Tags

Type your tag names separated by a space and hit enter

Low-linoleic acid diet and oestrogen enhance the conversion of α-linolenic acid into DHA through modification of conversion enzymes and transcription factors.
Br J Nutr. 2019 01; 121(2):137-145.BJ

Abstract

Conversion of α-linolenic acid (ALA) into the longer chain n-3 PUFA has been suggested to be affected by the dietary intake of linoleic acid (LA), but the mechanism is not well known. Therefore, the purpose of this study was to evaluate the effect of a low-LA diet with and without oestrogen on the fatty acid conversion enzymes and transcription factors. Rats were fed a modified American Institute of Nutrition-93G diet with 0% n-3 PUFA or ALA, containing low or high amounts of LA for 12 weeks. At 8 weeks, the rats were injected with maize oil with or without 17β-oestradiol-3-benzoate (E) at constant intervals for the remaining 3 weeks. Both the low-LA diet and E significantly increased the hepatic expressions of PPAR-α, fatty acid desaturase (FADS) 2, elongase of very long chain fatty acids 2 (ELOVL2) and ELOVL5 but decreased sterol regulatory element binding protein 1. The low-LA diet, but not E, increased the hepatic expression of FADS1, and E increased the hepatic expression of oestrogen receptor-α and β. The low-LA diet and E had synergic effects on serum and liver levels of DHA and on the hepatic expression of PPAR-α. In conclusion, the low-LA diet and oestrogen increased the conversion of ALA into DHA by upregulating the elongases and desaturases of fatty acids through regulating the expression of transcription factors. The low-LA diet and E had a synergic effect on serum and liver levels of DHA through increasing the expression of PPAR-α.

Authors+Show Affiliations

Department of Food and Nutrition,Hanyang University,222 Wangsimni-ro, Seongdong-gu, Seoul 04763,Republic of Korea.Department of Food and Nutrition,Hanyang University,222 Wangsimni-ro, Seongdong-gu, Seoul 04763,Republic of Korea.Department of Food and Nutrition,Hanyang University,222 Wangsimni-ro, Seongdong-gu, Seoul 04763,Republic of Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30507367

Citation

Kim, Donghee, et al. "Low-linoleic Acid Diet and Oestrogen Enhance the Conversion of Α-linolenic Acid Into DHA Through Modification of Conversion Enzymes and Transcription Factors." The British Journal of Nutrition, vol. 121, no. 2, 2019, pp. 137-145.
Kim D, Choi JE, Park Y. Low-linoleic acid diet and oestrogen enhance the conversion of α-linolenic acid into DHA through modification of conversion enzymes and transcription factors. Br J Nutr. 2019;121(2):137-145.
Kim, D., Choi, J. E., & Park, Y. (2019). Low-linoleic acid diet and oestrogen enhance the conversion of α-linolenic acid into DHA through modification of conversion enzymes and transcription factors. The British Journal of Nutrition, 121(2), 137-145. https://doi.org/10.1017/S0007114518003252
Kim D, Choi JE, Park Y. Low-linoleic Acid Diet and Oestrogen Enhance the Conversion of Α-linolenic Acid Into DHA Through Modification of Conversion Enzymes and Transcription Factors. Br J Nutr. 2019;121(2):137-145. PubMed PMID: 30507367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Low-linoleic acid diet and oestrogen enhance the conversion of α-linolenic acid into DHA through modification of conversion enzymes and transcription factors. AU - Kim,Donghee, AU - Choi,Jeong-Eun, AU - Park,Yongsoon, Y1 - 2018/12/03/ PY - 2018/12/7/pubmed PY - 2019/12/21/medline PY - 2018/12/4/entrez KW - α-Linolenic acid KW - ACOX1 acyl-coenzyme A oxidase 1 KW - ALA α-linolenic acid KW - E 17β-oestradiol-3-benzoate KW - ELOVL elongase of very long fatty acid KW - ER oestrogen receptor KW - FADS fatty acid desaturase KW - LA linoleic acid KW - SREBP1 sterol regulatory element binding protein 1 KW - Conversion enzymes KW - Low-linoleic acid diet KW - Oestrogen SP - 137 EP - 145 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 121 IS - 2 N2 - Conversion of α-linolenic acid (ALA) into the longer chain n-3 PUFA has been suggested to be affected by the dietary intake of linoleic acid (LA), but the mechanism is not well known. Therefore, the purpose of this study was to evaluate the effect of a low-LA diet with and without oestrogen on the fatty acid conversion enzymes and transcription factors. Rats were fed a modified American Institute of Nutrition-93G diet with 0% n-3 PUFA or ALA, containing low or high amounts of LA for 12 weeks. At 8 weeks, the rats were injected with maize oil with or without 17β-oestradiol-3-benzoate (E) at constant intervals for the remaining 3 weeks. Both the low-LA diet and E significantly increased the hepatic expressions of PPAR-α, fatty acid desaturase (FADS) 2, elongase of very long chain fatty acids 2 (ELOVL2) and ELOVL5 but decreased sterol regulatory element binding protein 1. The low-LA diet, but not E, increased the hepatic expression of FADS1, and E increased the hepatic expression of oestrogen receptor-α and β. The low-LA diet and E had synergic effects on serum and liver levels of DHA and on the hepatic expression of PPAR-α. In conclusion, the low-LA diet and oestrogen increased the conversion of ALA into DHA by upregulating the elongases and desaturases of fatty acids through regulating the expression of transcription factors. The low-LA diet and E had a synergic effect on serum and liver levels of DHA through increasing the expression of PPAR-α. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/30507367/Low_linoleic_acid_diet_and_oestrogen_enhance_the_conversion_of_α_linolenic_acid_into_DHA_through_modification_of_conversion_enzymes_and_transcription_factors_ L2 - https://www.cambridge.org/core/product/identifier/S0007114518003252/type/journal_article DB - PRIME DP - Unbound Medicine ER -