Tags

Type your tag names separated by a space and hit enter

Evaluation of Enzyme-Linked Immunosorbent Assay Using Recombinant 56-kDa Type-Specific Antigens Derived from Multiple Orientia tsutsugamushi Strains for Detection of Scrub Typhus Infection.
Am J Trop Med Hyg. 2019 03; 100(3):532-539.AJ

Abstract

Scrub typhus is caused by the intracellular bacterium Orientia tsutsugamushi. The 56-kDa type-specific antigen (TSA) displays a significant antigenic variation across different O. tsutsugamushi strains. To minimize the influence of the antigenic diversity of TSA on assay sensitivity, we developed a mixed-TSA enzyme-linked immunosorbent assay (mixed-TSA ELISA) using a mixture of recombinant TSAs of prototype (Karp, Gilliam, and Kato) and local (TW-1, TW-10, TW-19, and TW-22) O. tsutsugamushi strains as antigens to detect immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against O. tsutsugamushi. These four local strains covered a major part of the total genetic diversity of TSA gene of O. tsutsugamushi in Taiwan. A total of 109 acute-phase serum samples from O. tsutsugamushi polymerase chain reaction-positive, scrub typhus patients, and 82 negative control serum samples from non-scrub typhus cases were used for evaluation of the recombinant TSA-based ELISA. We compared the performance of the mixed-TSA ELISA with immunofluorescence assay (IFA), which is considered the gold standard method for the serological diagnosis of scrub typhus. The results indicated that the sensitivity of IgM mixed-TSA ELISA (80.7%) was significantly higher than that of IgM IFA (68.8%). We demonstrated that the mixed-TSA ELISA had a high sensitivity and specificity and can be used for screening of scrub typhus patient in the early phase of the disease.

Authors+Show Affiliations

Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taipei, Republic of China.Department of Public Health and Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei, Republic of China.Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taipei, Republic of China.Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taipei, Republic of China.Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taipei, Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30526730

Citation

Yang, Su-Lin, et al. "Evaluation of Enzyme-Linked Immunosorbent Assay Using Recombinant 56-kDa Type-Specific Antigens Derived From Multiple Orientia Tsutsugamushi Strains for Detection of Scrub Typhus Infection." The American Journal of Tropical Medicine and Hygiene, vol. 100, no. 3, 2019, pp. 532-539.
Yang SL, Tsai KH, Chen HF, et al. Evaluation of Enzyme-Linked Immunosorbent Assay Using Recombinant 56-kDa Type-Specific Antigens Derived from Multiple Orientia tsutsugamushi Strains for Detection of Scrub Typhus Infection. Am J Trop Med Hyg. 2019;100(3):532-539.
Yang, S. L., Tsai, K. H., Chen, H. F., Luo, J. Y., & Shu, P. Y. (2019). Evaluation of Enzyme-Linked Immunosorbent Assay Using Recombinant 56-kDa Type-Specific Antigens Derived from Multiple Orientia tsutsugamushi Strains for Detection of Scrub Typhus Infection. The American Journal of Tropical Medicine and Hygiene, 100(3), 532-539. https://doi.org/10.4269/ajtmh.18-0391
Yang SL, et al. Evaluation of Enzyme-Linked Immunosorbent Assay Using Recombinant 56-kDa Type-Specific Antigens Derived From Multiple Orientia Tsutsugamushi Strains for Detection of Scrub Typhus Infection. Am J Trop Med Hyg. 2019;100(3):532-539. PubMed PMID: 30526730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Enzyme-Linked Immunosorbent Assay Using Recombinant 56-kDa Type-Specific Antigens Derived from Multiple Orientia tsutsugamushi Strains for Detection of Scrub Typhus Infection. AU - Yang,Su-Lin, AU - Tsai,Kun-Hsien, AU - Chen,Hsiang-Fei, AU - Luo,Jun-Yu, AU - Shu,Pei-Yun, PY - 2018/12/12/pubmed PY - 2020/1/7/medline PY - 2018/12/12/entrez SP - 532 EP - 539 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 100 IS - 3 N2 - Scrub typhus is caused by the intracellular bacterium Orientia tsutsugamushi. The 56-kDa type-specific antigen (TSA) displays a significant antigenic variation across different O. tsutsugamushi strains. To minimize the influence of the antigenic diversity of TSA on assay sensitivity, we developed a mixed-TSA enzyme-linked immunosorbent assay (mixed-TSA ELISA) using a mixture of recombinant TSAs of prototype (Karp, Gilliam, and Kato) and local (TW-1, TW-10, TW-19, and TW-22) O. tsutsugamushi strains as antigens to detect immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against O. tsutsugamushi. These four local strains covered a major part of the total genetic diversity of TSA gene of O. tsutsugamushi in Taiwan. A total of 109 acute-phase serum samples from O. tsutsugamushi polymerase chain reaction-positive, scrub typhus patients, and 82 negative control serum samples from non-scrub typhus cases were used for evaluation of the recombinant TSA-based ELISA. We compared the performance of the mixed-TSA ELISA with immunofluorescence assay (IFA), which is considered the gold standard method for the serological diagnosis of scrub typhus. The results indicated that the sensitivity of IgM mixed-TSA ELISA (80.7%) was significantly higher than that of IgM IFA (68.8%). We demonstrated that the mixed-TSA ELISA had a high sensitivity and specificity and can be used for screening of scrub typhus patient in the early phase of the disease. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/30526730/Evaluation_of_Enzyme_Linked_Immunosorbent_Assay_Using_Recombinant_56_kDa_Type_Specific_Antigens_Derived_from_Multiple_Orientia_tsutsugamushi_Strains_for_Detection_of_Scrub_Typhus_Infection_ DB - PRIME DP - Unbound Medicine ER -