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Analysis of Immunologic Function Changes in Lichen Planus After Clinical Treatment.
Med Sci Monit. 2018 Dec 02; 24:8716-8721.MS

Abstract

BACKGROUND

Lichen planus (LP) is a common chronic superficial skin lesion that causes chronic or sub-acute inflammatory disorders. LP can affect the oral cavity, skin, mucous membrane, and other body parts, and features include repeat attacks and long duration, leading to lower quality of life. This study aimed to analyze the changes of immunologic function before and after treatment of LP. MATERIAL AND

METHODS

Thirty cutaneous LP patients were selected. Peripheral blood was collected in the morning before and after treatment. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient method. Flow cytometry was used to detect T cell subpopulation CD4⁺ T cells and CD8⁺ T to calculate CD4⁺ T/CD8⁺ T ratio. Enzyme-linked immunosorbent assay (ELISA) was adopted to detect the helper T-cell (Th) factor IL-2, IFN-γ, IL-4, IL-6, IL-17, and IL-22 levels.

RESULTS

Compared with before treatment, the expressions of CD4⁺ T cells and CD8⁺ T cells were decreased, while the proportion of CD4⁺ T/CD8⁺ T were significantly elevated after treatment. IL-2 and IFN-γ secretion were markedly increased, whereas IL-4, IL-6, IL-17, and IL-22 were significantly reduced after treatment (P<0.05).

CONCLUSIONS

LP treatment reduces the distribution of CD4⁺ T cells and CD8⁺ T cells, and promotes the changes of Th1, Th2, and Th17 cytokines secretion.

Authors+Show Affiliations

Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, China (mainland).Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, China (mainland).Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, China (mainland).Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, China (mainland).Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, China (mainland).Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, China (mainland).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30504759

Citation

Qian, Hua, et al. "Analysis of Immunologic Function Changes in Lichen Planus After Clinical Treatment." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 24, 2018, pp. 8716-8721.
Qian H, Jiao L, Fan Z, et al. Analysis of Immunologic Function Changes in Lichen Planus After Clinical Treatment. Med Sci Monit. 2018;24:8716-8721.
Qian, H., Jiao, L., Fan, Z., Wang, L., Liu, B., & Miao, G. (2018). Analysis of Immunologic Function Changes in Lichen Planus After Clinical Treatment. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 8716-8721. https://doi.org/10.12659/MSM.910931
Qian H, et al. Analysis of Immunologic Function Changes in Lichen Planus After Clinical Treatment. Med Sci Monit. 2018 Dec 2;24:8716-8721. PubMed PMID: 30504759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Immunologic Function Changes in Lichen Planus After Clinical Treatment. AU - Qian,Hua, AU - Jiao,Liyan, AU - Fan,Zhixia, AU - Wang,Litao, AU - Liu,Baoguo, AU - Miao,Guoying, Y1 - 2018/12/02/ PY - 2018/12/4/entrez PY - 2018/12/7/pubmed PY - 2019/2/14/medline SP - 8716 EP - 8721 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med. Sci. Monit. VL - 24 N2 - BACKGROUND Lichen planus (LP) is a common chronic superficial skin lesion that causes chronic or sub-acute inflammatory disorders. LP can affect the oral cavity, skin, mucous membrane, and other body parts, and features include repeat attacks and long duration, leading to lower quality of life. This study aimed to analyze the changes of immunologic function before and after treatment of LP. MATERIAL AND METHODS Thirty cutaneous LP patients were selected. Peripheral blood was collected in the morning before and after treatment. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient method. Flow cytometry was used to detect T cell subpopulation CD4⁺ T cells and CD8⁺ T to calculate CD4⁺ T/CD8⁺ T ratio. Enzyme-linked immunosorbent assay (ELISA) was adopted to detect the helper T-cell (Th) factor IL-2, IFN-γ, IL-4, IL-6, IL-17, and IL-22 levels. RESULTS Compared with before treatment, the expressions of CD4⁺ T cells and CD8⁺ T cells were decreased, while the proportion of CD4⁺ T/CD8⁺ T were significantly elevated after treatment. IL-2 and IFN-γ secretion were markedly increased, whereas IL-4, IL-6, IL-17, and IL-22 were significantly reduced after treatment (P<0.05). CONCLUSIONS LP treatment reduces the distribution of CD4⁺ T cells and CD8⁺ T cells, and promotes the changes of Th1, Th2, and Th17 cytokines secretion. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/30504759/Analysis_of_Immunologic_Function_Changes_in_Lichen_Planus_After_Clinical_Treatment_ L2 - https://www.medscimonit.com/download/index/idArt/910931 DB - PRIME DP - Unbound Medicine ER -