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LncRNA uc003fir promotes CCL5 expression and negatively affects proliferation and migration of trophoblast cells in preeclampsia.
Pregnancy Hypertens 2018; 14:90-96PH

Abstract

OBJECTIVE

To evaluate the role of lncRNA uc003fir in the progression and development of preeclampsia (PE).

DESIGN

Paired test using placental tissue collected from PE women and normally pregnant women.

SETTING

First Hospital of Soochow University, Suzhou, China.

POPULATION

38 women with PE versus 42 normally pregnant women matched maternal and gestational age with the same-size ratio.

METHODS

Placental samples were analyzed using a human lncRNA microarray, and then the lncRNA uc003fir was validated by q-PCR. MTT, cell scratch, and transwell invasion assays were performed regarding biological functions.

MAIN OUTCOME MEASURES

LncRNA uc003fir promoted CCL5 expression and negatively affected the proliferation and migration of HTR-8/SVneo cells.

RESULTS

LncRNA uc003fir was significantly over-expressed in PE compared to the control. The chemokine CCL5 was directly regulated by lncRNA uc003fir through transfection of over-expression plasmid of lncRNA uc003fir in HTR-8/SVneo cells, accompanied by up-regulation of lncRNA uc003fir in preeclamptic placental vessels and vessel-free tissue. In detecting the biological function of lncRNA uc003fir on trophoblast cells, over-expression of lncRNA uc003fir increased proliferation, migration, and invasion of HTR-8/SVneo cells, whereas the knockdown inhibited.

CONCLUSION

LncRNA uc003fir was dysregulated in placental tissue in PE. LncRNA uc003fir regulated CCL5 directly, which may mediate the recruitment of pro-inflammatory cytokines released from monocytes and other leukocytes. Additionally, lncRNA uc003fir may interact with microRNAs to affect the invasion of trophoblast cells into myometrium, indicating that lncRNA uc003fir could be considered as a target in PE.

Authors+Show Affiliations

Institute for Fetology, First Hospital of Soochow University, Suzhou, China.Department of Emergency Surgery, First Hospital of Soochow University, Suzhou, China.Department of Obstetrics and Gynecology, First Hospital of Soochow University, Suzhou, China.Institute for Fetology, First Hospital of Soochow University, Suzhou, China.Institute for Fetology, First Hospital of Soochow University, Suzhou, China.Department of Obstetrics and Gynecology, Municipal Hospital, Suzhou, China. Electronic address: jannetao@163.com.Institute for Fetology, First Hospital of Soochow University, Suzhou, China; Centre for Prenatal Biology, Loma Linda University, CA, USA. Electronic address: xuzhice@suda.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30527126

Citation

Pengjie, Zhang, et al. "LncRNA Uc003fir Promotes CCL5 Expression and Negatively Affects Proliferation and Migration of Trophoblast Cells in Preeclampsia." Pregnancy Hypertension, vol. 14, 2018, pp. 90-96.
Pengjie Z, Xionghui C, Yueming Z, et al. LncRNA uc003fir promotes CCL5 expression and negatively affects proliferation and migration of trophoblast cells in preeclampsia. Pregnancy Hypertens. 2018;14:90-96.
Pengjie, Z., Xionghui, C., Yueming, Z., Ting, X., Na, L., Jianying, T., & Zhice, X. (2018). LncRNA uc003fir promotes CCL5 expression and negatively affects proliferation and migration of trophoblast cells in preeclampsia. Pregnancy Hypertension, 14, pp. 90-96. doi:10.1016/j.preghy.2018.08.449.
Pengjie Z, et al. LncRNA Uc003fir Promotes CCL5 Expression and Negatively Affects Proliferation and Migration of Trophoblast Cells in Preeclampsia. Pregnancy Hypertens. 2018;14:90-96. PubMed PMID: 30527126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LncRNA uc003fir promotes CCL5 expression and negatively affects proliferation and migration of trophoblast cells in preeclampsia. AU - Pengjie,Zhang, AU - Xionghui,Chen, AU - Yueming,Zhang, AU - Ting,Xu, AU - Na,Li, AU - Jianying,Tao, AU - Zhice,Xu, Y1 - 2018/08/17/ PY - 2018/04/04/received PY - 2018/07/29/revised PY - 2018/08/15/accepted PY - 2018/12/12/entrez PY - 2018/12/12/pubmed PY - 2019/1/10/medline KW - CCL5 KW - HTR-8/SVneo cells KW - Preeclampsia KW - lncRNA uc003fir SP - 90 EP - 96 JF - Pregnancy hypertension JO - Pregnancy Hypertens VL - 14 N2 - OBJECTIVE: To evaluate the role of lncRNA uc003fir in the progression and development of preeclampsia (PE). DESIGN: Paired test using placental tissue collected from PE women and normally pregnant women. SETTING: First Hospital of Soochow University, Suzhou, China. POPULATION: 38 women with PE versus 42 normally pregnant women matched maternal and gestational age with the same-size ratio. METHODS: Placental samples were analyzed using a human lncRNA microarray, and then the lncRNA uc003fir was validated by q-PCR. MTT, cell scratch, and transwell invasion assays were performed regarding biological functions. MAIN OUTCOME MEASURES: LncRNA uc003fir promoted CCL5 expression and negatively affected the proliferation and migration of HTR-8/SVneo cells. RESULTS: LncRNA uc003fir was significantly over-expressed in PE compared to the control. The chemokine CCL5 was directly regulated by lncRNA uc003fir through transfection of over-expression plasmid of lncRNA uc003fir in HTR-8/SVneo cells, accompanied by up-regulation of lncRNA uc003fir in preeclamptic placental vessels and vessel-free tissue. In detecting the biological function of lncRNA uc003fir on trophoblast cells, over-expression of lncRNA uc003fir increased proliferation, migration, and invasion of HTR-8/SVneo cells, whereas the knockdown inhibited. CONCLUSION: LncRNA uc003fir was dysregulated in placental tissue in PE. LncRNA uc003fir regulated CCL5 directly, which may mediate the recruitment of pro-inflammatory cytokines released from monocytes and other leukocytes. Additionally, lncRNA uc003fir may interact with microRNAs to affect the invasion of trophoblast cells into myometrium, indicating that lncRNA uc003fir could be considered as a target in PE. SN - 2210-7797 UR - https://www.unboundmedicine.com/medline/citation/30527126/LncRNA_uc003fir_promotes_CCL5_expression_and_negatively_affects_proliferation_and_migration_of_trophoblast_cells_in_preeclampsia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2210-7789(18)30097-7 DB - PRIME DP - Unbound Medicine ER -