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The Effect of Mercury Chloride and Boric Acid on Rat Erythrocyte Enzymes.
Biol Trace Elem Res. 2019 Sep; 191(1):177-182.BT

Abstract

The aim of this study was to investigate the effects of mercury chloride and boric acid on rat (Wistar albino) erythrocyte: glucose 6-phosphate dehydrogenase (G6PD), 6-phosphoglucona-te dehydrogenase (6PGD), thioredoxin reductase (TrxR), glutathione reductase (GR) and glutathione S-transferase (GST) enzymes in vivo, and the rat erythrocyte G6PD enzyme in vitro. In the in vivo study, 24 male rats were divated into three different groups: control (C), mercury chloride (M), and mercury chloride + boric acid (M + BA). At the completion of this study, a significant degree of inhibition for both G6PD and GST enzyme activity was observed in the M groups when compared to the C group (p < 0.05), and no significant effect was observed in the 6PGD enzyme. However, there was significantly increased TrxR and GR enzyme activity of both the M and M + BA groups (p < 0.05). In the in vitro study, the G6PD enzyme from rat erythrocytes was purified with 2',5'-ADP Sepharose-4B affinity chromatography, and the effect of both mercury chloride and boric acid on the enzyme activity was investigated. The results showed that boric acid increased the G6PD enzyme activity while the mercury ions that inhibited the enzyme activity (IC50 values of 346 μM and Ki values of 387 μM) were noncompetitive.

Authors+Show Affiliations

Solhan Health Services Vocational School, Bingol University, 12000, Bingol, Turkey. ytemel@bingol.edu.tr.Department of Chemistry, Faculty of Arts and Sciences, Bingol University, 12000, Bingol, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30523573

Citation

Temel, Yusuf, and M Şerif Taysi. "The Effect of Mercury Chloride and Boric Acid On Rat Erythrocyte Enzymes." Biological Trace Element Research, vol. 191, no. 1, 2019, pp. 177-182.
Temel Y, Taysi MŞ. The Effect of Mercury Chloride and Boric Acid on Rat Erythrocyte Enzymes. Biol Trace Elem Res. 2019;191(1):177-182.
Temel, Y., & Taysi, M. Ş. (2019). The Effect of Mercury Chloride and Boric Acid on Rat Erythrocyte Enzymes. Biological Trace Element Research, 191(1), 177-182. https://doi.org/10.1007/s12011-018-1601-x
Temel Y, Taysi MŞ. The Effect of Mercury Chloride and Boric Acid On Rat Erythrocyte Enzymes. Biol Trace Elem Res. 2019;191(1):177-182. PubMed PMID: 30523573.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Effect of Mercury Chloride and Boric Acid on Rat Erythrocyte Enzymes. AU - Temel,Yusuf, AU - Taysi,M Şerif, Y1 - 2018/12/07/ PY - 2018/10/11/received PY - 2018/11/30/accepted PY - 2018/12/14/pubmed PY - 2019/12/25/medline PY - 2018/12/8/entrez KW - 6-phosphogluconate dehydrogenase KW - Boric acid KW - Glucose 6-phosphate dehydrogenase KW - Glutathione S-transferase KW - Glutathione reductase KW - Mercury chloride KW - Thioredoxin reductase SP - 177 EP - 182 JF - Biological trace element research JO - Biol Trace Elem Res VL - 191 IS - 1 N2 - The aim of this study was to investigate the effects of mercury chloride and boric acid on rat (Wistar albino) erythrocyte: glucose 6-phosphate dehydrogenase (G6PD), 6-phosphoglucona-te dehydrogenase (6PGD), thioredoxin reductase (TrxR), glutathione reductase (GR) and glutathione S-transferase (GST) enzymes in vivo, and the rat erythrocyte G6PD enzyme in vitro. In the in vivo study, 24 male rats were divated into three different groups: control (C), mercury chloride (M), and mercury chloride + boric acid (M + BA). At the completion of this study, a significant degree of inhibition for both G6PD and GST enzyme activity was observed in the M groups when compared to the C group (p < 0.05), and no significant effect was observed in the 6PGD enzyme. However, there was significantly increased TrxR and GR enzyme activity of both the M and M + BA groups (p < 0.05). In the in vitro study, the G6PD enzyme from rat erythrocytes was purified with 2',5'-ADP Sepharose-4B affinity chromatography, and the effect of both mercury chloride and boric acid on the enzyme activity was investigated. The results showed that boric acid increased the G6PD enzyme activity while the mercury ions that inhibited the enzyme activity (IC50 values of 346 μM and Ki values of 387 μM) were noncompetitive. SN - 1559-0720 UR - https://www.unboundmedicine.com/medline/citation/30523573/The_Effect_of_Mercury_Chloride_and_Boric_Acid_on_Rat_Erythrocyte_Enzymes_ L2 - https://dx.doi.org/10.1007/s12011-018-1601-x DB - PRIME DP - Unbound Medicine ER -
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