNA-TCL6 promotes early abortion and inhibits placenta implantation via the EGFR pathway.
Eur Rev Med Pharmacol Sci 2018; 22(21):7105-7112ER

Abstract

OBJECTIVE

The aim of this study was to investigate the role of long non-coding RNA (lncRNA) TCL6 in early abortion and to explore its underlying mechanism.

PATIENTS AND METHODS

The expression levels of lncRNA-TCL6 and epidermal growth factor receptor (EGFR) in placental tissues of normal pregnancy, threatened abortion pregnancy, spontaneous abortion pregnancy, and induced abortion pregnancy were detected by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), respectively. Trophoblast cells were transfected with siRNA to knock-down lncRNA-TCL6. Cell viability of trophoblast cells was detected by cell counting kit-8 (CCK-8) assay. The protein expression levels of EGFR, extracellular regulated protein kinases (ERK) and protein kinase B (AKT) in trophoblast cells after lncRNA-TCL6 knockdown were detected by Western blot. Rescue experiments were performed to investigate the relationship between EGFR and lncRNA-TCL6.

RESULTS

The expression of lncRNA-TCL6 in placenta tissues of threatened abortion pregnancy was significantly higher than that of normal pregnancy. Meanwhile, the expression of lncRNA-TCL6 in placenta tissues of spontaneous abortion pregnancy was also markedly higher than induced abortion pregnancy. However, the expression of EGFR showed an opposite trend. After knockdown of lncRNA-TCL6 in trophoblast cells, the protein expression levels of EGFR, ERK, and AKT were significantly increased when compared with those of the control group. CCK-8 assay indicated that cell viability was remarkably increased after knockdown of lncRNA-TCL6, which could be reversed by EGFR knockdown.

CONCLUSIONS

Compared with normal pregnancy, lncRNA-TCL6 was highly expressed in placental tissues of threatened abortion pregnancy. Moreover, the expression of lncRNA-TCL6 in placenta tissues of spontaneous abortion pregnancy was significantly higher than induced abortion pregnancy. Knockdown of lncRNA-TCL6 promoted the proliferation of trophoblast cells and inhibited the abortion via the EGFR signaling pathway.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Hanchuan People's Hospital of Hubei Province, Hanchuan, China. 454586966@qq.com.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30468451

Citation

Liu, L-P, and Y-B Gong. "LncRNA-TCL6 Promotes Early Abortion and Inhibits Placenta Implantation Via the EGFR Pathway." European Review for Medical and Pharmacological Sciences, vol. 22, no. 21, 2018, pp. 7105-7112.
Liu LP, Gong YB. LncRNA-TCL6 promotes early abortion and inhibits placenta implantation via the EGFR pathway. Eur Rev Med Pharmacol Sci. 2018;22(21):7105-7112.
Liu, L. P., & Gong, Y. B. (2018). LncRNA-TCL6 promotes early abortion and inhibits placenta implantation via the EGFR pathway. European Review for Medical and Pharmacological Sciences, 22(21), pp. 7105-7112. doi:10.26355/eurrev_201811_16242.
Liu LP, Gong YB. LncRNA-TCL6 Promotes Early Abortion and Inhibits Placenta Implantation Via the EGFR Pathway. Eur Rev Med Pharmacol Sci. 2018;22(21):7105-7112. PubMed PMID: 30468451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LncRNA-TCL6 promotes early abortion and inhibits placenta implantation via the EGFR pathway. AU - Liu,L-P, AU - Gong,Y-B, PY - 2018/11/24/entrez PY - 2018/11/24/pubmed PY - 2018/11/24/medline SP - 7105 EP - 7112 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 22 IS - 21 N2 - OBJECTIVE: The aim of this study was to investigate the role of long non-coding RNA (lncRNA) TCL6 in early abortion and to explore its underlying mechanism. PATIENTS AND METHODS: The expression levels of lncRNA-TCL6 and epidermal growth factor receptor (EGFR) in placental tissues of normal pregnancy, threatened abortion pregnancy, spontaneous abortion pregnancy, and induced abortion pregnancy were detected by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), respectively. Trophoblast cells were transfected with siRNA to knock-down lncRNA-TCL6. Cell viability of trophoblast cells was detected by cell counting kit-8 (CCK-8) assay. The protein expression levels of EGFR, extracellular regulated protein kinases (ERK) and protein kinase B (AKT) in trophoblast cells after lncRNA-TCL6 knockdown were detected by Western blot. Rescue experiments were performed to investigate the relationship between EGFR and lncRNA-TCL6. RESULTS: The expression of lncRNA-TCL6 in placenta tissues of threatened abortion pregnancy was significantly higher than that of normal pregnancy. Meanwhile, the expression of lncRNA-TCL6 in placenta tissues of spontaneous abortion pregnancy was also markedly higher than induced abortion pregnancy. However, the expression of EGFR showed an opposite trend. After knockdown of lncRNA-TCL6 in trophoblast cells, the protein expression levels of EGFR, ERK, and AKT were significantly increased when compared with those of the control group. CCK-8 assay indicated that cell viability was remarkably increased after knockdown of lncRNA-TCL6, which could be reversed by EGFR knockdown. CONCLUSIONS: Compared with normal pregnancy, lncRNA-TCL6 was highly expressed in placental tissues of threatened abortion pregnancy. Moreover, the expression of lncRNA-TCL6 in placenta tissues of spontaneous abortion pregnancy was significantly higher than induced abortion pregnancy. Knockdown of lncRNA-TCL6 promoted the proliferation of trophoblast cells and inhibited the abortion via the EGFR signaling pathway. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/30468451/LncRNA_TCL6_promotes_early_abortion_and_inhibits_placenta_implantation_via_the_EGFR_pathway_ L2 - https://www.europeanreview.org/article/16242 DB - PRIME DP - Unbound Medicine ER -
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