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Controlled release of lawsone from polycaprolactone/gelatin electrospun nano fibers for skin tissue regeneration.
Int J Biol Macromol 2019; 124:478-491IJ

Abstract

In this study, we aimed to evaluate the role of electrospun nanofibers containing lawsone (2-hydroxy-1,4-naphthoquinone) in wound healing. Different concentrations of lawsone (0.5%, 1%, 1.5%) were electrospun in polycaprolactone-gelatin (PCL/Gel) polymers in core-shell architecture. Nanofibers were characterized using scanning electron microscopy (SEM), Transmission electron microscopy (TEM). Coaxial electrospinning of PCL/Gel/lawsone (PCL/Gel/Law) scaffolds prolonged the release of lawsone over a period of 20 days. In vitro bioactivity of fibers on human gingiva fibroblast cells (HGF) was evaluated by MTT assay. The PCL/Gel/Law 1% increased cell attachment and proliferation significantly. Additionally, the in vitro gene expression of transforming growth factor β (TGF-B1), collagen (COL1) and epidermal growth factor (EGF) was monitored using RT-qPCR technique, which there was significant increase in TGF-B1 and COL1 gene expression in PCL/Gel/Law 0.5% and 1% mats. In vivo wound healing activity of lawsone loaded scaffolds in rat wound model revealed that the PCL/Gel/Law 1% had the highest impact on healing by increasing re-epithelialization of the wound after 14 days. It can be inferred that lawsone 1% incorporation in the core of PCL/Gel electrospun nanofibers has excellent characteristics and can be used as wound dressing patch in medicine.

Authors+Show Affiliations

Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: m.yaghoobi@kgut.ac.ir.Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30500508

Citation

Adeli-Sardou, Mahboobeh, et al. "Controlled Release of Lawsone From Polycaprolactone/gelatin Electrospun Nano Fibers for Skin Tissue Regeneration." International Journal of Biological Macromolecules, vol. 124, 2019, pp. 478-491.
Adeli-Sardou M, Yaghoobi MM, Torkzadeh-Mahani M, et al. Controlled release of lawsone from polycaprolactone/gelatin electrospun nano fibers for skin tissue regeneration. Int J Biol Macromol. 2019;124:478-491.
Adeli-Sardou, M., Yaghoobi, M. M., Torkzadeh-Mahani, M., & Dodel, M. (2019). Controlled release of lawsone from polycaprolactone/gelatin electrospun nano fibers for skin tissue regeneration. International Journal of Biological Macromolecules, 124, pp. 478-491. doi:10.1016/j.ijbiomac.2018.11.237.
Adeli-Sardou M, et al. Controlled Release of Lawsone From Polycaprolactone/gelatin Electrospun Nano Fibers for Skin Tissue Regeneration. Int J Biol Macromol. 2019 Mar 1;124:478-491. PubMed PMID: 30500508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Controlled release of lawsone from polycaprolactone/gelatin electrospun nano fibers for skin tissue regeneration. AU - Adeli-Sardou,Mahboobeh, AU - Yaghoobi,Mohammad Mehdi, AU - Torkzadeh-Mahani,Masoud, AU - Dodel,Masoumeh, Y1 - 2018/11/27/ PY - 2018/06/23/received PY - 2018/11/15/revised PY - 2018/11/26/accepted PY - 2018/12/1/pubmed PY - 2019/4/27/medline PY - 2018/12/1/entrez KW - Coaxial electrospinning KW - Gelatin KW - Lawsone KW - Polycaprolactone KW - Tissue engineering KW - Wound healing SP - 478 EP - 491 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 124 N2 - In this study, we aimed to evaluate the role of electrospun nanofibers containing lawsone (2-hydroxy-1,4-naphthoquinone) in wound healing. Different concentrations of lawsone (0.5%, 1%, 1.5%) were electrospun in polycaprolactone-gelatin (PCL/Gel) polymers in core-shell architecture. Nanofibers were characterized using scanning electron microscopy (SEM), Transmission electron microscopy (TEM). Coaxial electrospinning of PCL/Gel/lawsone (PCL/Gel/Law) scaffolds prolonged the release of lawsone over a period of 20 days. In vitro bioactivity of fibers on human gingiva fibroblast cells (HGF) was evaluated by MTT assay. The PCL/Gel/Law 1% increased cell attachment and proliferation significantly. Additionally, the in vitro gene expression of transforming growth factor β (TGF-B1), collagen (COL1) and epidermal growth factor (EGF) was monitored using RT-qPCR technique, which there was significant increase in TGF-B1 and COL1 gene expression in PCL/Gel/Law 0.5% and 1% mats. In vivo wound healing activity of lawsone loaded scaffolds in rat wound model revealed that the PCL/Gel/Law 1% had the highest impact on healing by increasing re-epithelialization of the wound after 14 days. It can be inferred that lawsone 1% incorporation in the core of PCL/Gel electrospun nanofibers has excellent characteristics and can be used as wound dressing patch in medicine. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/30500508/Controlled_release_of_lawsone_from_polycaprolactone/gelatin_electrospun_nano_fibers_for_skin_tissue_regeneration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)33120-9 DB - PRIME DP - Unbound Medicine ER -