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Sputum Moraxella catarrhalis strains exhibit diversity within and between COPD subjects.
Int J Chron Obstruct Pulmon Dis 2018; 13:3663-3667IJ

Abstract

Purpose

Moraxella catarrhalis is implicated in the pathogenesis of some COPD exacerbations. We sought to investigate whether the M. catarrhalis strain is variable between COPD subjects; that an exacerbation is associated with acquisition of a new strain and that certain strains are more commonly associated with exacerbations.

Patients and methods

Sputum samples were collected at stable and exacerbation visits from COPD subjects from a single center as part of the COPDMAP consortium. Samples identified as M. catarrhalis positive by qPCR were recultured in liquid cultures grown to extract genomic DNA; underwent Illumina MiSeq and bacterial genome sequences were de novo assembled and Multi Locus Sequence Type (MLST) was determined.

Results

Thirty-five samples were obtained from 18 subjects. These included 13 stable and 22 exacerbation samples. The diversity between samples was very large with 25 different M. catarrhalis MLSTs being identified out of the 35 samples of which 12 MSLTs have not been described previously. Change and persistence of M. catarrhalis strain were observed between stable visits, from stable to exacerbation and vice-a-versa, and between exacerbation visits.

Conclusion

Sputum M. catarrhalis strains exhibit marked diversity within and between COPD subjects. Acquisition of a new strain is common between stable and exacerbation events such that no strain is specifically associated with an exacerbation.

Authors+Show Affiliations

Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK, ceb17@le.ac.uk.Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK, ceb17@le.ac.uk.Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK, ceb17@le.ac.uk.Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK, ceb17@le.ac.uk.Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK, ceb17@le.ac.uk.Department of Genetics, University of Leicester, Leicester, UK.Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK, ceb17@le.ac.uk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30510409

Citation

George, Leena M., et al. "Sputum Moraxella Catarrhalis Strains Exhibit Diversity Within and Between COPD Subjects." International Journal of Chronic Obstructive Pulmonary Disease, vol. 13, 2018, pp. 3663-3667.
George LM, Haigh RD, Mistry V, et al. Sputum Moraxella catarrhalis strains exhibit diversity within and between COPD subjects. Int J Chron Obstruct Pulmon Dis. 2018;13:3663-3667.
George, L. M., Haigh, R. D., Mistry, V., Haldar, K., Barer, M. R., Oggioni, M. R., & Brightling, C. E. (2018). Sputum Moraxella catarrhalis strains exhibit diversity within and between COPD subjects. International Journal of Chronic Obstructive Pulmonary Disease, 13, pp. 3663-3667. doi:10.2147/COPD.S180961.
George LM, et al. Sputum Moraxella Catarrhalis Strains Exhibit Diversity Within and Between COPD Subjects. Int J Chron Obstruct Pulmon Dis. 2018;13:3663-3667. PubMed PMID: 30510409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sputum Moraxella catarrhalis strains exhibit diversity within and between COPD subjects. AU - George,Leena M, AU - Haigh,Richard D, AU - Mistry,Vijay, AU - Haldar,Koirobi, AU - Barer,Michael R, AU - Oggioni,Marco R, AU - Brightling,Christopher E, Y1 - 2018/11/08/ PY - 2018/12/5/entrez PY - 2018/12/5/pubmed PY - 2019/4/10/medline KW - COPD KW - Moraxella catarrhalis KW - exacerbation KW - strain change SP - 3663 EP - 3667 JF - International journal of chronic obstructive pulmonary disease JO - Int J Chron Obstruct Pulmon Dis VL - 13 N2 - Purpose: Moraxella catarrhalis is implicated in the pathogenesis of some COPD exacerbations. We sought to investigate whether the M. catarrhalis strain is variable between COPD subjects; that an exacerbation is associated with acquisition of a new strain and that certain strains are more commonly associated with exacerbations. Patients and methods: Sputum samples were collected at stable and exacerbation visits from COPD subjects from a single center as part of the COPDMAP consortium. Samples identified as M. catarrhalis positive by qPCR were recultured in liquid cultures grown to extract genomic DNA; underwent Illumina MiSeq and bacterial genome sequences were de novo assembled and Multi Locus Sequence Type (MLST) was determined. Results: Thirty-five samples were obtained from 18 subjects. These included 13 stable and 22 exacerbation samples. The diversity between samples was very large with 25 different M. catarrhalis MLSTs being identified out of the 35 samples of which 12 MSLTs have not been described previously. Change and persistence of M. catarrhalis strain were observed between stable visits, from stable to exacerbation and vice-a-versa, and between exacerbation visits. Conclusion: Sputum M. catarrhalis strains exhibit marked diversity within and between COPD subjects. Acquisition of a new strain is common between stable and exacerbation events such that no strain is specifically associated with an exacerbation. SN - 1178-2005 UR - https://www.unboundmedicine.com/medline/citation/30510409/Sputum_Moraxella_catarrhalis_strains_exhibit_diversity_within_and_between_COPD_subjects_ L2 - https://dx.doi.org/10.2147/COPD.S180961 DB - PRIME DP - Unbound Medicine ER -