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Effect of temperature on the microflora community composition in the digestive tract of the veined rapa whelk (Rapana venosa) revealed by 16S rRNA gene sequencing.

Abstract

The veined rapa whelk, Rapana venosa, a poikilotherm that is susceptible to temperature, is an important and valuable fishery resource in China but a major invader around the world. We studied the effects of abnormal temperature on the digestive tract microflora of R. venosa to investigate how temperature impacts its digestion and ingestion. We characterized the microflora in nine samples by sequencing the 16S rRNA gene. To assess the species diversity within the samples, effective tags were clustered at 97% similarity by default. Mycoplasma was the most abundant genus among the three groups, and the Proteobacteria phylum had the highest diversity. However, the microflora structure in the digestive tract was significantly different at different temperatures. The top five most abundant genera in the samples housed at 16 °C were Mycoplasma, Phyllobacterium, Aliivibrio, Psychromonas, and Delftia, whereas those in the samples housed under 22 °C were Mycoplasma, Phyllobacterium, Delftia, Spirochaeta_2, and Sphingomonas, and those in the samples housed at 28 °C were Mycoplasma, Phyllobacterium, Vibrio, Delftia, and Aliivibrio. The family Flavobacteriaceae was more abundant in R. venosa housed at 22 °C and 28 °C, whereas a significant decrease in Flavobacteriaceae abundance and a substantial increase in Mycoplasmataceae abundance were observed in R. venosa housed at 16 °C. The alteration in the digestive tract microflora might further affect the function of the R. venosa digestive tract. The results presented herein might provide further insight into investigations on the effects of temperature on the digestion and ingestion of gastropods.

Authors+Show Affiliations

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; University of Chinese Academy of Sciences, Beijing 100049, China.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; University of Chinese Academy of Sciences, Beijing 100049, China.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; University of Chinese Academy of Sciences, Beijing 100049, China.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.Qingdao Malvern College, Qingdao 266106, China.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: tzhang@qdio.ac.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30472607

Citation

Yang, Mei-Jie, et al. "Effect of Temperature On the Microflora Community Composition in the Digestive Tract of the Veined Rapa Whelk (Rapana Venosa) Revealed By 16S rRNA Gene Sequencing." Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, vol. 29, 2019, pp. 145-153.
Yang MJ, Song H, Sun LN, et al. Effect of temperature on the microflora community composition in the digestive tract of the veined rapa whelk (Rapana venosa) revealed by 16S rRNA gene sequencing. Comp Biochem Physiol Part D Genomics Proteomics. 2019;29:145-153.
Yang, M. J., Song, H., Sun, L. N., Yu, Z. L., Hu, Z., Wang, X. L., Zhu, J. Y., & Zhang, T. (2019). Effect of temperature on the microflora community composition in the digestive tract of the veined rapa whelk (Rapana venosa) revealed by 16S rRNA gene sequencing. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, 29, 145-153. https://doi.org/10.1016/j.cbd.2018.10.006
Yang MJ, et al. Effect of Temperature On the Microflora Community Composition in the Digestive Tract of the Veined Rapa Whelk (Rapana Venosa) Revealed By 16S rRNA Gene Sequencing. Comp Biochem Physiol Part D Genomics Proteomics. 2019;29:145-153. PubMed PMID: 30472607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of temperature on the microflora community composition in the digestive tract of the veined rapa whelk (Rapana venosa) revealed by 16S rRNA gene sequencing. AU - Yang,Mei-Jie, AU - Song,Hao, AU - Sun,Li-Na, AU - Yu,Zheng-Lin, AU - Hu,Zhi, AU - Wang,Xiao-Long, AU - Zhu,Jia-Yi, AU - Zhang,Tao, Y1 - 2018/10/23/ PY - 2018/10/18/received PY - 2018/10/21/accepted PY - 2018/11/26/pubmed PY - 2019/6/25/medline PY - 2018/11/26/entrez KW - Digestion and ingestion KW - Gastropod KW - Microflora KW - Rapana venosa KW - Temperature SP - 145 EP - 153 JF - Comparative biochemistry and physiology. Part D, Genomics & proteomics JO - Comp. Biochem. Physiol. Part D Genomics Proteomics VL - 29 N2 - The veined rapa whelk, Rapana venosa, a poikilotherm that is susceptible to temperature, is an important and valuable fishery resource in China but a major invader around the world. We studied the effects of abnormal temperature on the digestive tract microflora of R. venosa to investigate how temperature impacts its digestion and ingestion. We characterized the microflora in nine samples by sequencing the 16S rRNA gene. To assess the species diversity within the samples, effective tags were clustered at 97% similarity by default. Mycoplasma was the most abundant genus among the three groups, and the Proteobacteria phylum had the highest diversity. However, the microflora structure in the digestive tract was significantly different at different temperatures. The top five most abundant genera in the samples housed at 16 °C were Mycoplasma, Phyllobacterium, Aliivibrio, Psychromonas, and Delftia, whereas those in the samples housed under 22 °C were Mycoplasma, Phyllobacterium, Delftia, Spirochaeta_2, and Sphingomonas, and those in the samples housed at 28 °C were Mycoplasma, Phyllobacterium, Vibrio, Delftia, and Aliivibrio. The family Flavobacteriaceae was more abundant in R. venosa housed at 22 °C and 28 °C, whereas a significant decrease in Flavobacteriaceae abundance and a substantial increase in Mycoplasmataceae abundance were observed in R. venosa housed at 16 °C. The alteration in the digestive tract microflora might further affect the function of the R. venosa digestive tract. The results presented herein might provide further insight into investigations on the effects of temperature on the digestion and ingestion of gastropods. SN - 1878-0407 UR - https://www.unboundmedicine.com/medline/citation/30472607/Effect_of_temperature_on_the_microflora_community_composition_in_the_digestive_tract_of_the_veined_rapa_whelk_(Rapana_venosa)_revealed_by_16S_rRNA_gene_sequencing L2 - https://linkinghub.elsevier.com/retrieve/pii/S1744-117X(18)30119-9 DB - PRIME DP - Unbound Medicine ER -