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In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa.
BMC Complement Altern Med. 2018 Nov 29; 18(1):315.BC

Abstract

BACKGROUND

Compounds having both anticancer and antimicrobial activity have promising therapeutic potential due to their selective cytotoxicity and their potential to reduce the occurrence of bacterial and fungal infections in immune-compromised cancer patients. In our quest to find new antimicrobial agents with potent anticancer activity, the biological potential of leaves from the three medicinal plants Centella asiatica, Warburgia salutaris and Curtisia dentata as used by Zulu traditional healers for the treatment of cancer is investigated.

METHODS

Extracts were assayed for antibacterial activity using the agar well diffusion and micro plate dilution assay. In addition, minimum bactericidal concentrations (MBC), lactate dehydrogenase (LDH) release assay and rhodamine 6G intake assay were used to ascertain the antibacterial activity. The cytotoxic effects of the plant extracts were determined using tetrazolium-based colorimetric (MTT) cell proliferation assay against MCF-7, human colorectal carcinoma cells (Caco-2), A549 and HeLa cancerous cell lines.

RESULTS

The acetone extracts from Waburgia salutaris revealed noteworthy anti-proliferative effect yielding IC50 value of 34.15 μg/ml against MCF-7 cell line, while acetone extract from Curtisia dentata significantly (P ≤ 0.05) revealed promising IC50 values of 41.55, 45.13, 57.35 and 43.24 μg/ml against A549, HeLa, CaCo-2 and MCF-7 cell lines. The extracts further revealed a broad-spectrum antibacterial activity against bacterial strains used in the study. An acetone extract from W. salutaris revealed the highest zone of inhibition and the lowest minimum inhibitory concentration (MIC) of 21.0 mm and 0.16 mg/ml respectively against Staphylococcus aureus. Methanol extract from W. salutaris and ethyl acetate extract from C. dentata revealed 53% inhibition of R6G inside the cell against Staphylococcus aureus and Escherichia coli respectively in a cytosolic lactate dehydrogenase assay, suggesting that the mode of action of such extracts may be through efflux pump.

CONCLUSIONS

Overall, the extracts had good antibacterial activity and anti-proliferative effects against selected cancerous cell lines. Given the good antibacterial activity of the extracts the plants may act as an immune booster and prevent infection in immunosuppressed cancer patients. This is further supported by the plants' anti-proliferative potential, bacteriostatic, bactericidal properties and also their ability to block bacterial efflux pump systems.

Authors+Show Affiliations

Research, Innovation and Engagements Portfolio, Mangosuthu University of Technology, Durban, 4031, South Africa. soyingbe@yahoo.com.University of South Africa, College of Agriculture and Environmental Sciences Laboratories, Private Bag X06, Florida, Johannesburg, 0710, South Africa.Research, Innovation and Engagements Portfolio, Mangosuthu University of Technology, Durban, 4031, South Africa.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30497461

Citation

Soyingbe, Oluwagbemiga Sewanu, et al. "In Vitro Antibacterial and Cytotoxic Activity of Leaf Extracts of Centella Asiatica (L.) Urb, Warburgia Salutaris (Bertol. F.) Chiov and Curtisia Dentata (Burm. F.) C.A.Sm - Medicinal Plants Used in South Africa." BMC Complementary and Alternative Medicine, vol. 18, no. 1, 2018, p. 315.
Soyingbe OS, Mongalo NI, Makhafola TJ. In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa. BMC Complement Altern Med. 2018;18(1):315.
Soyingbe, O. S., Mongalo, N. I., & Makhafola, T. J. (2018). In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa. BMC Complementary and Alternative Medicine, 18(1), 315. https://doi.org/10.1186/s12906-018-2378-3
Soyingbe OS, Mongalo NI, Makhafola TJ. In Vitro Antibacterial and Cytotoxic Activity of Leaf Extracts of Centella Asiatica (L.) Urb, Warburgia Salutaris (Bertol. F.) Chiov and Curtisia Dentata (Burm. F.) C.A.Sm - Medicinal Plants Used in South Africa. BMC Complement Altern Med. 2018 Nov 29;18(1):315. PubMed PMID: 30497461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa. AU - Soyingbe,Oluwagbemiga Sewanu, AU - Mongalo,Nkoana Ishmael, AU - Makhafola,Tshepiso Jan, Y1 - 2018/11/29/ PY - 2018/07/12/received PY - 2018/11/16/accepted PY - 2018/12/1/entrez PY - 2018/12/1/pubmed PY - 2019/1/12/medline KW - Anti-proliferative effect KW - Antibacterial activity KW - Ethno-medicine KW - Immunosuppressed KW - Medicinal plants SP - 315 EP - 315 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 18 IS - 1 N2 - BACKGROUND: Compounds having both anticancer and antimicrobial activity have promising therapeutic potential due to their selective cytotoxicity and their potential to reduce the occurrence of bacterial and fungal infections in immune-compromised cancer patients. In our quest to find new antimicrobial agents with potent anticancer activity, the biological potential of leaves from the three medicinal plants Centella asiatica, Warburgia salutaris and Curtisia dentata as used by Zulu traditional healers for the treatment of cancer is investigated. METHODS: Extracts were assayed for antibacterial activity using the agar well diffusion and micro plate dilution assay. In addition, minimum bactericidal concentrations (MBC), lactate dehydrogenase (LDH) release assay and rhodamine 6G intake assay were used to ascertain the antibacterial activity. The cytotoxic effects of the plant extracts were determined using tetrazolium-based colorimetric (MTT) cell proliferation assay against MCF-7, human colorectal carcinoma cells (Caco-2), A549 and HeLa cancerous cell lines. RESULTS: The acetone extracts from Waburgia salutaris revealed noteworthy anti-proliferative effect yielding IC50 value of 34.15 μg/ml against MCF-7 cell line, while acetone extract from Curtisia dentata significantly (P ≤ 0.05) revealed promising IC50 values of 41.55, 45.13, 57.35 and 43.24 μg/ml against A549, HeLa, CaCo-2 and MCF-7 cell lines. The extracts further revealed a broad-spectrum antibacterial activity against bacterial strains used in the study. An acetone extract from W. salutaris revealed the highest zone of inhibition and the lowest minimum inhibitory concentration (MIC) of 21.0 mm and 0.16 mg/ml respectively against Staphylococcus aureus. Methanol extract from W. salutaris and ethyl acetate extract from C. dentata revealed 53% inhibition of R6G inside the cell against Staphylococcus aureus and Escherichia coli respectively in a cytosolic lactate dehydrogenase assay, suggesting that the mode of action of such extracts may be through efflux pump. CONCLUSIONS: Overall, the extracts had good antibacterial activity and anti-proliferative effects against selected cancerous cell lines. Given the good antibacterial activity of the extracts the plants may act as an immune booster and prevent infection in immunosuppressed cancer patients. This is further supported by the plants' anti-proliferative potential, bacteriostatic, bactericidal properties and also their ability to block bacterial efflux pump systems. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/30497461/In_vitro_antibacterial_and_cytotoxic_activity_of_leaf_extracts_of_Centella_asiatica__L___Urb_Warburgia_salutaris__Bertol__F___Chiov_and_Curtisia_dentata__Burm__F___C_A_Sm___medicinal_plants_used_in_South_Africa_ DB - PRIME DP - Unbound Medicine ER -