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Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles.
Mikrochim Acta 2018; 186(1):2MA

Abstract

The sensitivity of lateral flow assays (LFA) was increased 30-fold by making use of spherical core-shell gold-silica nanoparticles (AuNP@SiO2 NPs). They can be prepared by silylation of surfactant stabilized AuNPs. The AuNP@SiO2 NPs are highly stable and can be used to label antibodies at virtually any concentration. The detection limit of an LFA for alpha-fetoprotein (AFP) can be decreased from 10 ng·mL-1 to 300 pg·mL-1 which makes it comparable to an enzyme-linked immunosorbent assay. To demonstrate the applicability to an immunoassay, a sandwich assay was developed for vanillin by covalent modification of the AuNP@SiO2 NPs with antibody. By using the method, vanillin can be detected visually in milk powder samples in concentrations as low as 100 ng·g-1. With unique optical property and great stability, this AuNP@SiO2 endows great potential in biosensing applications. Graphical abstract Controlled growth of AuNP@SiO2. The newly prepared AuNP has a negative hydration layer. This layer is further surrounded by a bilayer of CTAB through electrostatic attraction. The hydrophobic inner layer enables the access and assembling of APTES and MTTS. After the hydrolysis of siloxane, a thin layer of silica shell is formed around AuNP.

Authors+Show Affiliations

The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China.State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 South Xianlie Road, Guangzhou, 510060, China.Clinical Trials Center, Hong Kong University-ShenZhen hospital, Shenzhen, 518053, China.The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China.The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China.The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. zxkstarr@yeah.net.The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. ning.sun@connect.plolyu.hk.The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. fangjnu@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30515570

Citation

Lu, Xuewen, et al. "Improved Performance of Lateral Flow Immunoassays for Alpha-fetoprotein and Vanillin By Using Silica Shell-stabilized Gold Nanoparticles." Mikrochimica Acta, vol. 186, no. 1, 2018, p. 2.
Lu X, Mei T, Guo Q, et al. Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles. Mikrochim Acta. 2018;186(1):2.
Lu, X., Mei, T., Guo, Q., Zhou, W., Li, X., Chen, J., ... Fang, Z. (2018). Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles. Mikrochimica Acta, 186(1), p. 2. doi:10.1007/s00604-018-3107-9.
Lu X, et al. Improved Performance of Lateral Flow Immunoassays for Alpha-fetoprotein and Vanillin By Using Silica Shell-stabilized Gold Nanoparticles. Mikrochim Acta. 2018 12 4;186(1):2. PubMed PMID: 30515570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles. AU - Lu,Xuewen, AU - Mei,Ting, AU - Guo,Qi, AU - Zhou,Wenjing, AU - Li,Xiaomei, AU - Chen,Jitao, AU - Zhou,Xinke, AU - Sun,Ning, AU - Fang,Zhiyuan, Y1 - 2018/12/04/ PY - 2018/10/21/received PY - 2018/11/18/accepted PY - 2018/12/6/entrez PY - 2018/12/6/pubmed PY - 2018/12/6/medline KW - AFP KW - Core-shell gold nanoparticle KW - Lateral flow assay KW - Vanillin SP - 2 EP - 2 JF - Mikrochimica acta JO - Mikrochim Acta VL - 186 IS - 1 N2 - The sensitivity of lateral flow assays (LFA) was increased 30-fold by making use of spherical core-shell gold-silica nanoparticles (AuNP@SiO2 NPs). They can be prepared by silylation of surfactant stabilized AuNPs. The AuNP@SiO2 NPs are highly stable and can be used to label antibodies at virtually any concentration. The detection limit of an LFA for alpha-fetoprotein (AFP) can be decreased from 10 ng·mL-1 to 300 pg·mL-1 which makes it comparable to an enzyme-linked immunosorbent assay. To demonstrate the applicability to an immunoassay, a sandwich assay was developed for vanillin by covalent modification of the AuNP@SiO2 NPs with antibody. By using the method, vanillin can be detected visually in milk powder samples in concentrations as low as 100 ng·g-1. With unique optical property and great stability, this AuNP@SiO2 endows great potential in biosensing applications. Graphical abstract Controlled growth of AuNP@SiO2. The newly prepared AuNP has a negative hydration layer. This layer is further surrounded by a bilayer of CTAB through electrostatic attraction. The hydrophobic inner layer enables the access and assembling of APTES and MTTS. After the hydrolysis of siloxane, a thin layer of silica shell is formed around AuNP. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/30515570/Improved_performance_of_lateral_flow_immunoassays_for_alpha_fetoprotein_and_vanillin_by_using_silica_shell_stabilized_gold_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -