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The investigation of the protective effects of nimesulide on experimental testicular ischemia-reperfusion injury in rats.
Rev Int Androl. 2020 Apr - Jun; 18(2):55-62.RI

Abstract

INTRODUCTION AND OBJECTIVES

Testicular ischemia/reperfusion (I/R) injury develops after torsion and following detorsion of the testis. Reactive oxygen species were produced and oxidative damage begins to occur due to I/R process. Nimesulide, which is a specific cyclooxygenase-2 inhibitor drug, have antioxidant, antiinflammatory, analgesics and antipyretic effects. We aimed to investigate biochemically and histopathologically effect of nimesulide on testis I/R injury in rats induced by the testicular torsion-detorsion.

MATERIAL AND METHODS

In this study, 24 albino Wistar male rats were divided into four groups (6 rats in each group): ischemia/reperfusion applied+50mg/kg nimesulide administrated (NIM-50), ischemia/reperfusion applied+100mg/kg nimesulide administrated (NIM-100), ischemia/reperfusion applied (IR) and Sham surgery (SS) groups. Nimesulide was administered to NIM-50 and NIM-100 groups at the 50mg/kg and 100mg/kg doses before 2h applied I/R procedures. The IR group were applied only I/R procedures, no drug treatment was applied. Animals were sacrificed under high dose anesthesia and left testes were extracted. Testes were examined biochemically and histopathologically.

RESULTS

Total glutathione (tGSH) and cyclooxygenase-1 (COX-1) levels were increased in the NIM-50 and NIM-100 groups compared to IR group. The levels of COX-2, malondialdehyde (MDA), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were lower in the NIM-50 and NIM-100 groups than in the IR group. Some histopathological changes seen in IR group. This findings were decreased in NIM-50 group and prevented in NIM-100 group.

CONCLUSION

Nimesulide prevented inflammation and oxidative stress. Our results suggest that nimesulide may be have a protective effect on testicular I/R injury.

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Authors+Show Affiliations

Polat EC
Department of Clinical Pharmacy, Faculty of Pharmacy, Erzincan University, 24100 Erzincan, Turkey.
Bozkurt AS
Department of Urology, Faculty of Medicine, Erzincan University, 24100 Erzincan, Turkey.
Keskin Cimen F
Department of Pathology, Faculty of Medicine, Erzincan University, 24100 Erzincan, Turkey.
Gulaboglu M
Department of Biochemistry, Faculty of Pharmacy, Ataturk University, 25240 Erzurum, Turkey.
Altuner D
Department of Pharmacology, Faculty of Medicine, Erzincan University Erzincan, Turkey. Electronic address: durdualtuner@hotmail.com.

MeSH

AnimalsAnti-Inflammatory Agents, Non-SteroidalAntioxidantsCyclooxygenase InhibitorsDose-Response Relationship, DrugInflammationMaleOxidative StressRatsRats, WistarReactive Oxygen SpeciesReperfusion InjurySpermatic Cord TorsionSulfonamides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30477960

Citation

Polat, Eyüp Can, et al. "The Investigation of the Protective Effects of Nimesulide On Experimental Testicular Ischemia-reperfusion Injury in Rats." Revista Internacional De Andrologia, vol. 18, no. 2, 2020, pp. 55-62.
Polat EC, Bozkurt AS, Keskin Cimen F, et al. The investigation of the protective effects of nimesulide on experimental testicular ischemia-reperfusion injury in rats. Rev Int Androl. 2020;18(2):55-62.
Polat, E. C., Bozkurt, A. S., Keskin Cimen, F., Gulaboglu, M., & Altuner, D. (2020). The investigation of the protective effects of nimesulide on experimental testicular ischemia-reperfusion injury in rats. Revista Internacional De Andrologia, 18(2), 55-62. https://doi.org/10.1016/j.androl.2018.08.001
Polat EC, et al. The Investigation of the Protective Effects of Nimesulide On Experimental Testicular Ischemia-reperfusion Injury in Rats. Rev Int Androl. 2020 Apr - Jun;18(2):55-62. PubMed PMID: 30477960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The investigation of the protective effects of nimesulide on experimental testicular ischemia-reperfusion injury in rats. AU - Polat,Eyüp Can, AU - Bozkurt,Ali Seydi, AU - Keskin Cimen,Ferda, AU - Gulaboglu,Mine, AU - Altuner,Durdu, Y1 - 2018/11/23/ PY - 2018/02/08/received PY - 2018/08/13/revised PY - 2018/08/31/accepted PY - 2018/11/28/pubmed PY - 2021/10/8/medline PY - 2018/11/28/entrez KW - Ischemia/reperfusion KW - Isquemia y reperfusión KW - Nimesulida KW - Nimesulide KW - Testis KW - Testículo SP - 55 EP - 62 JF - Revista internacional de andrologia JO - Rev Int Androl VL - 18 IS - 2 N2 - INTRODUCTION AND OBJECTIVES: Testicular ischemia/reperfusion (I/R) injury develops after torsion and following detorsion of the testis. Reactive oxygen species were produced and oxidative damage begins to occur due to I/R process. Nimesulide, which is a specific cyclooxygenase-2 inhibitor drug, have antioxidant, antiinflammatory, analgesics and antipyretic effects. We aimed to investigate biochemically and histopathologically effect of nimesulide on testis I/R injury in rats induced by the testicular torsion-detorsion. MATERIAL AND METHODS: In this study, 24 albino Wistar male rats were divided into four groups (6 rats in each group): ischemia/reperfusion applied+50mg/kg nimesulide administrated (NIM-50), ischemia/reperfusion applied+100mg/kg nimesulide administrated (NIM-100), ischemia/reperfusion applied (IR) and Sham surgery (SS) groups. Nimesulide was administered to NIM-50 and NIM-100 groups at the 50mg/kg and 100mg/kg doses before 2h applied I/R procedures. The IR group were applied only I/R procedures, no drug treatment was applied. Animals were sacrificed under high dose anesthesia and left testes were extracted. Testes were examined biochemically and histopathologically. RESULTS: Total glutathione (tGSH) and cyclooxygenase-1 (COX-1) levels were increased in the NIM-50 and NIM-100 groups compared to IR group. The levels of COX-2, malondialdehyde (MDA), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were lower in the NIM-50 and NIM-100 groups than in the IR group. Some histopathological changes seen in IR group. This findings were decreased in NIM-50 group and prevented in NIM-100 group. CONCLUSION: Nimesulide prevented inflammation and oxidative stress. Our results suggest that nimesulide may be have a protective effect on testicular I/R injury. SN - 1698-0409 UR - https://www.unboundmedicine.com/medline/citation/30477960/The_investigation_of_the_protective_effects_of_nimesulide_on_experimental_testicular_ischemia_reperfusion_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1698-031X(18)30078-5 DB - PRIME DP - Unbound Medicine ER -