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In vivo and in vitro identity of site specific cleavages in the 5' non-coding region of ompA and bla mRNA in Escherichia coli.
EMBO J. 1988 Jul; 7(7):2269-75.EJ

Abstract

The bla and ompA gene transcripts were used as substrates to probe Escherichia coli extracts for ribonucleolytic activities. A site specific endoribonucleolytic activity was identified that cleaves ompA and bla mRNA. The cleavages occur in vitro and in vivo. For both the bla and ompA mRNA most of the cleavage sites which were identified map in the 5' non-coding region. The cleavages of the ompA transcript have been previously suggested to regulate the growth rate dependent stability of this mRNA. Thus we propose that the identified endoribonucleolytic activity may be involved in the degradation of mRNA. Analysis of mutants revealed that the cleavages are mediated by endonucleases which do not seem to be identical to RNase III, RNase E or RNase P.

Authors+Show Affiliations

Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3046939

Citation

Nilsson, G, et al. "In Vivo and in Vitro Identity of Site Specific Cleavages in the 5' Non-coding Region of ompA and Bla mRNA in Escherichia Coli." The EMBO Journal, vol. 7, no. 7, 1988, pp. 2269-75.
Nilsson G, Lundberg U, von Gabain A. In vivo and in vitro identity of site specific cleavages in the 5' non-coding region of ompA and bla mRNA in Escherichia coli. EMBO J. 1988;7(7):2269-75.
Nilsson, G., Lundberg, U., & von Gabain, A. (1988). In vivo and in vitro identity of site specific cleavages in the 5' non-coding region of ompA and bla mRNA in Escherichia coli. The EMBO Journal, 7(7), 2269-75.
Nilsson G, Lundberg U, von Gabain A. In Vivo and in Vitro Identity of Site Specific Cleavages in the 5' Non-coding Region of ompA and Bla mRNA in Escherichia Coli. EMBO J. 1988;7(7):2269-75. PubMed PMID: 3046939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo and in vitro identity of site specific cleavages in the 5' non-coding region of ompA and bla mRNA in Escherichia coli. AU - Nilsson,G, AU - Lundberg,U, AU - von Gabain,A, PY - 1988/7/1/pubmed PY - 1988/7/1/medline PY - 1988/7/1/entrez SP - 2269 EP - 75 JF - The EMBO journal JO - EMBO J VL - 7 IS - 7 N2 - The bla and ompA gene transcripts were used as substrates to probe Escherichia coli extracts for ribonucleolytic activities. A site specific endoribonucleolytic activity was identified that cleaves ompA and bla mRNA. The cleavages occur in vitro and in vivo. For both the bla and ompA mRNA most of the cleavage sites which were identified map in the 5' non-coding region. The cleavages of the ompA transcript have been previously suggested to regulate the growth rate dependent stability of this mRNA. Thus we propose that the identified endoribonucleolytic activity may be involved in the degradation of mRNA. Analysis of mutants revealed that the cleavages are mediated by endonucleases which do not seem to be identical to RNase III, RNase E or RNase P. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/3046939/In_vivo_and_in_vitro_identity_of_site_specific_cleavages_in_the_5'_non_coding_region_of_ompA_and_bla_mRNA_in_Escherichia_coli_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1988&volume=7&issue=7&spage=2269 DB - PRIME DP - Unbound Medicine ER -