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Detection and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Bulk Tank Milk of Cows, Sheep, and Goats.
Foodborne Pathog Dis. 2019 01; 16(1):68-73.FP

Abstract

This study is aimed at detecting and characterizing methicillin-resistant Staphylococcus aureus (MRSA) from bulk tank milk samples of cows, sheep, and goats collected from dairy farms in the Czech Republic. All MRSA isolates were identified using PCR detection of the Staphylococcus aureus-specific fragment SA442 and mecA gene. The staphylococcal chromosomal cassettes mec (SCCmec), spa, and multilocus sequence types (MLST) were determined. The presence of genes encoding enterotoxins (ses), Panton-Valentine leukocidin (pvl), exfoliative toxins A, B (eta, etb), and toxic shock syndrome toxin (tst) were assessed. To differentiate human and animal origin, the presence of staphylokinase (sak) gene, ϕSa3 prophage, and susceptibility to tetracycline was tested. Out of 49 bulk tank milk samples examined, 14 (28.6%) were MRSA-positive. Eleven positive samples came from cow's milk (38%) and the remaining three from goat's milk (33%). All samples of ewe's milk were negative. In MRSA isolates three sequence types containing seven spa types were identified. Twelve isolates (85.7%) belonged to ST398 spa types t011/SCCmec IVa, t011/SCCmec V, t034/SCCmec V, t1456/SCCmec IVa, t1255/SCCmec V, and t2346/SCCmec V. Another two isolates belonged to ST5/t3598/SCCmec IVa and ST8/t064/SCCmec IVNT. In six isolates, one or more ses genes (seb, sed, seg, sei, and sej) were confirmed. One isolate from cow's milk harbored the tst gene. Another two isolates (ST398/t1456/SCCmec IVa and ST5/t3598/SCCmec IVa) harbored the sak gene and ϕSa3 prophage, and the latter was the only tetracycline-susceptible isolate in this study. However, none of the isolates was positive for pvl or eta, etb. These results suggest that there is the wide geographical spread of ST398 across different regions of the Czech Republic with no host preference among dairy cattle and goats. Therefore, when evaluating the occupational and foodborne risks, MRSA carriage and infection should be taken into account.

Authors+Show Affiliations

1 Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic. 2 Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.1 Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic.1 Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic.1 Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic.1 Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30481051

Citation

Tegegne, Henok Ayalew, et al. "Detection and Molecular Characterization of Methicillin-Resistant Staphylococcus Aureus Isolated From Bulk Tank Milk of Cows, Sheep, and Goats." Foodborne Pathogens and Disease, vol. 16, no. 1, 2019, pp. 68-73.
Tegegne HA, Florianová M, Gelbíčová T, et al. Detection and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Bulk Tank Milk of Cows, Sheep, and Goats. Foodborne Pathog Dis. 2019;16(1):68-73.
Tegegne, H. A., Florianová, M., Gelbíčová, T., Karpíšková, R., & Koláčková, I. (2019). Detection and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Bulk Tank Milk of Cows, Sheep, and Goats. Foodborne Pathogens and Disease, 16(1), 68-73. https://doi.org/10.1089/fpd.2018.2511
Tegegne HA, et al. Detection and Molecular Characterization of Methicillin-Resistant Staphylococcus Aureus Isolated From Bulk Tank Milk of Cows, Sheep, and Goats. Foodborne Pathog Dis. 2019;16(1):68-73. PubMed PMID: 30481051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Bulk Tank Milk of Cows, Sheep, and Goats. AU - Tegegne,Henok Ayalew, AU - Florianová,Martina, AU - Gelbíčová,Tereza, AU - Karpíšková,Renáta, AU - Koláčková,Ivana, Y1 - 2018/11/27/ PY - 2018/11/28/pubmed PY - 2019/12/19/medline PY - 2018/11/28/entrez KW - type KW - MLST KW - MRSA KW - ST398 KW - chromosomal cassette KW - dairy farm SP - 68 EP - 73 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 16 IS - 1 N2 - This study is aimed at detecting and characterizing methicillin-resistant Staphylococcus aureus (MRSA) from bulk tank milk samples of cows, sheep, and goats collected from dairy farms in the Czech Republic. All MRSA isolates were identified using PCR detection of the Staphylococcus aureus-specific fragment SA442 and mecA gene. The staphylococcal chromosomal cassettes mec (SCCmec), spa, and multilocus sequence types (MLST) were determined. The presence of genes encoding enterotoxins (ses), Panton-Valentine leukocidin (pvl), exfoliative toxins A, B (eta, etb), and toxic shock syndrome toxin (tst) were assessed. To differentiate human and animal origin, the presence of staphylokinase (sak) gene, ϕSa3 prophage, and susceptibility to tetracycline was tested. Out of 49 bulk tank milk samples examined, 14 (28.6%) were MRSA-positive. Eleven positive samples came from cow's milk (38%) and the remaining three from goat's milk (33%). All samples of ewe's milk were negative. In MRSA isolates three sequence types containing seven spa types were identified. Twelve isolates (85.7%) belonged to ST398 spa types t011/SCCmec IVa, t011/SCCmec V, t034/SCCmec V, t1456/SCCmec IVa, t1255/SCCmec V, and t2346/SCCmec V. Another two isolates belonged to ST5/t3598/SCCmec IVa and ST8/t064/SCCmec IVNT. In six isolates, one or more ses genes (seb, sed, seg, sei, and sej) were confirmed. One isolate from cow's milk harbored the tst gene. Another two isolates (ST398/t1456/SCCmec IVa and ST5/t3598/SCCmec IVa) harbored the sak gene and ϕSa3 prophage, and the latter was the only tetracycline-susceptible isolate in this study. However, none of the isolates was positive for pvl or eta, etb. These results suggest that there is the wide geographical spread of ST398 across different regions of the Czech Republic with no host preference among dairy cattle and goats. Therefore, when evaluating the occupational and foodborne risks, MRSA carriage and infection should be taken into account. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/30481051/Detection_and_Molecular_Characterization_of_Methicillin_Resistant_Staphylococcus_aureus_Isolated_from_Bulk_Tank_Milk_of_Cows_Sheep_and_Goats_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2018.2511?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -