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Kinetic modeling and sensitivity analysis for higher ethanol production in self-cloning xylose-using Saccharomyces cerevisiae.
J Biosci Bioeng. 2019 May; 127(5):563-569.JB

Abstract

We constructed a xylose-utilizing Saccharomyces cerevisiae strain using endogenous xylose-assimilating genes (strain K7-XYL). Such self-cloning yeast is expected to make a great contribution to cost reduction of ethanol production processes. However, it is difficult to modify self-cloning yeast for optimal performance because the available gene source is limited. To improve the ethanol productivity of our self-cloning yeast, a kinetic model of ethanol production was constructed and sensitivity analysis was performed. Alcohol dehydrogenase (ADH1) was identified as a metabolic bottleneck reaction in the ethanol production pathway. An ADH1 overexpression strain (K7-XYL-ADH1) was constructed and evaluated in YP (yeast extract 10 g/L, peptone 20 g/L) medium containing 50 g/L xylose as the sole carbon source. Strain K7-XYL-ADH1 showed higher ethanol productivity (13.8 g/L) than strain K7-XYL (12.5 g/L). Then, K7-XYL-ADH1 was evaluated in YP medium containing 80 g/L glucose and 50 g/L xylose; however, the ethanol productivity did not change relative to that of K7-XYL (K7-XYL 46.3 g/L, K7-XYL-ADH1 45.9 g/L). We presumed that due to the presence of glucose, the internal redox balance of the cells had changed. On culturing in an aerated 5-L jar fermentor to change the internal redox balance of cells, strain K7-XYL-ADH1 showed higher ethanol productivity than K7-XYL (K7-XYL 45.0 g/L, K7-XYL-ADH1 49.4 g/L). Our results confirmed that ADH1 was a metabolic bottleneck in the ethanol production pathway. By eliminating the bottleneck, self-cloning yeast showed almost the same ethanol productivity as genetically modified yeast.

Authors+Show Affiliations

Laboratory of Synthetic Biology, Graduate School of Bioresource and Bioenvironmental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Biofuel R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JXTG Nippon Oil & Energy Corporation, 8 Chidoricho, Naka-ku, Yokohama 231-0815, Japan. Electronic address: fukuda@brs.kyushu-u.ac.jp.Laboratory of Synthetic Biology, Graduate School of Bioresource and Bioenvironmental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: kuriya@people.kobe-u.ac.jp.Biofuel R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JXTG Nippon Oil & Energy Corporation, 8 Chidoricho, Naka-ku, Yokohama 231-0815, Japan. Electronic address: jin.konishi@jxtg.com.Biofuel R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JXTG Nippon Oil & Energy Corporation, 8 Chidoricho, Naka-ku, Yokohama 231-0815, Japan. Electronic address: kozue.mutaguchi@jxtg.com.Biofuel R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JXTG Nippon Oil & Energy Corporation, 8 Chidoricho, Naka-ku, Yokohama 231-0815, Japan. Electronic address: takeshi.uemura.922@jxtg.com.Metabolic Profiling Research Group, Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: daipon@agr.kyushu-u.ac.jp.Laboratory of Synthetic Biology, Graduate School of Systems Life Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: okahon@brs.kyushu-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30482500

Citation

Fukuda, Akira, et al. "Kinetic Modeling and Sensitivity Analysis for Higher Ethanol Production in Self-cloning Xylose-using Saccharomyces Cerevisiae." Journal of Bioscience and Bioengineering, vol. 127, no. 5, 2019, pp. 563-569.
Fukuda A, Kuriya Y, Konishi J, et al. Kinetic modeling and sensitivity analysis for higher ethanol production in self-cloning xylose-using Saccharomyces cerevisiae. J Biosci Bioeng. 2019;127(5):563-569.
Fukuda, A., Kuriya, Y., Konishi, J., Mutaguchi, K., Uemura, T., Miura, D., & Okamoto, M. (2019). Kinetic modeling and sensitivity analysis for higher ethanol production in self-cloning xylose-using Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 127(5), 563-569. https://doi.org/10.1016/j.jbiosc.2018.10.020
Fukuda A, et al. Kinetic Modeling and Sensitivity Analysis for Higher Ethanol Production in Self-cloning Xylose-using Saccharomyces Cerevisiae. J Biosci Bioeng. 2019;127(5):563-569. PubMed PMID: 30482500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kinetic modeling and sensitivity analysis for higher ethanol production in self-cloning xylose-using Saccharomyces cerevisiae. AU - Fukuda,Akira, AU - Kuriya,Yuki, AU - Konishi,Jin, AU - Mutaguchi,Kozue, AU - Uemura,Takeshi, AU - Miura,Daisuke, AU - Okamoto,Masahiro, Y1 - 2018/11/24/ PY - 2018/09/17/received PY - 2018/10/20/revised PY - 2018/10/25/accepted PY - 2018/11/30/pubmed PY - 2019/6/18/medline PY - 2018/11/29/entrez KW - Ethanol production KW - Kinetic modeling KW - Metabolic bottleneck KW - Saccharomyces cerevisiae KW - Self-cloning KW - Xylose SP - 563 EP - 569 JF - Journal of bioscience and bioengineering JO - J Biosci Bioeng VL - 127 IS - 5 N2 - We constructed a xylose-utilizing Saccharomyces cerevisiae strain using endogenous xylose-assimilating genes (strain K7-XYL). Such self-cloning yeast is expected to make a great contribution to cost reduction of ethanol production processes. However, it is difficult to modify self-cloning yeast for optimal performance because the available gene source is limited. To improve the ethanol productivity of our self-cloning yeast, a kinetic model of ethanol production was constructed and sensitivity analysis was performed. Alcohol dehydrogenase (ADH1) was identified as a metabolic bottleneck reaction in the ethanol production pathway. An ADH1 overexpression strain (K7-XYL-ADH1) was constructed and evaluated in YP (yeast extract 10 g/L, peptone 20 g/L) medium containing 50 g/L xylose as the sole carbon source. Strain K7-XYL-ADH1 showed higher ethanol productivity (13.8 g/L) than strain K7-XYL (12.5 g/L). Then, K7-XYL-ADH1 was evaluated in YP medium containing 80 g/L glucose and 50 g/L xylose; however, the ethanol productivity did not change relative to that of K7-XYL (K7-XYL 46.3 g/L, K7-XYL-ADH1 45.9 g/L). We presumed that due to the presence of glucose, the internal redox balance of the cells had changed. On culturing in an aerated 5-L jar fermentor to change the internal redox balance of cells, strain K7-XYL-ADH1 showed higher ethanol productivity than K7-XYL (K7-XYL 45.0 g/L, K7-XYL-ADH1 49.4 g/L). Our results confirmed that ADH1 was a metabolic bottleneck in the ethanol production pathway. By eliminating the bottleneck, self-cloning yeast showed almost the same ethanol productivity as genetically modified yeast. SN - 1347-4421 UR - https://www.unboundmedicine.com/medline/citation/30482500/Kinetic_modeling_and_sensitivity_analysis_for_higher_ethanol_production_in_self_cloning_xylose_using_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -