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Identification of a Novel Semi-Dominant Spotted-Leaf Mutant with Enhanced Resistance to Xanthomonas oryzae pv. oryzae in Rice.
Int J Mol Sci. 2018 Nov 27; 19(12)IJ

Abstract

Many spotted-leaf mutants show enhanced disease resistance to multiple pathogen attacks; however, the mechanisms are largely unknown. Here, we reported a novel semi-dominant spotted-leaf mutant 24 (spl24) obtained from an ethyl methane sulfonate (EMS)-induced IR64 mutant bank. spl24 developed tiny brown lesions on the leaf tip and spread down gradually to the leaf base as well as the sheath at the early heading stage. The performances of major agronomic traits such as the plant height, panicle length, number of panicles/plant, and 1000-grain weight were significantly altered in spl24 when compared to the wild-type IR64. Furthermore, spl24 exhibited a premature senescing phenotype with degeneration of nuclear acids, significantly reduced soluble protein content, increased level of malonaldehyde (MDA), and lowered activities of reactive oxygen species (ROS) scavenging enzymes. Disease evaluation indicated that spl24 showed enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae, the causal pathogen of bacterial leaf blight in rice, with elevated expression of pathogenesis-related genes, salicylic acid (SA) signaling pathway-associated genes revealed by real-time quantitative PCR and high-throughput RNA sequencing analysis. Genetic analysis and gene mapping indicated that the lesion mimic phenotype was controlled by a novel semi-dominant nuclear gene. The mutation, tentatively termed as OsSPL24, was in a 110 kb region flanked by markers Indel-33 and Indel-12 in chromosome 11. Together, our data suggest that spl24 is a novel lesion mimic mutant with enhanced innate immunity and would facilitate the isolation and functional characterization of the target gene.

Authors+Show Affiliations

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. chenzheng7@126.com. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. chenzheng7@126.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. chenting218@163.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. atulsathe@163.com.College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. yqhe@mail.hzau.edu.cn.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. zhangxiaobo@caas.cn.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. wujianli@caas.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30486418

Citation

Chen, Zheng, et al. "Identification of a Novel Semi-Dominant Spotted-Leaf Mutant With Enhanced Resistance to Xanthomonas Oryzae Pv. Oryzae in Rice." International Journal of Molecular Sciences, vol. 19, no. 12, 2018.
Chen Z, Chen T, Sathe AP, et al. Identification of a Novel Semi-Dominant Spotted-Leaf Mutant with Enhanced Resistance to Xanthomonas oryzae pv. oryzae in Rice. Int J Mol Sci. 2018;19(12).
Chen, Z., Chen, T., Sathe, A. P., He, Y., Zhang, X. B., & Wu, J. L. (2018). Identification of a Novel Semi-Dominant Spotted-Leaf Mutant with Enhanced Resistance to Xanthomonas oryzae pv. oryzae in Rice. International Journal of Molecular Sciences, 19(12). https://doi.org/10.3390/ijms19123766
Chen Z, et al. Identification of a Novel Semi-Dominant Spotted-Leaf Mutant With Enhanced Resistance to Xanthomonas Oryzae Pv. Oryzae in Rice. Int J Mol Sci. 2018 Nov 27;19(12) PubMed PMID: 30486418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of a Novel Semi-Dominant Spotted-Leaf Mutant with Enhanced Resistance to Xanthomonas oryzae pv. oryzae in Rice. AU - Chen,Zheng, AU - Chen,Ting, AU - Sathe,Atul Prakash, AU - He,Yuqing, AU - Zhang,Xiao-Bo, AU - Wu,Jian-Li, Y1 - 2018/11/27/ PY - 2018/10/19/received PY - 2018/11/15/revised PY - 2018/11/22/accepted PY - 2018/11/30/entrez PY - 2018/11/30/pubmed PY - 2019/3/7/medline KW - bacterial leaf blight KW - defense response KW - phytohormone KW - rice KW - spotted-leaf JF - International journal of molecular sciences JO - Int J Mol Sci VL - 19 IS - 12 N2 - Many spotted-leaf mutants show enhanced disease resistance to multiple pathogen attacks; however, the mechanisms are largely unknown. Here, we reported a novel semi-dominant spotted-leaf mutant 24 (spl24) obtained from an ethyl methane sulfonate (EMS)-induced IR64 mutant bank. spl24 developed tiny brown lesions on the leaf tip and spread down gradually to the leaf base as well as the sheath at the early heading stage. The performances of major agronomic traits such as the plant height, panicle length, number of panicles/plant, and 1000-grain weight were significantly altered in spl24 when compared to the wild-type IR64. Furthermore, spl24 exhibited a premature senescing phenotype with degeneration of nuclear acids, significantly reduced soluble protein content, increased level of malonaldehyde (MDA), and lowered activities of reactive oxygen species (ROS) scavenging enzymes. Disease evaluation indicated that spl24 showed enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae, the causal pathogen of bacterial leaf blight in rice, with elevated expression of pathogenesis-related genes, salicylic acid (SA) signaling pathway-associated genes revealed by real-time quantitative PCR and high-throughput RNA sequencing analysis. Genetic analysis and gene mapping indicated that the lesion mimic phenotype was controlled by a novel semi-dominant nuclear gene. The mutation, tentatively termed as OsSPL24, was in a 110 kb region flanked by markers Indel-33 and Indel-12 in chromosome 11. Together, our data suggest that spl24 is a novel lesion mimic mutant with enhanced innate immunity and would facilitate the isolation and functional characterization of the target gene. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/30486418/Identification_of_a_Novel_Semi_Dominant_Spotted_Leaf_Mutant_with_Enhanced_Resistance_to_Xanthomonas_oryzae_pv__oryzae_in_Rice_ L2 - https://www.mdpi.com/resolver?pii=ijms19123766 DB - PRIME DP - Unbound Medicine ER -